2.4.2.1: purine-nucleoside phosphorylase
This is an abbreviated version!
For detailed information about purine-nucleoside phosphorylase, go to the full flat file.
Word Map on EC 2.4.2.1
-
2.4.2.1
-
p-nitrophenol
-
deaminase
-
polynucleotide
-
lymphocyte
-
hypoxanthine
-
phosphorolysis
-
rnase
-
salvage
-
guanine
-
paraneoplastic
-
t-cells
-
pemphigus
-
immunodeficiency
-
pincer
-
deoxyguanosine
-
exoribonuclease
-
phosphoribosyltransferase
-
polyneuropathy
-
5'-nucleotidase
-
practitioner
-
dgtp
-
nurse
-
degradosome
-
hypoxanthine-guanine
-
parathion
-
blister
-
bullous
-
mucocutaneous
-
ribonucleosides
-
desmogleins
-
deoxynucleosides
-
deoxycytidine
-
hgprt
-
phrenic
-
castleman
-
deoxyadenosine
-
2'-deoxyguanosine
-
metal-ligand
-
obliterans
-
pneumoperitoneum
-
desmoplakins
-
pemphigoid
-
square-planar
-
photocatalysts
-
lesch-nyhan
-
iridium
-
photocatalytic
-
acantholysis
-
medicine
-
exonucleolytic
-
p-aminophenol
-
drug development
-
analysis
-
pharmacology
-
synthesis
- 2.4.2.1
- p-nitrophenol
- deaminase
- polynucleotide
- lymphocyte
- hypoxanthine
-
phosphorolysis
- rnase
-
salvage
- guanine
-
paraneoplastic
- t-cells
- pemphigus
- immunodeficiency
-
pincer
- deoxyguanosine
- exoribonuclease
- phosphoribosyltransferase
- polyneuropathy
- 5'-nucleotidase
-
practitioner
- dgtp
- nurse
-
degradosome
-
hypoxanthine-guanine
- parathion
- blister
-
bullous
-
mucocutaneous
- ribonucleosides
-
desmogleins
- deoxynucleosides
- deoxycytidine
- hgprt
-
phrenic
- castleman
- deoxyadenosine
- 2'-deoxyguanosine
-
metal-ligand
- obliterans
- pneumoperitoneum
- desmoplakins
- pemphigoid
-
square-planar
-
photocatalysts
- lesch-nyhan
-
iridium
-
photocatalytic
- acantholysis
- medicine
-
exonucleolytic
- p-aminophenol
- drug development
- analysis
- pharmacology
- synthesis
Reaction
Synonyms
5'-deoxy-5'-methylthioadenosine phosphorylase II, 5'-methythioadenosine phosphorylase, adenine nucleoside phosphorylase, adenosine phosphorylase, AgPNP, AmPNP, ANP, ApMTAP, apPNP, bovPNP, bPNO, CKP1, cytokinin riboside phosphorylase, DeoD, deoD_1, DgPNP, EC592_06680, EcPNP, flB, GsPNPI, GsPNPII, GtPNP, hPNP, HpPNP, HsPNO, HsPNP, Ino-Guo phosphorylase, inosine phosphorylase, inosine-guanosine phosphorylase, KlacPNP, KLLA0_C16621g, MTAPII, MtbPNP, nucleotide phosphatase (2.4.2.1), PfPNP, phosphorylase, purine nucleoside, PiPNP, PNP, PNP 1, PNP 2, PNP I, PNP II, PNP-II, PNP-Zg, PNPase, pNPI, PNPII, Pu-NPase, punA, PUNP, PUNPI, PUNPII, purine deoxynucleoside phosphorylase, purine deoxyribonucleoside phosphorylase, purine nucleoside orthophosphate ribosyl transferase, purine nucleoside phosphorylase, purine nucleoside phosphorylase 1, purine nucleoside phosphorylase 2, purine nucleoside phosphorylase DeoD, purine nucleoside phosphorylase DeoD-type, purine nucleoside phosphorylase II, purine ribonucleoside phosphorylase, purine-nucleoside phosphorylases II, SalT, SSO2343, TgPNP, TTC0194, TTC1070, TtPNPI, TtPNPII, TvPNP, xanthosine phosphorylase, XAP, xapA, ylmD
ECTree
2.4.2.1 purine-nucleoside phosphorylaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.4.2.1 - purine-nucleoside phosphorylase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,6-dihydropurine riboside + phosphate
alpha-D-ribose 1-phosphate + 1,6-dihydropurine
-
-
-
-
?
1,N2-ethenoguanine + alpha-D-ribose 1-phosphate
1,N2-ethenoguanosine + phosphate
-
-
-
r
1,N6-etheno-isoguanine + alpha-D-ribose 1-phosphate
1,N6-etheno-isoguanosine + phosphate
isoguanine is 2-hydroxy-6-aminopurine and showing a solvent-induced keto-enol tautomerism
-
-
r
1,N6-ethenoadenine + alpha-D-ribose 1-phosphate
1,N6-ethenoadenine N7-riboside + phosphate
-
-
-
r
1,N6-ethenoadenine + alpha-D-ribose 1-phosphate
1,N6-ethenoadenine N9-riboside + phosphate
-
-
-
r
1,N6-ethenoadenosine + phosphate
1,N6-ethenoadenine + beta-D-ribose 1-phosphate
i.e. 3-beta-D-ribosylimidazo[2,l-i]purine
-
-
r
1-methyladenosine + phosphate
1-methyladenine + alpha-D-ribose 1-phosphate
-
-
-
r
1-methylguanosine + phosphate
1-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
r
1-methylguanosine + phosphate
alpha-D-ribose 1-phosphate + 1-methylguanine
-
-
-
-
?
1-methylinosine + phosphate
alpha-D-ribose 1-phosphate + 1-methylhypoxanthine
-
-
-
-
?
2',3'-dideoxyinosine + phosphate
hypoxanthine + 2,3-dideoxy-alpha-D-ribose 1-phosphate
-
-
-
?
