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2.4.1.9: inulosucrase

This is an abbreviated version!
For detailed information about inulosucrase, go to the full flat file.

Word Map on EC 2.4.1.9

Reaction

sucrose
+
[(beta-D-Fruf-(2->1))n]-alpha-D-Glup
=
alpha-D-glucose
 +
[(beta-D-Fruf-(2->1))n+1]-alpha-D-Glup

Synonyms

Ffase, fructansucrase, fructosyltransferase, fructosyltransferase, sucrose 1-, FTase, FTF, GH68 fructansucrase, HugO, INU, InuJ, IS, ISase, IslA, More, sucrose 1-fructosyltransferase, sucrose: 2,1-beta-D-fructan 1-beta-D-fructosyltransferase, sucrose:2,1-beta-D-fructan1-beta-D-fructosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.9 inulosucrase

Cloned

Cloned on EC 2.4.1.9 - inulosucrase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Bacillus subtilis
expressed in Escherichia coli
expressed in Escherichia coli and Bacillus subtilis
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli NiCo21(DE3) cells
-
expression in Escherichia coli
expression of His-tagged wild-type and mutant enzymes in Escherichia coli TOP10 cells
expression of Myc/His-tagged enzyme in Escherichia coli
expression of Myc/His-tagged wild-type and mutant enzymes in Escherichia coli
expression of the full-length enzyme and two C-terminally truncated enzyme forms in Escherichia coli. Natural chimeric enzyme with three domains: the first and third with high identity with alternansucrase, the second domain, which is the catalytic one has similarity with levansucrase and inulosucrase
-
expression of the full-length protein and a truncated protein with a 100 amino acid truncation at the C-terminus from amino acid 699 onwards, with a C-terminal His-tag, InuDELTA699His
-
gene inuGB, recombinant expression of His6-tagged truncated enzyme mutant lacking the cell-anchoring motif in Escherichia coli strain DH10B
recombinant expression of C-terminally His-tagged fragment of inulosucrase comprising residues 145-708
-
recombinant expression of His-tagged full-length wild-type enzyme and truncated mutant enzyme in Escherichia coli strain BL21
-
recombinant overexpression of the enzyme lacking the cell-anchoring motif in Escherichia coli
-
the N- and C-terminally truncated enzyme is expressed in Saccharomyces cerevisiae strain 2805gs
the truncated mutant IslA4 is expressed in Escherichia coli BL21(DE3) cells
-