2.4.2.8: hypoxanthine phosphoribosyltransferase
This is an abbreviated version!
For detailed information about hypoxanthine phosphoribosyltransferase, go to the full flat file.
Word Map on EC 2.4.2.8
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2.4.2.8
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hamster
-
lymphocyte
-
genotoxic
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lesch-nyhan
-
6-thioguanine
-
salvage
-
x-linked
-
ovary
-
gapdh
-
adduct
-
erythrocyte
-
normfinder
-
nucleoside
-
genorm
-
thymidine
-
housekeeping
-
t-lymphocytes
-
uric
-
x-chromosome
-
lymphoblast
-
lymphoblastoid
-
tbp
-
micronuclei
-
bestkeeper
-
transversions
-
chromatid
-
hyperuricemia
-
gout
-
benzoapyrene
-
cytogenetic
-
phosphoribosylpyrophosphate
-
ywhaz
-
comet
-
allopurinol
-
clastogenic
-
n-ethyl-n-nitrosourea
-
aminopterin
-
mutable
-
non-transcribed
-
1,3-butadiene
-
lns
-
repair-deficient
-
sister-chromatid
-
32p-postlabeling
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x-ray-induced
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repair-proficient
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analysis
-
pharmacology
-
o6-methylguanine
-
micronucleated
-
synthesis
-
drug development
-
diagnostics
-
biotechnology
-
molecular biology
-
butadiene
-
x-inactivation
-
medicine
- 2.4.2.8
- hamster
- lymphocyte
-
genotoxic
- lesch-nyhan
- 6-thioguanine
-
salvage
-
x-linked
- ovary
- gapdh
- adduct
- erythrocyte
-
normfinder
- nucleoside
-
genorm
- thymidine
-
housekeeping
- t-lymphocytes
-
uric
-
x-chromosome
- lymphoblast
-
lymphoblastoid
- tbp
-
micronuclei
-
bestkeeper
-
transversions
-
chromatid
- hyperuricemia
- gout
-
benzoapyrene
-
cytogenetic
-
phosphoribosylpyrophosphate
-
ywhaz
- comet
- allopurinol
-
clastogenic
-
n-ethyl-n-nitrosourea
- aminopterin
-
mutable
-
non-transcribed
- 1,3-butadiene
- lns
-
repair-deficient
-
sister-chromatid
-
32p-postlabeling
-
x-ray-induced
-
repair-proficient
- analysis
- pharmacology
- o6-methylguanine
-
micronucleated
- synthesis
- drug development
- diagnostics
- biotechnology
- molecular biology
- butadiene
-
x-inactivation
- medicine
Reaction
Synonyms
6-hydroxypurine phosphoribosyltransferase, 6-mercaptopurine phosphoribosyltransferase, 6-oxo PRTase, 6-oxopurine phosphoribosyltransferase, 6-oxopurine PRTase, Cthe_2254, EcHPRT, GMP pyrophosphorylase, GPRT, gpT-2, guanine phosphoribosyltransferase, guanine PRTase, guanine-hypoxanthine phosphoribosyltransferase, guanosine 5'-phosphate pyrophosphorylase, guanosine phosphoribosyltransferase, guanylate pyrophosphorylase, guanylic pyrophosphorylase, HGPRT, HGPRT-1, HGPRTase, HGXPRT, HGXPRTase, HG[X]PRT, HPRT, HPRT1, HPRTJerusalem, HPT, HXGPRT, hypoxanthine guanine phosphoribosyltransferase, hypoxanthine guanine xanthine phosphoribosyltransferase, hypoxanthine phosphoribosyl transferase, hypoxanthine phosphoribosyltransferase, hypoxanthine phosphoribosyltransferase 1, hypoxanthine PRTase, hypoxanthine-guanine phosphoribosyl transferase, hypoxanthine-guanine phosphoribosyltransferase, hypoxanthine-guanine phosphoribosyltransferase/adenylate kinase, hypoxanthine-guanine-(xanthine) phosphoribosyltransferase, hypoxanthine-guanine-xanthine phosphoribosyltransferase, hypoxanthine-guanine-[xanthine]-phosphoribosyltransferase, hypoxanthine-xanthine-guanine phosphoribosyltransferase, IMP pyrophosphorylase, IMP-GMP pyrophosphorylase, inosinate pyrophosphorylase, inosine 5'-phosphate pyrophosphorylase, inosine monophosphate:pyrophosphate phosphoribosyltransferase, inosinic acid pyrophosphorylase, inosinic pyrophosphorylase, More, MSMEG_6110, MtHGPRT, Pf HGXPRT, PF3D7_1012400, PfHGXPRT, PhHGXPRT, phosphoribosyltransferase, 6-mercaptopurine, phosphoribosyltransferase, hypoxanthine, PhPRT, plasma hypoxanthine-guanine phosphoribosyl transferase, PRT, purine-6-thiol phosphoribosyltransferase, PvHGPRT, SmHGPRT1, SSO2424, Tb927.10.1400, TcHPRT, transphosphoribosidase, TthHPRT, TT_C1766, ZgHGPRT/AMPK
ECTree
2.4.2.8 hypoxanthine phosphoribosyltransferaseAdvanced search results
results (
results (KM Value
KM Value on EC 2.4.2.8 - hypoxanthine phosphoribosyltransferase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.001
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 22°C, recombinant mutant F36L, with xanthine
0.0044
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 22°C, recombinant mutant F36K, with hypoxanthine
0.0065
5-phospho-alpha-D-ribose 1-diphosphate
with guanine as cosubstrate, at pH 7.5 and 25°C
0.011
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 22°C, recombinant mutant F36G, with hypoxanthine
0.0141
5-phospho-alpha-D-ribose 1-diphosphate