2',3'-dideoxyinosine + phosphate
hypoxanthine + alpha-D-2,3-dideoxyribose 1-phosphate
-
-
-
-
?
2'-amino-2'-deoxyadenosine + phosphate
adenine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate
2'-amino-2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate
-
-
-
?
2'-deoxy-2'-fluoroadenosine + phosphate
adenine + 2-deoxy-2-fluoro-alpha-D-arabinofuranose 1-phosphate
-
-
-
?
2'-deoxyadenosine + phosphate
2-deoxy-alpha-D-ribose 1-phosphate + adenine
-
10% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + alpha-D-2-deoxyribose 1-phosphate
-
-
-
-
?
2'-deoxyinosine + phosphate
2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine
-
-
-
-
?
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N7-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N8-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N8-ribosyl-2,6-diamino-8-azapurine + N7-ribosyl-2,6-diamino-8-azapurine + phosphate
-
-
-
-
?
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N9-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diaminopurine + alpha-D-ribose 1-phosphate
2,6-diaminopurine ribonucleoside + phosphate
-
-
-
r
2,6-diaminopurine nucleoside + phosphate
alpha-D-ribose 1-phosphate + 2,6-diaminopurine
-
-
-
?
2,6-diaminopurine ribonucleoside + phosphate
2,6-diaminopurine + alpha-D-ribose 1-phosphate
-
-
-
-
?
2-amino-6-chloropurine + alpha-D-ribose 1-phosphate
2-amino-6-chloropurine ribonucleoside + phosphate
-
-
-
r
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + ?
-
-
-
-
?
2-chloroadenosine + phosphate
2-chloroadenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
2-fluoro-2-deoxyadenosine + phosphate
adenine + 2-fluoro-2-deoxy-alpha-D-ribose 1-phosphate
2-fluoroadenosine + phosphate
2-fluoroadenine + alpha-D-ribose 1-phosphate
-
-
-
?
5'-chloro-5'-deoxy-adenosine + phosphate
5-chloro-5-deoxy-D-ribose 1-phosphate + adenine
-
-
-
?
5'-deoxy-5'-fluoroadenosine + phosphate
5-deoxy-5-fluoro-D-ribose-1-phosphate + adenine
-
5'-deoxy-5'-fluoroadenosine + phosphate i.e. 5'-FDA
5-deoxy-5-fluoro-D-ribose-1-phosphate i.e. 5-FDRP
-
?
5'-deoxy-5'-iodo-2-fluoroadenosine + phosphate
2-fluoroadenine + 5-deoxy-5-iodo-alpha-D-ribose 1-phosphate
-
-
-
-
?
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-deoxy-5-methylthio-alpha-D-ribose 1-phosphate
6-(2-thienyl)-9-(beta-D-ribofuranosyl)purine + phosphate
6-(2-thienyl)purine + alpha-D-ribose 1-phosphate
-
-
-
-
?
6-cyclopropyl-9-(beta-D-ribofuranosyl)purine + phosphate
6-cyclopropylpurine + alpha-D-ribose 1-phosphate
-
-
-
-
?
6-ethyl-9-(beta-D-ribofuranosyl)purine + phosphate
6-ethylpurine + alpha-D-ribose 1-phosphate
-
-
-
-
?
6-mercaptoguanosine + phosphate
alpha-D-ribose 1-phosphate + 6-mercaptoguanine
-
-
-
-
?
6-mercaptopurine-2'-deoxyriboside + phosphate
6-mercaptopurine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
r
6-phenyl-9-(beta-D-ribofuranosyl)purine + phosphate
6-phenylpurine + alpha-D-ribose 1-phosphate
-
-
-
-
?
6-thioguanine + alpha-D-ribose 1-phosphate
6-thioguanine ribonucleoside + phosphate
-
-
-
r
7,8-dimethylguanosine + phosphate
7,8-dimethylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-butylguanosine + phosphate
7-butylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-ethylguanosine + phosphate
7-ethylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-isobutylguanosine + phosphate
7-isobutylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-isopropylguanosine + phosphate
7-isopropylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methyl-6-thio-guanosine + phosphate
7-methyl-6-thioguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methyl-6-thioguanosine + phosphate
7-methyl-6-thioguanine + alpha-D-ribose 1-phosphate
-
-
-
r
7-methyl-8-aminoguanosine + phosphate
7-methyl-8-aminoguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylguanine + alpha-D-ribose 1-phosphate
7-methylguanosine + phosphate
-
-
-
r
7-prolylguanosine + phosphate
7-prolylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
8-azaadenine + alpha-D-ribose 1-phosphate
8-azaadenosine + phosphate
-
-
-
r
8-azaguanine + alpha-D-ribose 1-phosphate
8-azaguanine N7-D-riboside + phosphate
-
-
-
r
8-azaguanine + alpha-D-ribose 1-phosphate
N9-beta-D-ribosyl-8-azaguanine + phosphate
-
-
-
-
?
8-azahypoxanthine + alpha-D-ribose 1-phosphate
8-azainosine + phosphate
-
-
?
8-methylguanosine + phosphate
8-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
9-(2-deoxy-2-fluoro-alpha-D-arabinofuranosyl)adenine + phosphate
adenine + 2-deoxy-2-fluoro-alpha-D-arabinofuranose 1-phosphate
-
-
-
?
9-(6-deoxy-5-C-methyl-beta-D-ribo-hexofuranosyl)-6-methylpurine + phosphate
?
-
-
-
-
r
9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine + phosphate
6-methylthiopurine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
9-beta-D-arabinosyl-2-fluoroadenine + phosphate
2-fluoroadenine + beta-D-arabinose 1-phosphate
i.e. fludarabine
-
-
r
9-beta-D-ribofuranosyl-6-methylthiopurine + phosphate
6-methylthiopurine + alpha-D-ribose 1-phosphate
-
-
-
?
