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2.4.2.4: thymidine phosphorylase

This is an abbreviated version!
For detailed information about thymidine phosphorylase, go to the full flat file.

Word Map on EC 2.4.2.4

Reaction

thymidine
+
phosphate
=
thymine
 +
2-deoxy-alpha-D-ribose 1-phosphate

Synonyms

angiogenic factor platelet-derived endothelial cell growth factor/thymidine phosphorylase, animal growth regulators, blood platelet-derived endothelial cell growth factors, blood platelet-derived endothelial cell growth factor, deoA, deoxythymidine phosphorylase, dthdpase, EC 2.4.2.23, gliostatin, gliostatins, GLS, HTP, More, PD-ECGF, PD-ECGF/TP, PD-ECGF/TP platelet-derived endothelial cell growth factor, PDECGF, phosphorylase, thymidine, platelet derived endothelial cell growth factor, platelet derived endothelial cell grwoth factor, platelet derived-endothelial cell growth factor, platelet-derived endothelial cell growth factor, platelet-derived endothelial cell growth factor/thymidine phosphorylase, platelet-derived endotherial cell growth factor, ppnP, pynpase, pyrimidine deoxynucleoside phosphorylase, pyrimidine nucleoside phosphorylase, pyrimidine phosphorylase, pyrimidine/purine nucleoside phosphorylase, TDRPASE, ThdPase, thymidine phosphorylase, thymidine Pi deoxyribosyltransferase, thymidine-orthophosphate deoxyribosyltransferase, thymidine:orthophosphate deoxy-D-ribosyltransferase, Tpase, TYMP

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.4 thymidine phosphorylase

Purification

Purification on EC 2.4.2.4 - thymidine phosphorylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
98% purity
-
ammonium sulfate fractionation
-
chromatography
-
homogeneity
native enzyme
native enzyme 5130fold from liver cytosol to apparent homogeneity by anion exchange and hydrophobic interaction chromatography, and dialysis, followed by hydroxyapatite chromatography
Ni Sepharose 6 column chromatography and Superdex 200 gel filtration
Ni+-chelation-affinity chromatography
-
partial
Q-Sepharose column chromatography and phenyl Sepharose column chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3) by calmodulin affinity chromatography, cleavage of the calmodulin binding protein tag by enterokinase, removal by chromatography on soybean trypsin inhibitor-sepharose
-
recombinant wild-type and mutan enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
separation of uridine phosphorylase and thymidine phosphorylase activities
-