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2.4.1.9: inulosucrase

This is an abbreviated version!
For detailed information about inulosucrase, go to the full flat file.

Word Map on EC 2.4.1.9

Reaction

sucrose
+
[(beta-D-Fruf-(2->1))n]-alpha-D-Glup
=
alpha-D-glucose
 +
[(beta-D-Fruf-(2->1))n+1]-alpha-D-Glup

Synonyms

Ffase, fructansucrase, fructosyltransferase, fructosyltransferase, sucrose 1-, FTase, FTF, GH68 fructansucrase, HugO, INU, InuJ, IS, ISase, IslA, More, sucrose 1-fructosyltransferase, sucrose: 2,1-beta-D-fructan 1-beta-D-fructosyltransferase, sucrose:2,1-beta-D-fructan1-beta-D-fructosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.9 inulosucrase

Specific Activity

Specific Activity on EC 2.4.1.9 - inulosucrase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00032
-
purified recombinant mutant D272N, inulin synthesizing activity
0.00066
-
purified recombinant mutant D424N, inulin synthesizing activity
0.0028
-
purified recombinant mutant E523Q, inulin synthesizing activity
0.3
below, purified recombinant mutant D520A, 50°C, without Ca2+
1
purified recombinant mutant D520N, 50°C, 1 mM Ca2+
10.5
purified recombinant truncated His-tagged enzyme, pH 5.2, 55°C
178
hydrolytic activity, 37°C, pH 7.0
180
purified recombinant wild-type enzyme, 50°C, 1 mM Ca2+
187
transglycosylation activity, 37°C, pH 7.0
27
hydrolytic activity, 55°C, pH 7.0
3.6
purified recombinant mutant D520A, 50°C, 1 mM Ca2+
365
total activity, 37°C, pH 7.0
459
-
pH 5.5, 55°C
47
-
purified recombinant wild-type enzyme, inulin synthesizing activity
698
transglycosylation activity, 55°C, pH 7.0
719
total activity, 55°C, pH 7.0