9-[beta-D-arabinofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug
-
-
?
adenine arabinoside + phosphate
alpha-D-arabinose 1-phosphate + adenine
-
49% of the activity with adenosine
-
-
?
adenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
activity with mutant enzyme N239D, wild-type and mutant enzyme Y191L show no activity
-
-
?
arabinofuranosyladenine + phosphate
?
4.3% of the activity with inosine
-
-
?
cytokinin + phosphate
? + alpha-D-ribose 1-phosphate
-
the enzyme prefers cytokinin to unsubstituted aminopurines
-
-
r
dihydrozeatin riboside + phosphate
dihydrozeatin + alpha-D-ribose 1-phosphate
-
-
-
-
r
isopentenyladenosine + phosphate
isopentenyladenine + alpha-D-ribose 1-phosphate
-
-
-
-
r
N2,3-etheno-O6-methylguanine + alpha-D-ribose 1-phosphate
N2,3-etheno-O6-methylguanosine + phosphate
-
-
-
r
N2,3-ethenoguanine + alpha-D-ribose 1-phosphate
N2,3-ethenoguanosine + phosphate
-
-
-
r
N7-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N8-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N9-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
O6-methyl-N2,3-ethenoguanine + alpha-D-ribose 1-phosphate
O6-methyl-N2,3-ethenoguanosine + phosphate
-
-
-
r
ribavirin + phosphate
1,2,4-triazole-3-carboxamide + alpha-D-ribose 1-phosphate
-
-
-
-
?
ribavirin + phosphate
alpha-D-ribose 1-phosphate + 1,2,4-triazole-3-carboxamide
-
22% of the activity with inosine. Phosphorolysis reaches an equilibrium when 15% of ribavirin is phosphorolyzed
-
-
r
trans-zeatin riboside + phosphate
trans-zeatin + alpha-D-ribose 1-phosphate
-
-
-
-
r
2',3'-dideoxyinosine + phosphate
alpha-D-2,3-dideoxyribose 1-phosphate + hypoxanthine
-
22% of the activity with inosine
-
-
?
2',3'-dideoxyinosine + phosphate
alpha-D-2,3-dideoxyribose 1-phosphate + hypoxanthine
-
12% of the activity with inosine
-
-
?
2'-amino-2'-deoxyadenosine + phosphate
adenine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate
-
-
-
?
2'-amino-2'-deoxyadenosine + phosphate
adenine + 2-deoxy-2-imino-alpha-D-ribose 1-phosphate
-
-
-
?
2'-deoxy-6-methylpurine nucleoside + phosphate
?
13.2% of the activity with inosine
-
-
?
2'-deoxy-6-methylpurine nucleoside + phosphate
?
13.2% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 15% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 15% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
12% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
54.7% of the activity with 2'-deoxyinosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
13.4% of the activity with inosine
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-alpha-D-ribose 1-phosphate
-
37% activity compared to adenosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
moderate activity
-
-
r
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 17% of the activity with inosine, isoform of 702 amino acids, 18% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 17% of the activity with inosine, isoform of 702 amino acids, 18% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
20% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
83.2% of the activity with 2'-deoxyinosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
3.7% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
3.7% of the activity with inosine
-
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
-
22.5% phosphorolysis of substrate within 30 min
-
?
2'-deoxyguanosine + phosphate
guanine + 2-deoxy-alpha-D-ribose 1-phosphate
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
22.5% phosphorolysis of substrate within 30 min
-
?
2'-deoxyinosine + phosphate
alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
-
-
-
?
2'-deoxyinosine + phosphate
alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
-
-
-
-
?
2'-deoxyinosine + phosphate
alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
-
-
-
-
?
2'-deoxyinosine + phosphate
alpha-D-2-deoxyribose 1-phosphate + hypoxanthine
-
-
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 23% of the activity with inosine, isoform of 702 amino acids, 31% of the activity with inosine
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
22% of the activity with inosine
-
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
40.5% phosphorolysis of substrate within 30 min
-
?
2'-deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
40.5% phosphorolysis of substrate within 30 min
-
?
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N7-D-ribosyl-2,6-diamino-8-azapurine + phosphate
enzyme PNP mutant D204N and mutant N243D
-
-
r
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N7-D-ribosyl-2,6-diamino-8-azapurine + phosphate
PNP mutant D204N
-
-
r
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N7-D-ribosyl-2,6-diamino-8-azapurine + phosphate
enzyme PNP mutant D204N
-
-
r
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N8-D-ribosyl-2,6-diamino-8-azapurine + phosphate
-
-
-
r
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N8-D-ribosyl-2,6-diamino-8-azapurine + phosphate
wild-type enzyme PNP
-
-
r
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N8-D-ribosyl-2,6-diamino-8-azapurine + phosphate
wild-type enzyme PNP
-
-
r
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N9-D-ribosyl-2,6-diamino-8-azapurine + phosphate
enzyme PNP mutant N243D
-
-
r
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N9-D-ribosyl-2,6-diamino-8-azapurine + phosphate
PNP mutant D204N
-
-
r
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
N9-D-ribosyl-2,6-diamino-8-azapurine + phosphate
enzyme PNP mutant D204N
-
-
r
2-amino-6-mercapto-7-methylpurine ribonucleoside + alpha-D-ribose 1-phosphate
?
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + alpha-D-ribose 1-phosphate
?
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + alpha-D-ribose 1-phosphate
?
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
-
-
-
-
?
2-amino-6-mercapto-7-methylpurine ribonucleoside + phosphate
alpha-D-ribose 1-phosphate + 2-amino-6-mercapto-7-methylpurine
-
-
-
-
?
2-fluoro-2-deoxyadenosine + phosphate
adenine + 2-fluoro-2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
2-fluoro-2-deoxyadenosine + phosphate
adenine + 2-fluoro-2-deoxy-alpha-D-ribose 1-phosphate
-
no substrate for wild-type, substrate of double mutant E201Q/N243D
-
-
?
2-fluoro-2-deoxyadenosine + phosphate
adenine + 2-fluoro-2-deoxy-alpha-D-ribose 1-phosphate
-
-
the product 2-fluoroadenine is highly cytotoxic for Trichomonas vaginalis
-
?
