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2.4.2.14: amidophosphoribosyltransferase

This is an abbreviated version!
For detailed information about amidophosphoribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.14

Reaction

5-phospho-beta-D-ribosylamine
+
diphosphate
 +
L-glutamate
=
L-glutamine
 +
5-phospho-alpha-D-ribose 1-diphosphate
 +
H2O

Synonyms

5'-phosphoribosylpyrophosphate amidotransferase, 5-phosphoribosyl-1-pyrophosphate amidotransferase, 5-phosphoribosylpyrophosphate amidotransferase, 5-phosphororibosyl-1-pyrophosphate amidotransferase, ADE4, alpha-5-phosphoribosyl-1-pyrophosphate amidotransferase, amido phosphoribosyltransferase, amidotransferase, phosphoribosyl pyrophosphate, APRT, ATASE, AtGPRT, Gln phosphoribosylpyrophosphate amidotransferase, glutamine 5-phosphoribosylpyrophosphate amidotransferase, glutamine phosphoribosyl pyrophosphate amidotransferase, glutamine phosphoribosylpyrophosphate amidotransferase, glutamine ribosylpyrophosphate 5-phosphate amidotransferase, GPAT, GPATase, GPRAT, GPRAT2, phosphoribose pyrophosphate amidotransferase, phosphoribosyl amidotransferase, phosphoribosyl pyrophosphate amidotransferase, phosphoribosylamidotransferase, phosphoribosyldiphosphate 5-amidotransferase, phosphoribosylpyrophosphate glutamyl amidotransferase, PPAT, PRAT, PurF

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.14 amidophosphoribosyltransferase

Storage Stability

Storage Stability on EC 2.4.2.14 - amidophosphoribosyltransferase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or 4°C, cell-free extract, overnight, more than 50% loss of activity
-
-20°C, crude, more than 50% loss of activity overnight
-
2-mercaptoethanol and phosphoribosyldiphosphate stabilize during storage at 4°C
-
2-mercaptoethanol stabilizes during storage
-
3°C, crude mutant enzyme extract, inactivation overnight, partially purified mutant enzyme: inactivation within 72 h, PMSF stabilizes
-
3°C, crude or partially purified wild-type enzyme, at least 72 h stable
-
4°C, 25% loss of activity per day
-
4°C, 50 mM phosphate bufer, pH 7.4, 5 mM MgCl2, 60 mM 2-mercaptoethanol, at least 4 weeks, no loss of activity
-
4°C, in phosphate buffer, pH 7.4, 60 mM 2-mercaptoethanol and phosphoribosyldiphosphate, at least 10 days
-
4°C, partially purified preparation in 50 mM phosphate buffer, pH 7.4, 5 mM Mg2+ and 60 mM 2-mercaptoethanol, at least 4 weeks
-
4°C, phosphate buffer, 10 d, 40% loss of activity
-
phosphate stabilizes during storage
-
PMSF stabilizes during storage
-
storage in 25 mM Tris buffer inactivates with 26% or 4% residual activity after 6 or 10 days, respectively
-