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0.38 - 0.92
2',3'-dideoxycytidine
0.25 - 22
2'-deoxycytidine
0.2 - 6.8
2'-deoxyinosine
1.14
2'-deoxyuridine
pH 6.5, 40°C
0.04
adenosine
-
reaction with deoxyinosine
0.25
dAMP
-
pH 7.5, 37°C, recombinant wild-type enzyme
4
dCMP
-
pH 7.5, 37°C, recombinant wild-type enzyme
0.092 - 0.45
deoxyadenosine
0.09 - 0.55
deoxycytidine
0.16 - 0.37
deoxyguanosine
0.45
dIMP
-
pH 7.5, 37°C, recombinant wild-type enzyme
50
dTMP
-
above, pH 7.5, 37°C, recombinant wild-type enzyme
15
dUMP
-
pH 7.5, 37°C, recombinant wild-type enzyme
0.13
thymine
-
reaction with deoxyinosine
additional information
additional information
-
0.38
2',3'-dideoxycytidine
wild-type
0.8
2',3'-dideoxycytidine
mutant A15T
0.92
2',3'-dideoxycytidine
mutant G9S
0.25
2'-deoxycytidine
wild-type
0.3
2'-deoxycytidine
wild-type
0.3
2'-deoxycytidine
mutant G9S
0.39
2'-deoxycytidine
mutant A15T
11
2'-deoxycytidine
mutant F13R/D92S/N123S, pH 6.0, 37°C
22
2'-deoxycytidine
mutant F13R/D92S, pH 6.0, 37°C
0.2
2'-deoxyinosine
pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with adenine
0.7
2'-deoxyinosine
pH 6.0, 50°C, recombinant wild-type enzyme, nucleobase release
1.2
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with adenine
2
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with adenine
5.1
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11A, nucleobase release
6.8
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11S, nucleobase release
0.0073
adenine
-
reaction with deoxyinosine
0.008
adenine
-
reaction with deoxyguanosine
0.019
adenine
-
reaction with deoxycytidine
0.021
adenine
-
reaction with deoxyadenosine
0.3
adenine
-
reaction with deoxycytidine
0.48
adenine
-
reaction with thymidine
1.8
adenine
pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with 2'-deoxyinosine
2.9
adenine
pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with 2'-deoxyinosine
2.9
adenine
pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with 2'-deoxyinosine
0.05
cytosine
-
reaction with thymidine
0.073
cytosine
-
reaction with deoxyinosine
0.077
cytosine
-
reaction with deoxyguanosine
0.12
cytosine
-
reaction with deoxyadenosine
0.17
cytosine
-
reaction with deoxycytidine
0.18
cytosine
-
reaction with deoxyinosine
0.19
cytosine
-
reaction with deoxyguanosine
0.22
cytosine
-
reaction with deoxyadenosine
10.79
cytosine
pH 6.5, 40°C
0.092
deoxyadenosine
-
reaction with adenine
0.1
deoxyadenosine
-
reaction with cytosine
0.12
deoxyadenosine
-
reaction with cytosine
0.09
deoxycytidine
-
-
0.09
deoxycytidine
-
reaction with adenine
0.095
deoxycytidine
-
reaction with cytosine
0.52
deoxycytidine
-
reaction with guanine
0.55
deoxycytidine
-
reaction with adenine
0.16
deoxyguanosine
-
reaction with cytosine
0.346
deoxyguanosine
-
reaction with adenine
0.37
deoxyguanosine
-
reaction with cytosine
0.041
deoxyinosine
-
-
0.27
deoxyinosine
-
reaction with guanine
0.3
deoxyinosine
-
reaction with thymine
0.33
deoxyinosine
-
reaction with adenosine
0.35
deoxyinosine
-
reaction with cytosine
3.4
deoxyinosine
-
reaction with adenine
3.5
deoxyinosine
-
reaction with cytosine
0.048
dGMP
-
pH 7.5, 37°C, recombinant wild-type enzyme
5
dGMP
-
pH 7.5, 37°C, recombinant mutant E105A
0.03
guanine
-
reactioin with deoxyinosine
0.48
guanine
-
reaction with deoxycytidine
0.52
guanine
-
reaction with thymidine
0.05
thymidine
-
reaction with cytosine
0.51
thymidine
-
reaction with adenine
0.63
thymidine
-
reaction with guanine
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
kinetics and substrate specifiicty
-