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2.4.2.1: purine-nucleoside phosphorylase

This is an abbreviated version!
For detailed information about purine-nucleoside phosphorylase, go to the full flat file.

Word Map on EC 2.4.2.1

Reaction

purine 2'-deoxyribonucleoside
+
phosphate
=
purine
 +
2-deoxy-alpha-D-ribose 1-phosphate

Synonyms

5'-deoxy-5'-methylthioadenosine phosphorylase II, 5'-methythioadenosine phosphorylase, adenine nucleoside phosphorylase, adenosine phosphorylase, AgPNP, AmPNP, ANP, ApMTAP, apPNP, bovPNP, bPNO, CKP1, cytokinin riboside phosphorylase, DeoD, deoD_1, DgPNP, EC592_06680, EcPNP, flB, GsPNPI, GsPNPII, GtPNP, hPNP, HpPNP, HsPNO, HsPNP, Ino-Guo phosphorylase, inosine phosphorylase, inosine-guanosine phosphorylase, KlacPNP, KLLA0_C16621g, MTAPII, MtbPNP, nucleotide phosphatase (2.4.2.1), PfPNP, phosphorylase, purine nucleoside, PiPNP, PNP, PNP 1, PNP 2, PNP I, PNP II, PNP-II, PNP-Zg, PNPase, pNPI, PNPII, Pu-NPase, punA, PUNP, PUNPI, PUNPII, purine deoxynucleoside phosphorylase, purine deoxyribonucleoside phosphorylase, purine nucleoside orthophosphate ribosyl transferase, purine nucleoside phosphorylase, purine nucleoside phosphorylase 1, purine nucleoside phosphorylase 2, purine nucleoside phosphorylase DeoD, purine nucleoside phosphorylase DeoD-type, purine nucleoside phosphorylase II, purine ribonucleoside phosphorylase, purine-nucleoside phosphorylases II, SalT, SSO2343, TgPNP, TTC0194, TTC1070, TtPNPI, TtPNPII, TvPNP, xanthosine phosphorylase, XAP, xapA, ylmD

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.1 purine-nucleoside phosphorylase

Purification

Purification on EC 2.4.2.1 - purine-nucleoside phosphorylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
chromatofocussing column chromatography and gel filtration
-
His-Talon column chromatography
-
Ni-NTA affinity column chromatography, and gel filtration
Ni-NTA column chromatography
partial
Q-Sepharose column chromatography and Sephacryl S-200 gel filtration
recombinant
recombinant enzyme
recombinant enzyme by anion exchange chromatography followed by affinity chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3)/pERPUPHOI cell extract supernatant by anion exchange chromatography, ultrafiltration, and gel filtration
recombinant enzyme PNP-Zg from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by formycin affinity chromatography (elution with inosine), dialysis, and gel filtration, to homogeneity
recombinant N- and C-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, dialysis, ultrafiltration, and gel filtration
recombinant protein
Halalkalibacterium halodurans
Toyopearl Phenyl column chromatography, Toyopearl SuperQ column chromatography, and Superdex 200 gel filtration
-
two purine nucleoside phosphorylases: 1. inosine-guanosine phosphorylase, 2. adenosine-specific phosphorylase
-
wild-type and recombinant mutant enzymes
-