2.4.2.2: pyrimidine-nucleoside phosphorylase

This is an abbreviated version!
For detailed information about pyrimidine-nucleoside phosphorylase, go to the full flat file.

Word Map on EC 2.4.2.2

2.4.2.2

purine

deaminase

inosine

hypoxanthine

xanthine

phosphorolysis

phosphorylases

synthesis

2'-deoxyribonucleosides

2.4.2.1

5-fluoro-2'-deoxyuridine

ribose-1-phosphate

medicine

Reaction

Synonyms

AmPyNP, BbPyNP, BfPyNP, BsPyNP, deoA, EC 2.4.2.23, GsPyNP, GtPyNP, LpPyNP, nucleoside phosphorylase, PDP, phosphorylase, pyrimidine nucleoside, Py-NPase, PYNP, pynpase, pyrimidine nucleoside phosphorylase, pyrimidine ribonucleoside phosphorylase, pyrimidine-nucleoside phosphorylase, pyrimidine/purine nucleoside phosphorylase, TTHA1771, TtPyNP, udp, UPase

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.2 Pentosyltransferases

2.4.2.2 pyrimidine-nucleoside phosphorylase

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Results	in table
1	Activating Compound
13423	AA Sequence
8	Application
1	CAS Registry Number
18	Cloned(Commentary)
6	Crystallization (Commentary)
85	Disease/ Diagnostics
1	Expression
9	General Information
3	General Stability
10	IC50 Value
7	Inhibitors
12	kcat/KM [mM/s]
2	Ki Value [mM]
50	KM Value [mM]
7	Molecular Weight [Da]
71	Natural Substrates/ Products (Substrates)
136	Organism
1	Oxidation Stability
5	Pathway
10	PDB ID
19	pH Optimum
3	pH Range
2	pH Stability
6	Protein Variants
11	Purification (Commentary)
4	Reaction
5	Reaction Type
45	Reference
1	Renatured (Commentary)
2	Source Tissue
15	Specific Activity [micromol/min/mg]
2	Storage Stability
194	Substrates and Products (Substrate)
11	Subunits
62	Synonyms
1	Systematic Name
15	Temperature Optimum [°C]
5	Temperature Range [°C]
19	Temperature Stability [°C]
20	Turnover Number [1/s]

Purification

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Purification on EC 2.4.2.2 - pyrimidine-nucleoside phosphorylase

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2 entries

harvested with 5 mM 2-mercaptoethanol and 500 mM NaCl in Tris-HCl pH 8, extract desalted with HiPrep 2610, recombinant protein eluted with 0-300 mM NaCl from Super Q Toyopearl 650M column, eluted from Bio-Scale CHT-20-1 column with 10-100 mM NaCl in phosphate-NaOH pH 7, concentrated by ultracentrifugation with 10 kDa cut-off, eluted from HiLoad 16/60 Superdex 200 prep-grade column with 200 mM NaCl in Tris-HCl pH 8, concentrated by ultracentrifugation to 21.7 mg/ml, stored at -70 °C

Thermus thermophilus

Q5SHF9

677372

recombinant enzyme from Escherichia coli strain BL21(DE3) by dialysis, anion exchange chromatography, and gel filtration

recombinant enzymes

recombinant N-terminally His6-tagged Py-NPase from Escherichia coli strain BL21 by nickel affinity chromatography

Parageobacillus thermoglucosidasius

A0A178U4P5

760077

recombinnat His-tagged enzyme by nickel affinity chromatography

Bacillus subtilis

P39142

758599