2.4.2.18: anthranilate phosphoribosyltransferase
This is an abbreviated version!
For detailed information about anthranilate phosphoribosyltransferase, go to the full flat file.
Word Map on EC 2.4.2.18
-
2.4.2.18
-
phosphoribosyltransferases
-
chorismate
-
sulfolobus
-
solfataricus
-
phosphorylases
-
indole-3-glycerol
-
glutamine-dependent
-
hafniae
-
4.1.3.27
- 2.4.2.18
-
phosphoribosyltransferases
- chorismate
-
sulfolobus
- solfataricus
- phosphorylases
-
indole-3-glycerol
-
glutamine-dependent
- hafniae
-
4.1.3.27
Reaction
Synonyms
AnPRT, anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase, anthranilate phosphoribosyl transferase, anthranilate phosphoribosylpyrophosphate phosphoribosyltransferase, anthranilate phosphoribosyltransferase, anthranilate PRT, anthranilate-5-phosphoribosylphosphate phosphoribosyltransferase, anthranilate-PP-ribose-P phosphoribosyltransferase, More, Pat, phosphoribosyl-anthranilate pyrophosphorylase, phosphoribosylanthranilate pyrophosphorylase, phosphoribosylanthranilate transferase, phosphoribosyltransferase, anthranilate, PR transferase, PRT, sAnPRT, ssAnPRT, SSO0890, ssTrpD, strpD, Trp D, TrpD, TTC1491
ECTree
2.4.2.18 anthranilate phosphoribosyltransferaseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 2.4.2.18 - anthranilate phosphoribosyltransferase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
46
-
half-life: strain TAX6trpR782: 1.5 min, strain trpAB1653trpR782: 5 min
70
80
half-lives (t1/2) as measure of kinetic stability, t1/2(wild-type): 40 min, t1/2(double mutant M47D/I36E): 3 min, t1/2(mutant I36E, about 1 microM): 3 min, t1/2(mutant I36E, about 20 microM): 40 min, t1/2(mutant M47D, about 1 microM): 4 min, t1/2(mutant M47D, about 47 microM): 15 min, irreversible heat inactivation (pH 6.7) at different time points followed by chilling, centrifugation and estimation of residual activity
82
double mutant I36E/M47D, 12 microM, melting temperature at which half of the protein is unfolded deduced from DSC, pH 7.5
83
mutant M47D, 12 microM, melting temperature at which half of the protein is unfolded deduced from DSC, pH 7.5
85
90
92
wild-type, 12 microM, melting temperature at which half of the protein is unfolded deduced from differential scanning calorimetry (DSC), pH 7.5
additional information
70
t1/2: 69 min, addition of anthranilate has no stabilizing effect
85
t1/2: 35 min, addition of anthranilate has no stabilizing effect
90
mutant I36E, 12 microM, melting temperature at which half of the protein is unfolded deduced from DSC, pH 7.5
90
wild-type protein and mutant D83G show melting temperatures of 90 and 91°C, respectively, mutant F149S protein and the double mutant D83G/F149S exhibit melting temperature values of 82°C
additional information
concentration-dependent kinetic stability of single mutants I36E and M47D during heat inactivation at 80°C
additional information
-
concentration-dependent kinetic stability of single mutants I36E and M47D during heat inactivation at 80°C
additional information
dimerisation stabilizes against thermal denaturation
additional information
-
dimerisation stabilizes against thermal denaturation
