the enzyme is a candidate for the earliest marker of megakaryocyte-commitment in cultured hematopoietic stem cells. Such a marker should allow an earlier detection and earlier transplantation of patients own, ex vivo expanded, megakaryocyte progenitors
the enzymatic product of core alpha1,6-fucosyltransferase plays a major role in a plethora of pathological conditions, e.g. in prognosis of hepatocellular carcinoma and in colon cancer
mutants of the general acid/base residue E274, including E274A, E274S, and E274G, act as efficient glycoligases able to fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins by using alpha-fucosyl fluoride as a simple donor substrate. The alpha-1,6-fucosidase mutants can introduce an alpha1,6-fucose moiety specifically at the Asn-linked GlcNAc moiety to GlcNAc-peptide, and also to high-mannose and complex type N-glycans in the context of N-glycopeptides, N-glycoproteins, and intact antibodies
mutants of the general acid/base residue E274, including E274A, E274S, and E274G, act as efficient glycoligases able to fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins by using alpha-fucosyl fluoride as a simple donor substrate. The alpha-1,6-fucosidase mutants can introduce an alpha1,6-fucose moiety specifically at the Asn-linked GlcNAc moiety to GlcNAc-peptide, and also to high-mannose and complex type N-glycans in the context of N-glycopeptides, N-glycoproteins, and intact antibodies