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medicine
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the low substrate specificity of the enzyme is used advantageously for synthesis of nucleoside analogs, some of them of medical interest
synthesis
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development of a practical method for enzymatic synthesis of deoxyguanosine by the combination of transglycosylation with NdRT-II from thymidine to a 2-amino-6-substituted purine, and the hydrolysis reaction with bacterial adenosine deaminase
synthesis
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useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
synthesis
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useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
synthesis
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useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
synthesis
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useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
synthesis
covalent attachment of recombinant Lactobacillus reuteri 2'-deoxyribosyltransferase to Sepabeads EC-EP303. The immobilized enzyme retains 50% of its maximal activity after 17.9 h at 60°C, and 96% activity is observed after storage at 40°C for 110 h. Immobilized enzyme can be recycled for 26 consecutive batch reactions in the synthesis of 2,6-diaminopurine-2'-deoxyriboside with negligible loss of catalytic activity and can be employed in the enzymatic synthesis of different natural and therapeutic nucleosides such as 5-ethyl-2'-deoxyuridine and 5-trifluorothymidine
synthesis
immobilization of 2'-deoxyribosyltransferase from Lactobacillus reuteri on Sepabeads. Immobilized enzyme activity is enhanced 1.21.4fold at 20% of methanol, ethanol, 2-propanol, diethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increased activity is also obtained in presence of 20% acetonitrile. Immobilized enzyme is successfully used in the synthesis of 2'-deoxyxanthosine and 2'-deoxyguanosine using 2'-deoxyuridine as sugar donor and the corresponding poor water-soluble base in the presence of 30% of methanol, ethanol, 2-propanol, ethylene glycol, acetonitrile, and DMSO, giving high nucleoside yields at 4 h
synthesis
compared with NPTs (EC 2.4.2.5), NDTs present the advantage of catalyzing transglycosylation reactions between purine or pyrimidine bases and nucleosides in one enzyme one-pot mode
synthesis
potential of LmPDTas an industrial biocatalyst for enzymatic production of several natural and non-natural therapeutic nucleosides, such as vidarabine (ara A), didanosine (ddI), ddG, or 2'-fluoro-2'-deoxyguanosine
synthesis
TbPDT is proficient in the biosynthesis of numerous therapeutic nucleosides, including didanosine, vidarabine, cladribine, fludarabine, and nelarabine. TbPDT has good potential as an industrial biocatalyst for the synthesis of a wide range of therapeutic nucleosides through an efficient and environmentally friendly methodology
synthesis
the enzyme can be used for in-flow synthesis of nucleoside analogues (2'-deoxy, 2',3'-dideoxy and arabinonucleoside derivatives) of pharmaceutical interest in mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs), method, overview
synthesis
the enzyme is successfully employed in the enzymatic production of several therapeutic nucleosides such as didanosine, vidarabine, and cytarabine
synthesis
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the enzyme is used for a green bioprocess by employing an environmentally friendly methodology to produce floxuridine (5-fluoro-2'-deoxyuridine), a compound with proven anti-tumor activity. The enzyme as enzymatic biocatalyst meets the requirements of high activity, stability, and short reaction times needed for low-cost production in a future preparative application
synthesis
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the enzyme can be used for in-flow synthesis of nucleoside analogues (2'-deoxy, 2',3'-dideoxy and arabinonucleoside derivatives) of pharmaceutical interest in mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs), method, overview
-
synthesis
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useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
-
synthesis
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the enzyme is successfully employed in the enzymatic production of several therapeutic nucleosides such as didanosine, vidarabine, and cytarabine
-
synthesis
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potential of LmPDTas an industrial biocatalyst for enzymatic production of several natural and non-natural therapeutic nucleosides, such as vidarabine (ara A), didanosine (ddI), ddG, or 2'-fluoro-2'-deoxyguanosine
-
synthesis
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the enzyme can be used for in-flow synthesis of nucleoside analogues (2'-deoxy, 2',3'-dideoxy and arabinonucleoside derivatives) of pharmaceutical interest in mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs), method, overview
-
synthesis
-
potential of LmPDTas an industrial biocatalyst for enzymatic production of several natural and non-natural therapeutic nucleosides, such as vidarabine (ara A), didanosine (ddI), ddG, or 2'-fluoro-2'-deoxyguanosine
-
synthesis
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the enzyme is used for a green bioprocess by employing an environmentally friendly methodology to produce floxuridine (5-fluoro-2'-deoxyuridine), a compound with proven anti-tumor activity. The enzyme as enzymatic biocatalyst meets the requirements of high activity, stability, and short reaction times needed for low-cost production in a future preparative application
-
synthesis
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useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
-
synthesis
-
compared with NPTs (EC 2.4.2.5), NDTs present the advantage of catalyzing transglycosylation reactions between purine or pyrimidine bases and nucleosides in one enzyme one-pot mode
-
synthesis
-
potential of LmPDTas an industrial biocatalyst for enzymatic production of several natural and non-natural therapeutic nucleosides, such as vidarabine (ara A), didanosine (ddI), ddG, or 2'-fluoro-2'-deoxyguanosine
-
synthesis
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potential of LmPDTas an industrial biocatalyst for enzymatic production of several natural and non-natural therapeutic nucleosides, such as vidarabine (ara A), didanosine (ddI), ddG, or 2'-fluoro-2'-deoxyguanosine
-
synthesis
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useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
-
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
-
synthesis
-
TbPDT is proficient in the biosynthesis of numerous therapeutic nucleosides, including didanosine, vidarabine, cladribine, fludarabine, and nelarabine. TbPDT has good potential as an industrial biocatalyst for the synthesis of a wide range of therapeutic nucleosides through an efficient and environmentally friendly methodology
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