2.4.2.29: tRNA-guanosine34 preQ1 transglycosylase
This is an abbreviated version!
For detailed information about tRNA-guanosine34 preQ1 transglycosylase, go to the full flat file.
Word Map on EC 2.4.2.29
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2.4.2.29
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anticodon
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queuosine
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wobble
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mobilis
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trnatyr
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shigella
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zymomonas
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hypermodified
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archaeosine
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shigellosis
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preq0
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trna-modifying
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trnaasn
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trnahis
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7-cyano-7-deazaguanine
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minihelix
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7-deazaguanosine
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garcia
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medicine
- 2.4.2.29
-
anticodon
-
queuosine
-
wobble
- mobilis
- trnatyr
- shigella
-
zymomonas
-
hypermodified
-
archaeosine
- shigellosis
- preq0
-
trna-modifying
- trnaasn
- trnahis
- 7-cyano-7-deazaguanine
- minihelix
- 7-deazaguanosine
- garcia
- medicine
Reaction
Synonyms
ArcTGT, guanine insertion enzyme, guanine, queuine-tRNA transglycosylase, Q-insertase, QTRT1, QueTGT, queuine insertase, queuine tRNA ribosyltransferase, queuine tRNA-ribosyltransferase, queuine tRNA-ribosyltransferase subunit 1, ribosyltransferase, queuine transfer ribonucleate, TGT, transfer ribonucleate glycosyltransferase, tRNA guanine transglycosidase, tRNA guanine transglycosylase, tRNA transglycosylase, tRNA-guanine 34 transglycosylase, tRNA-guanine transglycosylase, tRNA–guanine transglycosylase, virulence-associated protein VACC
ECTree
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KM Value
KM Value on EC 2.4.2.29 - tRNA-guanosine34 preQ1 transglycosylase
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0.0012
guanine
wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2
0.0033
guanine
mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2
0.0005
mutant enzyme E235Q, Km less than 0.0005 mM, in 200 mM HEPES, pH 7.3, 20 mM MgCl2
0.0009
pre-queuine 0
wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2
0.0007
wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2
0.032
pre-queuine 1
mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2
0.0009
wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2
0.001
tRNA guanine
mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2
additional information
additional information
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kinetic parameters of recombinant and native enzyme
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