with hypoxanthine as cosubstrate, at pH 7.5 and 25°C
0.025
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant IMP-activated wild-type enzyme
0.027
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 22°C, recombinant mutant F36L, with hypoxanthine
0.029
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 22°C, recombinant mutant F36K, with guanine
0.03
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 22°C, recombinant wild-type enzyme, with hypoxanthine
0.031
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.5, 37°C, recombinant wild-type enzyme, with hypoxanthine
0.032
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.5, 37°C, recombinant wild-type enzyme
0.032
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.5, 37°C, recombinant wild-type enzyme, with guanine
0.035
5-phospho-alpha-D-ribose 1-diphosphate
-
recombinant enzyme, with hypoxanthine
0.035
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 22°C, recombinant wild-type enzyme, with hypoxanthine
0.041
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 22°C, recombinant mutant F36L, with guanine
0.05
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 22°C, recombinant mutant F36G, with guanine
0.053
5-phospho-alpha-D-ribose 1-diphosphate
IMP-activated enzyme, at pH 7.4 and 23°C
0.06
5-phospho-alpha-D-ribose 1-diphosphate
with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C
0.076
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 22°C, recombinant wild-type enzyme, with guanine
0.079
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 22°C, recombinant wild-type enzyme, with xanthine
0.083
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.5, 37°C, recombinant mutant G69S
0.094
5-phospho-alpha-D-ribose 1-diphosphate
activated enzyme, with xanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.094
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant IMP-activated mutant W181Y
0.102
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 22°C, recombinant mutant L44F, with hypoxanthine
0.103
5-phospho-alpha-D-ribose 1-diphosphate
recombinant wild-type enzyme, pH 7.4, 25°C
0.104
5-phospho-alpha-D-ribose 1-diphosphate
with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C
0.112
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 50°C, with guanine
0.116
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 22°C, recombinant mutant L44F, with guanine
0.116
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, with guanine
0.119
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, with hypoxanthine
0.123
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 25°C, with hypoxanthine
0.125
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 25°C, with guanine
0.132
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.5, 37°C, recombinant mutant L67M
0.133
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 12 mM MgCl2
0.138
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 50°C, with hypoxanthine
0.151
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 22°C, recombinant mutant L44F, with xanthine
0.183
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.201
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 22°C, recombinant wild-type enzyme, with guanine
0.22
5-phospho-alpha-D-ribose 1-diphosphate
recombinant enzyme, pH 8.0, 70°C
0.233
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant IMP-activated mutant W181F
0.251
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant IMP-activated mutant W181S
0.256
5-phospho-alpha-D-ribose 1-diphosphate
activated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.263
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, with guanine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.275
5-phospho-alpha-D-ribose 1-diphosphate
activated enzyme, with guanine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.277
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, with xanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.327
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 12 mM MgCl2, in presence of 0.05 mM IMP
0.36
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 25°C, recombinant enzyme, with guanine
0.546
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 28°C, recombinant chimeric mutant enzyme with substrate hypoxanthine
0.546
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 28°C, recombinant chimeric mutant enzyme with substrate hypoxanthine
0.972
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, with hypoxanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C
1.075
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 28°C, recombinant chimeric mutant enzyme, with substrate guanine