3-(beta-D-ribofuranosyl)adenine + alpha-D-ribose 1-phosphate
?
-
-
-
?
3-(beta-D-ribofuranosyl)hyopxanthine + alpha-D-ribose 1-phosphate
?
-
-
-
?
3-(beta-D-ribofuranosyl)hyopxanthine + alpha-D-ribose 1-phosphate
?
-
-
-
-
?
3-(beta-D-ribofuranosyl)hyopxanthine + alpha-D-ribose 1-phosphate
?
-
-
-
?
3-deazainosine + phosphate
alpha-D-ribose 1-phosphate + 3-deazahypoxanthine
-
-
-
-
?
3-deazainosine + phosphate
alpha-D-ribose 1-phosphate + 3-deazahypoxanthine
-
-
-
-
?
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-deoxy-5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
?
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-deoxy-5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
?
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-deoxy-5-methylthio-alpha-D-ribose 1-phosphate
-
40% activity compared to adenosine
-
-
?
5'-methylthioadenosine + phosphate
adenine + 5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
?
5'-methylthioadenosine + phosphate
adenine + 5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
r
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
-
-
r
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
ver low activity
-
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
very low activity
-
-
?
6-mercaptopurine riboside + phosphate
alpha-D-ribose 1-phosphate + 6-mercaptopurine
-
-
-
-
?
6-mercaptopurine riboside + phosphate
alpha-D-ribose 1-phosphate + 6-mercaptopurine
-
14% of the activity with inosine
-
-
?
6-thioxanthine + alpha-D-ribose 1-phosphate
6-thioxanthosine + phosphate
-
-
-
?
6-thioxanthine + alpha-D-ribose 1-phosphate
6-thioxanthosine + phosphate
-
-
-
-
?
7-(beta-D-ribofuranosyl)guanine + alpha-D-ribose 1-phosphate
?
-
-
-
?
7-(beta-D-ribofuranosyl)hypoxanthine + alpha-D-ribose 1-phosphate
?
-
-
-
?
7-(beta-D-ribofuranosyl)hypoxanthine + alpha-D-ribose 1-phosphate
?
-
-
-
?
7-(beta-D-ribofuranosyl)hypoxanthine + alpha-D-ribose 1-phosphate
?
-
-
-
?
7-methyladenosine + phosphate
7-methyladenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methyladenosine + phosphate
7-methyladenine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methyladenosine + phosphate
7-methyladenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methyladenosine + phosphate
7-methyladenine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methyladenosine + phosphate
7-methyladenine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
r
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
r
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
low activity
-
-
r
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
r
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
r
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylguanosine + phosphate
7-methylguanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
7-methylinosine + phosphate
7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
-
-
-
-
?
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
-
-
-
?
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
-
-
-
?
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
-
-
-
?
8-aminoguanine + alpha-D-ribose 1-phosphate
8-aminoguanosine + phosphate
-
-
-
?
8-azaguanine + alpha-D-ribose 1-phosphate
8-azaguanosine + phosphate
-
-
-
-
?
8-azaguanine + alpha-D-ribose 1-phosphate
8-azaguanosine + phosphate
-
-
-
?
8-azaguanine + alpha-D-ribose 1-phosphate
8-azaguanosine + phosphate
-
-
-
r
8-azaguanine + alpha-D-ribose 1-phosphate
8-azaguanosine + phosphate
-
-
-
r
8-azaguanosine + phosphate
8-azaguanine + alpha-D-ribose 1-phosphate
-
-
-
r
8-azaguanosine + phosphate
8-azaguanine + alpha-D-ribose 1-phosphate
-
-
-
r
9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine + phosphate
?
-
a toxic prodrug
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-6-methylpurine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V, liberation of methylpurine
-
-
?
9-[beta-L-lyxofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V, the drug product has anti-tumor activity
-
-
?
9-[beta-L-lyxofuranosyl]-2-fluoro-adenine + phosphate
?
-
a toxic prodrug, substrate of enzyme mutant M64V
-
-
?
adenine + deoxyribose 1-phosphate
deoxyadenosine + phosphate
-
-
-
-
?
adenosine + arsenate
adenine + alpha-D-ribose 1-arsenate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
low activity
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
reaction with adenosine-specific phosphorylase, no activity with inosine-guanosine phosphorylase
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 48% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 1% of the activity with inosine, isoform of 702 amino acids, 48% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
no activity
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
no activity
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
61% of the activity with deoxyguanosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
41% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
59.9% of the activity with 2'-deoxyinosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
no activity
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
160% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
activity with OUNOII, no activity with PUNPI
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
160% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
no activity
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
very low activity
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
low activity
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
low activity
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
low activity
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
very low activity
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
best substrate
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
best substrate
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
wild-type enzyme and mutant enzyme K244Q show no activity with adenosine, mutant enzyme N243D and N243D/K244Q are active with adenosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
48% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
48% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
15.4% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
45% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
15.4% of the activity with inosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
78% of the activity with deoxyguanosine
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
100% activity
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
exclusive substrate for the hexameric enzyme
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
-
-
r
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
exclusive substrate for the hexameric enzyme
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
-
in the reverse reaction the catalytic activity with adenine is higher than that with either hypoxanthine or guanine
-
-
r
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
reaction with adenosine-specific phosphorylase, no activity with inosine-guanosine phosphorylase
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
112.9% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
112.9% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
61% of the activity with deoxyguanosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
2'-deoxyadenosine, 160% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
2'-deoxyadenosine, 65% of the activity with adenosine. 3'-deoxyadenosine, 8% of the activity with adenosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
2'-deoxyadenosine, 65% of the activity with adenosine. 3'-deoxyadenosine, 8% of the activity with adenosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
2'-deoxyadenosine, 57% of the activity with inosine. 3'-deoxyadenosine, 7% of the activity with inosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
78% of the activity with deoxyguanosine
-
-
?
deoxyadenosine + phosphate
alpha-D-ribose 1-phosphate + adenine
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
reaction with ionosine-guanosine phosphorylase, very low activity with the adenosine-specific phosphorylase
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
11% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyguanosine, 469% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
85% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyguanosine, 469% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
85% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyguanosine, 41% of the activity with inosine. 3'-deoxyguanosine, 16% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyguanosine, 41% of the activity with inosine. 3'-deoxyguanosine, 16% of the activity with inosine
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyguanosine + phosphate
guanine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
14% of the activity with inosine
-
-
?
deoxyinosine + phosphate
hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate
-
19% of the activity with inosine
-
-
?
