2.4.1.B46: 4-O-(alpha-L-rhodosaminyl)aklavinone L-2-deoxyfucosyltransferase

This is an abbreviated version!
For detailed information about 4-O-(alpha-L-rhodosaminyl)aklavinone L-2-deoxyfucosyltransferase, go to the full flat file.

Reaction

Synonyms

AknK

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.B46 4-O-(alpha-L-rhodosaminyl)aklavinone L-2-deoxyfucosyltransferase

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Results	in table
1	AA Sequence
1	Application
1	Cloned(Commentary)
6	kcat/KM [mM/s]
6	KM Value [mM]
1	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
2	Organism
2	pH Optimum
1	Purification (Commentary)
1	Reaction
2	Reference
8	Substrates and Products (Substrate)
1	Subunits
1	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]

Substrates Products

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Substrates Products on EC 2.4.1.B46 - 4-O-(alpha-L-rhodosaminyl)aklavinone L-2-deoxyfucosyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

dTDP-2-deoxy-alpha-L-fucose + 4-O-[(4-O-2-deoxy-alpha-L-fucosyl)-alpha-L-rhodosaminyl]aklavinone

dTDP + 4-O-[[4-O-2-deoxy-alpha-L-fucosyl-(4-O-alpha-L-2-deoxyfucosyl)]alpha-L-rhodosaminyl]aklavinone

Streptomyces galilaeus

Q9L555

AknK also catalyzes a tandem addition of a second L-2-deoxyfucosyl moiety, albeit with reduced activity, to the natural disaccharide chain to produce L-deoxyfucosyl-L-deoxyfucosyl-L-rhodosaminyl aklavinone, a variant of the natural aclacinomycin A. The second transfer reaction is 3 orders of magnitude slower than the first sugar transfer reaction catalyzed by AknK

724267

-

-

?

dTDP-2-deoxy-alpha-L-fucose + 4-O-[(4-O-2-deoxy-alpha-L-fucosyl)-alpha-L-rhodosaminyl]aklavinone

dTDP + 4-O-[[4-O-alpha-L-2-deoxyfucosyl-(4-O-2-deoxy-alpha-L-fucosyl)]alpha-L-rhodosaminyl]aklavinone

Streptomyces galilaeus

-

-

725187

-

-

?

dTDP-2-deoxy-alpha-L-fucose + idarubicin

dTDP + 4'-O-(2-deoxy-alpha-L-fucosyl)idarubicin

Streptomyces galilaeus

Q9L555

-

724267

-

-

?

dTDP-2-deoxy-beta-L-fucose + 4-O-(alpha-L-rhodosaminyl)aklavinone

dTDP + 4-O-[(4-O-2-deoxy-alpha-L-fucosyl)-alpha-L-rhodosaminyl]aklavinone

2 entries

dTDP-L-daunosamine + 4-O-(alpha-L-rhodosaminyl)aklavinone

dTDP + 4-O-[(4-O-alpha-L-daunosaminyl)-alpha-L-rhodosaminyl]aklavinone

Streptomyces galilaeus

Q9L555

the enzyme accepts L-daunosamine as an alternative sugar substrate, albeit with a 270fold decrease in catalytic efficiency (kcat/Km)

724267

-

-

?

additional information

?

-

2 entries

dTDP-2-deoxy-beta-L-fucose + 4-O-(alpha-L-rhodosaminyl)aklavinone

dTDP + 4-O-[(4-O-2-deoxy-alpha-L-fucosyl)-alpha-L-rhodosaminyl]aklavinone

Streptomyces galilaeus

-

-

725187

-

-

?

dTDP-2-deoxy-beta-L-fucose + 4-O-(alpha-L-rhodosaminyl)aklavinone

dTDP + 4-O-[(4-O-2-deoxy-alpha-L-fucosyl)-alpha-L-rhodosaminyl]aklavinone

Streptomyces galilaeus

Q9L555

-

724267

-

-

?

additional information

?

-

Streptomyces galilaeus

Q9L555

AknK also accepts an alternate dTDP-L-sugar, dTDP-L-daunosamine, and other monoglycosylated anthracyclines, including daunomycin, adriamycin, and idarubicin, to build alternate disaccharides on variant anthracycline backbones

724267

-

-

?

additional information

?

-

Streptomyces galilaeus

-

the enzyme does not transfer TDP-L-rhodosamine

725187

-

-

?