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2.4.2.29: tRNA-guanosine34 preQ1 transglycosylase

This is an abbreviated version!
For detailed information about tRNA-guanosine34 preQ1 transglycosylase, go to the full flat file.

Word Map on EC 2.4.2.29

Reaction

guanine34 in tRNA
+
7-aminomethyl-7-carbaguanine
=
7-aminomethyl-7-carbaguanine34 in tRNA
+
guanine

Synonyms

ArcTGT, guanine insertion enzyme, guanine, queuine-tRNA transglycosylase, Q-insertase, QTRT1, QueTGT, queuine insertase, queuine tRNA ribosyltransferase, queuine tRNA-ribosyltransferase, queuine tRNA-ribosyltransferase subunit 1, ribosyltransferase, queuine transfer ribonucleate, TGT, transfer ribonucleate glycosyltransferase, tRNA guanine transglycosidase, tRNA guanine transglycosylase, tRNA transglycosylase, tRNA-guanine 34 transglycosylase, tRNA-guanine transglycosylase, tRNA–guanine transglycosylase, virulence-associated protein VACC

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.29 tRNA-guanosine34 preQ1 transglycosylase

Purification

Purification on EC 2.4.2.29 - tRNA-guanosine34 preQ1 transglycosylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-tagged TGT purified by Ni2+ affinity chromatography
-
His-Talon column chromatography and Superdex S200 gel filtration
mutant enzyme S90F, S90C and S90A
-
wild-type and mutant enzymes D89E, D89D, D89C and D89A
-