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
reaction with inosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyinosine and 5'-deoxyinosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
88% of the activity with deoxyguanosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyinosine, 87% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
as active as inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyinosine, 691% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyinosine, 691% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
as active as inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyinosine, 43% of the activity with adenosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
2'-deoxyinosine, 43% of the activity with adenosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
136% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
136% of the activity with inosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
?
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
?
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
88% of the activity with deoxyguanosine
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
-
r
deoxyinosine + phosphate
hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
-
-
?
guanosine + arsenate
guanine + alpha-D-ribose 1-arsenate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
no activity
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
moderate activity
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
moderate activity
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
reaction with inosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 87% of the activity with inosine, isoform of 702 amino acids, 46% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
isoform of 816 amino acids, 87% of the activity with inosine, isoform of 702 amino acids, 46% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
47.4% of the reaction with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
47.4% of the reaction with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
48% of the activity with deoxyguanosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
no activity with mutant enzyme N239D
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
58.7% of the activity with 2'-deoxyinosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
66% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
21% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
53% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
84% of the activity with guanosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
53% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
84% of the activity with guanosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
Halalkalibacterium halodurans
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
Halalkalibacterium halodurans Alk36
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
insight into catalytically relevant correlated motions in human purine nucleoside phosphorylase
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
2'-deoxyinosine, 27% of the activity with inosine. 3'-deoxyinosine, 10% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
2'-deoxyinosine, 27% of the activity with inosine. 3'-deoxyinosine, 10% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
2.3% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
55.8% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
56% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
55.8% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
absolute specificity for inosine and guanosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
16% of the activity with inosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
81% of the activity with deoxyguanosine
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
8.7% phosphorolysis of substrate within 30 min
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
exclusive substrate for the trimeric enzyme
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
8.7% phosphorolysis of substrate within 30 min
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
exclusive substrate for the trimeric enzyme
-
-
?
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + arsenate
hypoxanthine + ribose 1-arsenate
-
ribosyl group is nearly dissociated from the base prior to attack of the arsenate
-
?
inosine + arsenate
hypoxanthine + ribose 1-arsenate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
no activity
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
best substrate
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
best substrate
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
reaction with ionosine-guanosine phosphorylase, no activity with the adenosine-specific phosphorylase
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
the equilibrium is reached when approximately 14% of inosine is phosphorylated
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
the equilibrium is reached when approximately 14% of inosine is phosphorylated
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
highest activity
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
46% of the activity with deoxyguanosine
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
83% of the activity with 2'-deoxyinosine
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
reaction reaches an equilibrium when 21% of the inosine is phosphorolyzed
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
Halalkalibacterium halodurans
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
Halalkalibacterium halodurans Alk36
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
best substrate
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
best substrate
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
best substrate
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
mechanism
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
complete conversion
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
34% of the activity with adenosine
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
equilibrium when 65% of inosine has been phosphorylated
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
34% of the activity with adenosine
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
equilibrium when 65% of inosine has been phosphorylated
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
preferred substrate
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
preferred substrate
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
preferred substrate
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
preferred substrate
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
preferred substrate
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
preferred substrate
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
preferred substrate
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
complete conversion
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
absolute specificity for inosine and guanosine
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
82% of the activity with deoxyguanosine
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
19.1% phosphorolysis of substrate within 30 min
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
19.1% phosphorolysis of substrate within 30 min
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
r
methylthioinosine + phosphate
methylthiohypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
methylthioinosine + phosphate
methylthiohypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
methylthioinosine + phosphate
methylthiohypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
-
?
N7-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
the N7-riboside of 8-azaDaPur seems to be quite rapidly and specifically phosphorolysed by calf PNP. The mutation at Asn243 markedly accelerates this process
-
-
r
N7-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
enzyme PNP mutant D204N
-
-
r
N7-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
-
-
-
r
N8-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
wild-type enzyme PNP
-
-
r
N8-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
wild-type enzyme PNP
-
-
r
N9-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
enzyme PNP mutant N243D
-
-
r
N9-D-ribosyl-2,6-diamino-8-azapurine + phosphate
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
enzyme PNP mutant D204N
-
-
r
nicotinamide riboside + phosphate
nicotinamide + alpha-D-ribose 1-phosphate
-
-
-
?
nicotinamide riboside + phosphate
nicotinamide + alpha-D-ribose 1-phosphate
-
-
-
?
nicotinamide riboside + phosphate
nicotinamide + alpha-D-ribose 1-phosphate
-
-
-
r
nicotinamide riboside + phosphate
nicotinamide + alpha-D-ribose 1-phosphate
-
-
-
?
nicotinamide riboside + phosphate
nicotinamide + alpha-D-ribose 1-phosphate
-
-
-
r
purine deoxyribonucleoside + phosphate
purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
purine deoxyribonucleoside + phosphate
purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
purine deoxyribonucleoside + phosphate
purine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
purine ribonucleoside + phosphate
purine + alpha-D-ribose 1-phosphate
-
-
-
?
purine ribonucleoside + phosphate
purine + alpha-D-ribose 1-phosphate
-
-
-
-
?
purine ribonucleoside + phosphate
purine + alpha-D-ribose 1-phosphate
-
-
-
-
?
purine ribonucleoside + phosphate
purine + alpha-D-ribose 1-phosphate
-
-
-
r
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
-
43.5% of the activity with inosine
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
-
43.5% of the activity with inosine
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
no activity with mutant enzymes Y191L and N239D
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
-
26% of the activity with inosine
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
-
-
-
-
?
xanthosine + phosphate
alpha-D-ribose 1-phosphate + xanthine
-
reaction only at high concentrations
-
-
?
xanthosine + phosphate
xanthine + alpha-D-ribose 1-phosphate
-
-
-
r
xanthosine + phosphate
xanthine + alpha-D-ribose 1-phosphate
-
-
-
r
additional information
?
