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2.4.2.7: adenine phosphoribosyltransferase

This is an abbreviated version!
For detailed information about adenine phosphoribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.7

Reaction

AMP
+
diphosphate
=
adenine
 +
5-phospho-alpha-D-ribose 1-diphosphate

Synonyms

adenine phosphoribosyl transferase, adenine phosphoribosylpyrophosphate transferase, adenine phosphoribosyltransferase, adenine phosphoribosyltransferase 2, adenione PRTase, adenosine phosphoribosyltransferase, adenylate pyrophosphorylase, adenylic pyrophosphorylase, AMP pyrophosphorylase, AMP-pyrophosphate phosphoribosyltransferase, AMP:pyrophosphate phospho-D-ribosyltransferase, APRT, APRT1, APRT2, APRTase, APRTh, apT, APT1, APT2, APT4, APT5, ApTR, FtAPRT, gpT-1, hAPRT, More, phosphoribosyltransferase, adenine, ScAPRT, SmAPRT1, Smp_054360, SSO2342, transphosphoribosidase, TtAPRT2, TTC1249, TTC1250, TTH1250, TTHA1614, TthAPRT, TthHB27APRT, TT_C1249, TT_C1250, type I PRT

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.7 adenine phosphoribosyltransferase

Cloned

Cloned on EC 2.4.2.7 - adenine phosphoribosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and DNA sequence determination, expression in Escherichia coli BL21 cells as C-terminally His-tagged protein
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in MN-9D cells
-
expression analysis after wounding
expression in Escherichia coli
expression of wild-type and mutants as His-tagged proteins in Escherichia coli B25 cells
-
expression of wild-type enzyme in Escherichia coli strain BL21(DE3) and of the selenomethionine-labeled enzyme in Escherichia coli strain B834
-
gene aprt2, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene apt, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene APT2, DNA and amino acid sequence determination and analysis, expression pattern analysis, recombinant expression in Arabidopsis thaliana plants using the Agrobacterium tumefaciens transfection system
gene Smp_054360, phylogenetic analysis, APRT exists in multiple copies originating from gene duplications at the base of the Schistosoma genus, recombinant expression of His-tagged isozyme SmAPRT1 in Escherichia coli strain BL21(DE3)
gene tth1250, recombinant enzyme expression in Escherichia coli strain BL21(DE3)
gene ttha1614 or aprt2, recombinant overexpression of His6-tagged TtAPRT in Escherichia coli strain BL21(DE3) or recombinant expression of nontagged enzyme in Escherichia coli strain BL21 Codon Plus (DE3)-RIL
gene TT_C1249, sequence comparisons, recombinant coexpression of N-terminally His-tagged ARTh with GDH in Escherichia coli
gene TT_C1250, sequence comparisons
genotyping, genetic screening for mutant genes APRT*Q0 and APRT*J in two families
-
overexpression of the 3 isoforms in Escherichia coli Bl21(DE3)pLysS as His-tagged proteins, the majority of the recombiannt enzyme is in the soluble fraction
recombinant expression of APRT in Escherichia coli strain BL21(DE3)/pER
recombinant expression of N-terminally His6-tagged enzyme
recombinant expression of wild-type and mutant hAPRTs in an APRT-free Saccharomyces cerevisiae strain, which is defective for de novo purine synthesis (strain DS1-2b) an extremely low intracellular concentration of ATP in yeast expressing both variants (Glu104Leu or Tyr105Phe) is observed
transcriptome sequencing and analysis, recombinant overexpression of GFP-tagged enzyme in Trypanosoma cruzi parasites, subcloning in Escherichia coli strain DH5alpha, quantitative RT-PCR expression analysis
-