2.4.2.43: lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase
This is an abbreviated version!
For detailed information about lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase, go to the full flat file.
Word Map on EC 2.4.2.43
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2.4.2.43
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photosystem
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synechocystis
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cyanobacterium
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polymyxin
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time-resolved
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photoautotrophically
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elongatus
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epr
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aeruginosa
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thermosynechococcus
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typhimurium
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polymyxin-resistance
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light-regulated
- 2.4.2.43
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photosystem
-
synechocystis
-
cyanobacterium
-
polymyxin
-
time-resolved
-
photoautotrophically
-
elongatus
- epr
- aeruginosa
-
thermosynechococcus
- typhimurium
-
polymyxin-resistance
-
light-regulated
Reaction
Synonyms
4-amino-4-deoxy-L-arabinose transferase, aminoarabinose transferase, arnT, L-Ara4N transferase, undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase
ECTree
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Cloned
Cloned on EC 2.4.2.43 - lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase
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expressed as FLAG-His10-tagged protein in Escherichia coli DH5alpha cells
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overxpression of the L-Ara4N lipid A transferase (ArnT) behind a T7lac promoter
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the 6*His-tagged ArnT gene-encoded plasmids are transformed into Escherichia coli NovaBlue (Novagen) cells for protein expression. The gene is knocked out of the chromosomal DNA of BL21 (BL21(DE3)DELTAArnT), leaving the plasmid-encoded ArnT to be the only ArnT expressed in the system