-
the enzyme cannot accept 6-chloropurines
-
-
?
additional information
?
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
the enzyme cannot accept 6-chloropurines
-
-
?
additional information
?
-
substrate specificity, detailed overview, no activity with 2'-deoxyadenosine
-
-
-
additional information
?
-
-
substrate specificity, detailed overview, no activity with 2'-deoxyadenosine
-
-
-
additional information
?
-
substrate specificity, detailed overview, no activity with 2'-deoxyadenosine
-
-
-
additional information
?
-
no activity is detected for adenosine or 2'-deoxyadenosine
-
-
?
additional information
?
-
-
no activity is detected for adenosine or 2'-deoxyadenosine
-
-
?
additional information
?
-
-
two purine nucleoside phosphorylases: 1. inosine-guanosine phosphorylase, 2. adenosine-specific phosphorylase
-
-
?
additional information
?
-
N7-D-ribosyl-2,6-diamino-8-azapurine is a good fluorogenic substrate for mammalian forms of PNP, while the N8-riboside is selective to the Escherichia coli enzyme. The altered specificity of the PNP mutants towards some of the purine analogues can be related to differences in binding modes of 8-azapurine bases in the PNP active site, including binding in the upside-down position (rotation by 180 degree when compared with standard binding mode). Ribosylation of 2,6-diamino-8-azapurine leads to at least three fluorescent ribosides. The N9-riboside is very highly fluorescent, but its fluorescence is generally similar, in terms of lambdamax and decay time, to that of the free base. By contrast, fluorescence of N7- and N8-ribosides is red-shifted by about 70 nm and thanks to this shift their cleavage via phosphorolysis (or hydrolysis) leads to a high fluorogenic effect at about 360 nm, which can be analytically useful
-
-
-
additional information
?
-
the trimeric PNP binds adenosine but it does not catalyze its phosphorolysis
-
-
-
additional information
?
-
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
catalysis involves protonation of the purine base at position N7 by residue Asp204, which is triggered by Arg217
-
-
?
additional information
?
-
-
catalysis involves protonation of the purine base at position N7 by residue Asp204, which is triggered by Arg217
-
-
?
additional information
?
-
-
enzyme shows a high tolerance to the steric and hydrophobic environment at the 6-position of the synthesized purine ribonucleosides
-
-
?
additional information
?
-
no substrate: xanthosine, uridine, 2'-deoxythymidine, cytidine
-
-
?
additional information
?
-
-
no substrate: xanthosine, uridine, 2'-deoxythymidine, cytidine
-
-
?
additional information
?
-
etheno-derivatives of guanine, O6-methylguanine, and isoguanine are prepared and examined as potential substrates of purine-nucleoside phosphorylase in the reverse (synthetic) pathway. 1,N2-ethenoguanine and 1,N6-etheno-isoguanine are excellent substrates for purine-nucleoside phosphorylase (PNP) from Escherichia coli, while O6-methyl-N2,3-ethenoguanine exhibits moderate activity with this enzyme. The ribosylation reaction is fully reversible by the addition of a phosphate buffer up to a concentration of about 5 mM. The inability of the enzyme to ribosylate N2,3-ethenoguanine is not due to geometric hindrance, but rather should be ascribed to unfavorable energetic factors, in accordance with previous reports about the relative instability of the glycosidic bond in this compound. N2,3-etheno-O6-methylguanine is a poor substrate, it competitively competes with guanine in the ribosylation process, acting as quasi inhibitor of the Escherichia coli PNP. 6-Aminopurine ribosides, not possessing a mobile proton at the N1 position, do not react with trimeric forms of PNP
-
-
-
additional information
?
-
-
etheno-derivatives of guanine, O6-methylguanine, and isoguanine are prepared and examined as potential substrates of purine-nucleoside phosphorylase in the reverse (synthetic) pathway. 1,N2-ethenoguanine and 1,N6-etheno-isoguanine are excellent substrates for purine-nucleoside phosphorylase (PNP) from Escherichia coli, while O6-methyl-N2,3-ethenoguanine exhibits moderate activity with this enzyme. The ribosylation reaction is fully reversible by the addition of a phosphate buffer up to a concentration of about 5 mM. The inability of the enzyme to ribosylate N2,3-ethenoguanine is not due to geometric hindrance, but rather should be ascribed to unfavorable energetic factors, in accordance with previous reports about the relative instability of the glycosidic bond in this compound. N2,3-etheno-O6-methylguanine is a poor substrate, it competitively competes with guanine in the ribosylation process, acting as quasi inhibitor of the Escherichia coli PNP. 6-Aminopurine ribosides, not possessing a mobile proton at the N1 position, do not react with trimeric forms of PNP
-
-
-
additional information
?
-
hexameric PNPs accept as substrates many nucleoside analogues with various modifications in the purine ring, with an important exception of 7-deaza nucleosides. N7-Methylated guanosine, inosine and adenosine are unusual fluorescent substrates of both trimeric and hexameric PNPs
-
-
-
additional information
?
-
hexameric PNPs accept as substrates many nucleoside analogues with various modifications in the purine ring, with an important exception of 7-deaza nucleosides. N7-Methylated guanosine, inosine and adenosine are unusual fluorescent substrates of both trimeric and hexameric PNPs
-
-
-
additional information
?
