2.4.2.48: tRNA-guanine15 transglycosylase
This is an abbreviated version!
For detailed information about tRNA-guanine15 transglycosylase, go to the full flat file.
Word Map on EC 2.4.2.48
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2.4.2.48
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horikoshii
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arctgt
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preq0
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pyrococcus
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7-cyano-7-deazaguanine
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anticodon
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d-loop
- 2.4.2.48
- horikoshii
- arctgt
- preq0
-
pyrococcus
- 7-cyano-7-deazaguanine
-
anticodon
-
d-loop
Reaction
Synonyms
archaeosine tRNA-guanine transglycosylase, archaeosine tRNAguanine transglycosylase, ArcTGT, multisite-specific archaeosine tRNA-guanine transglycosylase, Ta1493, TGT, transfer ribonucleic acid guanine15 transglycosylase, tRNA guanine15 transglycosidase, tRNA-guanine transglycosylase
ECTree
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Purification
Purification on EC 2.4.2.48 - tRNA-guanine15 transglycosylase
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ammonium sulfate precipitation, phenyl Sepharose column chromatography, HiTrap Q column chromatography, HiTrap heparin column chromatography, Mono Q column chromatography, and Sephacryl S-200 gel filtration
HiTrap Q-Sepharose and HiTrap Heparin-Sepharose column chromatography
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recombinant TGT 16.8fold from Escherichia coli strain BL21(DE3)/pYB-I-120 by ammonium sulfate fractionation, anion and cation exchange chromatography, and heat treatment