1.075
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 28°C, recombinant chimeric mutant enzyme, with substrate guanine
1.08
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, at pH 7.4 and 23°C
1.084
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, with xanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C
1.084
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant nonactivated wild-type enzyme
1.548
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, with guanine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C
2.039
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 25°C, recombinant enzyme, with hypoxanthine
2.286
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant nonactivated mutant W181S
2.783
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant nonactivated mutant W181Y
3.668
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant nonactivated mutant W181F
0.127
5-phosphoribosyl 1-diphosphate
recombinant enzyme, with guanine
0.138
5-phosphoribosyl 1-diphosphate
recombinant enzyme, with hypoxanthine
0.002
guanine
unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.0025
guanine
activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.0028
guanine
recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate
0.0048
guanine
unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.0105
guanine
with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C
0.025
guanine
-
pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate
0.135
guanine
with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C
0.362
guanine
-
recombinant enzyme, with 5-phosphoribosyl 1-diphosphate
0.001
hypoxanthine
-
pH 7.4, 22°C, recombinant wild-type enzyme and mutant L44F
0.0018
hypoxanthine
pH 7.4, 28°C, recombinant chimeric mutant enzyme
0.0044
hypoxanthine
recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate
0.005
hypoxanthine
unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.005
hypoxanthine
unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.0055
hypoxanthine
recombinant wild-type enzyme, pH 7.4, 25°C
0.0075
hypoxanthine
activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.05
hypoxanthine
-
pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate
0.06
hypoxanthine
-
recombinant enzyme, with 5-phosphoribosyl 1-diphosphate
0.126
hypoxanthine
-
recombinant enzyme, with 5-phosphoribosyl 1-diphosphate, pH 8.5
0.036
xanthine
activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.045
xanthine
unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.078
xanthine
unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.17
xanthine
-
recombinant enzyme, with 5-phosphoribosyl 1-diphosphate, pH 8.5
additional information
additional information
-
Km-values of mutant enzymes
-
additional information
additional information
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Km-values, loop II-deletion mutant
-
additional information
additional information
-
wild-type and mutant T47K
-
additional information
additional information
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Km values of mutant enzymes for hypoxanthine, guanine, diphosphate, 5-phosphoribosyl 1-diphosphate, inosine monophosphate
-
additional information
additional information
-
wild-type and mutants: Km values for substrates hypoxanthine, guanine, xanthine, IMP, GMP, XMP
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
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Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
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Michaelis-Menten kinetics
-
additional information
additional information
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kinetic analysis in the forward and reverse reaction of the wild-type enzyme and diverse mutant enzymes, overview
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additional information
additional information
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steady-state kinetics and dissociation constants
-
additional information
additional information
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kinetic analysis of the branched bi-enzyme system, detailed overview
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additional information
additional information
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steady-state kinetics, recombinant enzyme, overview
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additional information
additional information
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no significant change in KM with temperature (40-70°C) for all substrates tested
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