-
N1-methylated guanosine, inosine, and adenosine derivatives are selective substrates for hexameric PNP from Escherichia coli. Hexameric PNPs accept as substrates many nucleoside analogues with various modifications in the purine ring, with an important exception of 7-deaza nucleosides. N7-Methylated guanosine, inosine and adenosine are unusual fluorescent substrates of both trimeric and hexameric PNPs. Escherichia coli PNP converts pro-drugs, which are relative nontoxic purine nucleosides (for example 6-methyl purine 2'-deoxynucleoside or fludarabine), to their respective purine analogs (here, to 6-methylpurine and 2-fluoroadenine, respectively), which are very potent, toxic drugs. Enzymatic ribosylation of 8-azapurines, overview. Isoadenosine (3-beta-D-ribosyl-adenine), is, unlike the parent adenosine, a quite good substrate for mammalian and bacterial PNP. Ribosylation of tri-cyclic nucleobase analogues and phosphorolysis
-
-
-
additional information
?
-
N1-methylated guanosine, inosine, and adenosine derivatives are selective substrates for hexameric PNP from Escherichia coli. Hexameric PNPs accept as substrates many nucleoside analogues with various modifications in the purine ring, with an important exception of 7-deaza nucleosides. N7-Methylated guanosine, inosine and adenosine are unusual fluorescent substrates of both trimeric and hexameric PNPs. Escherichia coli PNP converts pro-drugs, which are relative nontoxic purine nucleosides (for example 6-methyl purine 2'-deoxynucleoside or fludarabine), to their respective purine analogs (here, to 6-methylpurine and 2-fluoroadenine, respectively), which are very potent, toxic drugs. Enzymatic ribosylation of 8-azapurines, overview. Isoadenosine (3-beta-D-ribosyl-adenine), is, unlike the parent adenosine, a quite good substrate for mammalian and bacterial PNP. Ribosylation of tri-cyclic nucleobase analogues and phosphorolysis
-
-
-
additional information
?
-
N7-D-ribosyl-2,6-diamino-8-azapurine is a good fluorogenic substrate for mammalian forms of PNP, including human blood PNP, while the N8-riboside is selective to the Escherichia coli enzyme. Wild-type calf PNP gives N7- and N8-ribosides, while the calf enzyme N243D PNP mutant directs the ribosyl substitution at N9- and N7-positions. The Escherichia coli enzyme ribosylates adenine and 8-azaadenine. In both cases, the N9-ribosides are the only detectable ribosylation products for the wild-type enzyme, while the D204N mutant reacting with the latter substrate does produce the N8-riboside. The altered specificity of the PNP mutants towards some of the purine analogues can be related to differences in binding modes of 8-azapurine bases in the PNP active site, including binding in the upside-down position (rotation by 180 degree when compared with standard binding mode). Ribosylation of 2,6-diamino-8-azapurine leads to at least three fluorescent ribosides. The N9-riboside is very highly fluorescent, but its fluorescence is generally similar, in terms of lambdamax and decay time, to that of the free base. By contrast, fluorescence of N7- and N8-ribosides is red-shifted by about 70 nm and thanks to this shift their cleavage via phosphorolysis (or hydrolysis) leads to a high fluorogenic effect at about 360 nm, which can be analytically useful
-
-
-
additional information
?
-
-
N7-D-ribosyl-2,6-diamino-8-azapurine is a good fluorogenic substrate for mammalian forms of PNP, including human blood PNP, while the N8-riboside is selective to the Escherichia coli enzyme. Wild-type calf PNP gives N7- and N8-ribosides, while the calf enzyme N243D PNP mutant directs the ribosyl substitution at N9- and N7-positions. The Escherichia coli enzyme ribosylates adenine and 8-azaadenine. In both cases, the N9-ribosides are the only detectable ribosylation products for the wild-type enzyme, while the D204N mutant reacting with the latter substrate does produce the N8-riboside. The altered specificity of the PNP mutants towards some of the purine analogues can be related to differences in binding modes of 8-azapurine bases in the PNP active site, including binding in the upside-down position (rotation by 180 degree when compared with standard binding mode). Ribosylation of 2,6-diamino-8-azapurine leads to at least three fluorescent ribosides. The N9-riboside is very highly fluorescent, but its fluorescence is generally similar, in terms of lambdamax and decay time, to that of the free base. By contrast, fluorescence of N7- and N8-ribosides is red-shifted by about 70 nm and thanks to this shift their cleavage via phosphorolysis (or hydrolysis) leads to a high fluorogenic effect at about 360 nm, which can be analytically useful
-
-
-
additional information
?
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
-
cellular function seems concerned primarily with nucleoside breakdown
-
-
?
additional information
?
-
-
children lacking PNP activity exhibit severe T cell immunodeficiency while maintaining normal or exaggerated B cell function. The PNP deficiency results in very low uric acid concentrations and high concentrations of the nucleoside substrate of PNP in plasma and urine
-
-
?
additional information
?
-
in intact cells, the enzyme functions in the direction of phosphorolysis, leading to degradation of purine nucleosides via coupling with guanase and xanthine oxidase. PNP deficiency is a rare disorder associated with an autosomal recessive form of cellular, but not humoral, immunodeficiency, and comprises about 4% of all cases of severe combined immunodeficiency. Mutations identified in cases of PNP deficiency
-
-
?
additional information
?
-
-
in intact cells, the enzyme functions in the direction of phosphorolysis, leading to degradation of purine nucleosides via coupling with guanase and xanthine oxidase. PNP deficiency is a rare disorder associated with an autosomal recessive form of cellular, but not humoral, immunodeficiency, and comprises about 4% of all cases of severe combined immunodeficiency. Mutations identified in cases of PNP deficiency
-
-
?
additional information
?
-
enzyme plays a key role in the purine salvage pathway. PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function
-
-
?
additional information
?
-
-
enzyme plays a key role in the purine salvage pathway. PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function
-
-
?
additional information
?
-
N7-D-ribosyl-2,6-diamino-8-azapurine is a good fluorogenic substrate for mammalian forms of PNP, while the N8-riboside is selective to the Escherichia coli enzyme. Ribosylation of 2,6-diamino-8-azapurine leads to at least three fluorescent ribosides. The N9-riboside is very highly fluorescent, but its fluorescence is generally similar, in terms of lambdamax and decay time, to that of the free base. By contrast, fluorescence of N7- and N8-ribosides is red-shifted by about 70 nm and thanks to this shift their cleavage via phosphorolysis (or hydrolysis) leads to a high fluorogenic effect at about 360 nm, which can be analytically useful
-
-
-
additional information
?
