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2.4.1.80: ceramide glucosyltransferase

This is an abbreviated version!
For detailed information about ceramide glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.80

Reaction

UDP-alpha-D-glucose
+
an N-acylsphingosine
=
UDP
 +
a beta-D-glucosyl-N-acylsphingosine

Synonyms

ceramide glucosyltransferase, ceramide glycosyltransferase, ceramide:UDP-glucose glucosyltransferase, ceramide:UDPGlc glucosyltransferase, cerebroside synthase, CGT, cgt-1, cgt-2, cgt-3, GCS, GCS1, GlcCer synthase, GlcCerS, GlcT, GlcT-1, glucosylceramide synthase, glucosyltransferase, uridine diphosphoglucose-ceramide, GluT, UDP glucose-ceramide glucosyltransferase, UDP-Glc:ceramide glucosyltransferase, UDP-glucoase:ceramide glucosyltransferase, UDP-glucose ceramide glucosyltransferase, UDP-glucose ceramide glycosyltransferase, UDP-glucose: ceramide glucosyltransferase, UDP-glucose:ceramide glucosyltransferase, Ugcg, uridine diphosphate (UDP)-glucose:N-acylsphingosine D-glucosyltransferase, uridine diphosphoglucose-ceramide glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.80 ceramide glucosyltransferase

Cloned

Cloned on EC 2.4.1.80 - ceramide glucosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and transient expression in GM-95 cell line, mutant cell line containing no enzyme activity, derived from melanoma mutant cell line B16, DNA sequence determination and analysis
cloning from CHO cell line K1, overexpression in GM3-mutant CHO cell line Z65, DNA and amino acid sequence determination
-
cloning from rat brain genetic library and expression in Escherichia coli BL21(DE3)
cloning of wild-type and construction of knock-out mutant, functional expression in Saccharomyces cerevisiae strain 334, expression of wild-type leads to accumulation of beta-D-glucopyranosyl ceramides, absent in wild-type Saccharomyces cerevisiae, knock-out mutation is not lethal
-
expressed as recombinant protein
-
expressed in different drug-resistant human cancer cell lines
-
expressed in GM95 cells deficient in glycosphingolipids
-
expressed in PC12 cells
-
expression in an enzyme-deficient strain of Pichia pastoris
-
expression in MCF-7 cell
expression in Saccharomyces cerevisiae
Q58FH5
expression of enzyme gene in antisense orientation, two-hybrid system: coexpression with the reticulon RTN family, interaction especially with RTN-1C
-
expression of wild-type and mutants in Escherichia coli BL21(DE3)
functional expression in Escherichia coli BL21(DE3)
functional expression in membranes of a Pichia pastoris enzyme-deficient double-null mutant strain, also lacking sterol glucosyltransferase activity
-
functional expression in Saccharomyces cerevisiae strain 334
-
functional expression of 1 isozyme in Saccharomyces cerevisiae strain 334
-
gene GlcT-1, DNA sequence determination and analysis, functional expression in enzyme-deficient GM-95 cells, expression as V5/His-tagged protein in GM95 cells
-
gene GluT-1 cloned from a human melanoma genetic library, transient overexpression in HL-60 and HL-60/ADR cells, induced resistance to DOX in HL-60 cells
-
overexpression in cancer cell lines selected for resistance to natural product chemotherapy, i.e. cell lines KB-3-1, KB-V.01, KB-V.1, KB-V1, KB-A.05, MCF-7, and MCF-7-AdrR, cell lines show different recombinant enzyme activity, which is higherin cells resistant to natural product anticancer agents, e.g. vinblastine and adriamycin, overview
-