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2.4.2.18: anthranilate phosphoribosyltransferase

This is an abbreviated version!
For detailed information about anthranilate phosphoribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.18

Reaction

N-(5-phospho-D-ribosyl)-anthranilate
+
diphosphate
=
anthranilate
+
5-phospho-alpha-D-ribose 1-diphosphate

Synonyms

AnPRT, anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase, anthranilate phosphoribosyl transferase, anthranilate phosphoribosylpyrophosphate phosphoribosyltransferase, anthranilate phosphoribosyltransferase, anthranilate PRT, anthranilate-5-phosphoribosylphosphate phosphoribosyltransferase, anthranilate-PP-ribose-P phosphoribosyltransferase, More, Pat, phosphoribosyl-anthranilate pyrophosphorylase, phosphoribosylanthranilate pyrophosphorylase, phosphoribosylanthranilate transferase, phosphoribosyltransferase, anthranilate, PR transferase, PRT, sAnPRT, ssAnPRT, SSO0890, ssTrpD, strpD, Trp D, TrpD, TTC1491

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.18 anthranilate phosphoribosyltransferase

Purification

Purification on EC 2.4.2.18 - anthranilate phosphoribosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
95% pure
anion exchange QFF column chromatography, Resource Q column chromatography and Superdex 75 gel filtration
from Escherichia coli extract by heat-precipitation of host proteins followed by metal chelate affinity chromatography, yield of 0.4-0.8 mg protein per 1 g wet cell mass, >95% purity, stored at -80°C
immobilized metal ion affinity chromatography (Ni2+)
-
Mono Q column chromatography
MonoQ column chromatography
Ni-NTA column chromatography
partial, enzyme complex
-
Strep-Tactin column chromatography
-
wild-type and mutants purified by heat precipitation and metal chelate affinity chromatography