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2.4.2.3: uridine phosphorylase

This is an abbreviated version!
For detailed information about uridine phosphorylase, go to the full flat file.

Word Map on EC 2.4.2.3

Reaction

uridine
+
phosphate
=
Uracil
+
alpha-D-ribose 1-phosphate

Synonyms

apUP, EC 2.4.2.23, L-UrdPase, More, PcUP1, PcUP2, phosphorylase, uridine, pynpase, pyrimidine nucleoside phosphorylase, pyrimidine phosphorylase, pyrimidine/purine nucleoside phosphorylase, StUPh, udp, UDRPase , UP type 1, UP1, UP2, uPA, UPase, UPase-2, UPb, UPH, UPP1, UPP2, UrdPase, uridine phosphorylase, uridine phosphorylase 1, uridine phosphorylase-1, uridine phosphorylase-2, uridine:orthophosphate alpha-D-ribosyltransferase, VchUPh

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.3 uridine phosphorylase

Purification

Purification on EC 2.4.2.3 - uridine phosphorylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, butyl Sepharose column chromatography, and Q Sepharose column chromatography
butyl Sepharose column chromatography and Q-Sepharose column chromatography
Co-NTA agarose column chromatography
homogeneity
native enzyme by hydrophobic interaction and anion exchange chromatography, to homogeneity
-
near homogeneity
-
partial
phosphorylase type I
-
recombinant enzyme from Escherichia coli by ion-exchange chromatography on butyl sepharose resin in the first stage and a Q-sepharose resin in the second, to homogeneity and 96% purity
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by hydrophobic interaction and anion exchange chromatography to 96% homogeneity
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21trxB(DE3) by nickel affinity chromatography and gel filtration
recombinant N-terminally His6-tagged UPP1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant UP1 3.8fold from Escherichia coli strain Rosetta (DE3)