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2.4.1.65: 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase

This is an abbreviated version!
For detailed information about 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.65

Reaction

GDP-beta-L-fucose
+
beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R
=
GDP
 +
beta-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->4)]-N-acetyl-beta-D-glucosaminyl-R

Synonyms

(Lea)-dependent alpha-3/4-fucosyltransferase, alpha 1,3-fucosyltransferase VII, alpha 1,3/4 fucosyltransferase, alpha(1,3) fucosyltransferase, alpha(1,3/1,4) fucosyltransferase III, alpha(1,3/4) fucosyltransferase, alpha(1,3/4)-FUT, alpha-(1,3/1,4)-fucosyltransferase, alpha-(1,3/1,4)-FucT, alpha-(1-3/4)-fucosyltransferase, alpha-(1->4)-L-fucosyltransferase, alpha-1,3 fucosyltransferase, alpha-1,3-fucosyltransferase, alpha-1,3/4-fucosyltransferase, alpha-1,3fucosyltransferase, alpha-1,4 fucosyltransferase, alpha-3-FUT, alpha-3/4 fucosyltransferase, alpha-3/4 fucosyltransferase III, alpha-3/4-FUT, alpha-4-fucosyltransferase, alpha-4-L-fucosyltransferase, alpha1,3 fucosyltransferase, alpha1,3 fucosyltransferase VII, alpha1,3-fucosyltransferase, alpha1,3-fucosyltransferase IX, alpha1,3-fucosyltransferase VI, alpha1,3/1,4FucT III, alpha1,3/4-fucosyltransferase, alpha1,3/4-fucosyltransferase VI, alpha1,3/4-fucosyltransferases-V and -VI, alpha1,3/4-FUT, alpha1,3FucT IV, alpha1,3FucT IX, alpha1,3FucT V, alpha1,3FucT VI, alpha1,3FucT VII, alpha3/4 fucosyltransferase 3, alpha3/4 fucosyltransferases, alpha3/4FucT, alpha4-fucosyltransferase, alpha4-FucT, alphaFucT, beta-acetylglucosaminylsaccharide fucosyltransferase, blood group Lewis alpha-4-fucosyltransferase, blood-group substance Lea-dependent fucosyltransferase, FT-IV, FT-VII, FT3, FT6, FT7, Fuc-T, Fuc-TIII, fucosyltransferase, fucosyltransferase 3, fucosyltransferase 5, fucosyltransferase 6, fucosyltransferase 7, fucosyltransferase IV, fucosyltransferase V, fucosyltransferase VI, fucosyltransferase VII, fucosyltransferase, guanosine diphosphofucose-beta-acetylglucosaminylsaccharide 4-alpha-L-, fucosyltransferase, guanosine diphosphofucose-glycoprotein 4-alpha-, FucT, FucT III, FucT IV, FucT IX, FucT V, FucT VI, FucT VII, FucT-II, FucT-III, FucT-V, FucT-VI, FucT-VII, FucTIII, FUT III, FUT10, FUT11, Fut13, FUT3, FUT4, FUT5, FUT6, FUT7, Fut9, GDP-Fuc: beta-D-Galp-(1-3/4)-beta-D-GlcpNAc-(1-4'/3')-alpha-fucosyltransferase, guanosine diphosphofucose-glycoprotein 4-alpha-L-fucosyltransferase, Lewis alpha-3-fucosyltransferase, Lewis alpha-3/4-fucosyltransferase, Lewis alpha1-3/4 fucosyltransferase, Lewis blood group alpha1-3/4 fucosyltransferase, Lewis(Le) blood group gene-dependent alpha-3/4-L-fucosyltransferase, SFT3, sFUT5, sperm fucosyltransferase-5, UA948FucT

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.65 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase

Engineering

Engineering on EC 2.4.1.65 - 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D112E
-
muation decreases activity of the enzyme and does not interfere with H-type 1/H-type 2 acceptors
D125E
-
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity
D336A
-
fucosyltransferase III mutant enzyme shows reduced activity with a variety of acceptors, 40fold reduction in activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc. 4fold reduction affinity for GDP-fucose. The single amino acid site Asp336 of FucT III and Ala349 of FucT V constitutes the only difference in the sequence of FucT III and V over the final 210 COOH-terminal amino acid residues, impacts the acceptor substrate profiles of FucT III and FuvT V
E111D
-
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity
I126V
-
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity
K73T
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
K73T/E111D
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
K73T/I75V/T87A
-
insertion mutagenesis of FucT-VI, the mutant shows altered substrate specificity compared to the wild-type enzyme
K73T/R110W
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
K73T/R110W/E111D/V112I
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
K97S
-
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity
R110H
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity
R110K
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity
R110N
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity
R110Q
R110W
R110W/E111D/V112I
-
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme
R96S
-
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity
S110R
-
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity
S111K
-
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity
T87K
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
T87K/D125E
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
T87K/V89I/A101T
-
insertion mutagenesis of FucT-V, the mutant shows altered substrate specificity compared to the wild-type enzyme
T87K/W124R
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
T87K/W124R/D125E/I126V
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
V112I
-
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity
W111A
alpha-1,4-activity is decreased to 10-20% of the wild type activity
W111F
alpha-1,4-activity is decreased to 42% of the wild type activity
W111R
-
the mutation changes the specificity for fucose transfer from H-type 1 to H-type 2 acceptors
W111Y
alpha-1,4-activity is decreased to 58% of the wild type activity
W11R/D112E
-
the mutation changes the specificity for fucose transfer from H-type 1 to H-type 2 acceptors. Increased type 2 activity compared to mutant W111R
W124A
alpha-1,4-activity is decreased to 20% of the wild type activity
W124F
alpha-1,4-activity is decreased to 17% of the wild type activity
W124R
W124R/D125E/I126V
-
site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme
W124V
undetectable alpha-1,4-activity
W124Y
alpha-1,4-activity is decreased to 52% of the wild type activity
FT3dc
-
mutant where the cytoplasmic domain (Asp-2 to Trp-13) is deleted
additional information