-
-
N7-D-ribosyl-2,6-diamino-8-azapurine is a good fluorogenic substrate for mammalian forms of PNP, while the N8-riboside is selective to the Escherichia coli enzyme. Ribosylation of 2,6-diamino-8-azapurine leads to at least three fluorescent ribosides. The N9-riboside is very highly fluorescent, but its fluorescence is generally similar, in terms of lambdamax and decay time, to that of the free base. By contrast, fluorescence of N7- and N8-ribosides is red-shifted by about 70 nm and thanks to this shift their cleavage via phosphorolysis (or hydrolysis) leads to a high fluorogenic effect at about 360 nm, which can be analytically useful
-
-
-
additional information
?
-
nicotinamide riboside is a substrate of trimeric PNPs because it mimics 6-keto/N1H arrangement of 6-oxopurine nucleosides and 8-azaguanine. For trimeric PNP, the proton at the purine position N1 is required for catalysis. N7-Methylated guanosine, inosine and adenosine are unusual fluorescent substrates of both trimeric and hexameric PNPs. No activity with pro-drugs 6-methyl purine 2'-deoxynucleoside or fludarabine (9-beta-D-arabinosyl-2-fluoroadenine). Enzymatic ribosylation of 8-azapurines, overview. Isoadenosine (3-beta-D-ribosyl-adenine), is, unlike the parent adenosine, a quite good substrate for mammalian and bacterial PNP. Ribosylation of tri-cyclic nucleobase analogues and phosphorolysis
-
-
-
additional information
?
-
the nutraceutical nicotinamide riboside (NR), an efficacious biosynthetic precursor to NAD, is readily metabolized by the purine nucleoside phosphorylase (PNP). Access to the PNP-stable versions of NR is difficult because the glycosidic bond of NR is easily cleaved. Unlike NR, NRH, the reduced form of NR, offers sufficient chemical stability to allow the successful functionalisation of the ribosyl-moiety. A series of NRH and NR derived amino acid conjugates is generated in good to excellent yields and show that O5'-esterification prevents the PNP-catalyzed phosphorolysis of these NR prodrugs, overview. Enzyme PNP catalyzes the phosphorolysis of nicotinamide riboside chloride (NR-Cl) and glycine nicotinamide riboside conjugate (Gly-NR)
-
-
-
additional information
?
-
the recombinant enzyme does not metabolize adenosine to adenine or N6-etheno-adenosine to N6-etheno-adenine
-
-
-
additional information
?
-
no significant activity towards adenosine. Substrate binding structure analysis, active site structure comparisons, overview
-
-
-
additional information
?
-
-
no significant activity towards adenosine. Substrate binding structure analysis, active site structure comparisons, overview
-
-
-
additional information
?
-
no significant activity towards adenosine. Substrate binding structure analysis, active site structure comparisons, overview
-
-
-
additional information
?
-
no significant activity towards adenosine. Substrate binding structure analysis, active site structure comparisons, overview
-
-
-
additional information
?
-
no significant activity towards adenosine. Substrate binding structure analysis, active site structure comparisons, overview
-
-
-
additional information
?
-
no significant activity towards adenosine. Substrate binding structure analysis, active site structure comparisons, overview
-
-
-
additional information
?
-
no significant activity towards adenosine. Substrate binding structure analysis, active site structure comparisons, overview
-
-
-
additional information
?
-
no significant activity towards adenosine. Substrate binding structure analysis, active site structure comparisons, overview
-
-
-
additional information
?
-
Leucisus rusticus
-
in fish skin the enzyme plays a key role in the deposition of guanine and hypoxanthine crystals
-
-
?
additional information
?
-
-
enzyme is more specific to natural 6-oxopurine nucleosides and synthetic compounds, and does not catalyze the phosphorolysis of adenosine
-
-
?
additional information
?
-
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
the complete hydrophobic pocket plays an important role in the catalytic function. A potential transition state structure is present in hydrogen bond between the carboxyl groups of Thr90 in the phosphate binding site
-
-
?
additional information
?
-
-
the complete hydrophobic pocket plays an important role in the catalytic function. A potential transition state structure is present in hydrogen bond between the carboxyl groups of Thr90 in the phosphate binding site
-
-
?
additional information
?
-
the complete hydrophobic pocket plays an important role in the catalytic function. A potential transition state structure is present in hydrogen bond between the carboxyl groups of Thr90 in the phosphate binding site
-
-
?
additional information
?
-
-
enzyme is able to reactivate scrambled RNaseA
-
-
?
additional information
?
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
-
-
-
additional information
?
-
in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine
-
-
-
additional information
?
-
in cultured cells (CFs, GMCs, PGVSMCs), isolated perfused kidneys, and in vivo, N6-etheno-adenosine is converted to N6-etheno-adenine. This reaction is abolished by 8-aminoguanine. When kidneys are not inserted into the perfusion system, N6-etheno-ATP is not metabolized. The conversions are inhibited by the PNPase inhibitor forodesine
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additional information
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salmon
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in fish skin the enzyme plays a key role in the deposition of guanine and hypoxanthine crystals
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additional information
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the enzyme is specific for adenine nucleosides which includes adenosine analogs modified in the aglycone, pentose or both moieties
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additional information
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no activity with inosine and guanosine
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additional information
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the enzyme has no detectable ribohydrolase activity
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additional information
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no substrate: acyclovir, benzimidazole, 2,6-diaminopurine
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additional information
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no substrate: acyclovir, benzimidazole, 2,6-diaminopurine
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additional information
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the hexamric PNP binds adenosine but it does not catalyze its phosphorolysis
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additional information
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thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
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additional information
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thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
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additional information
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thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
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additional information
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thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
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additional information
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thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
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additional information
?
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thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview
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additional information
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Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
no substrate: acyclovir, benzimidazole, 2,6-diaminopurine
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