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2.4.2.8: hypoxanthine phosphoribosyltransferase

This is an abbreviated version!
For detailed information about hypoxanthine phosphoribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.8

Reaction

IMP
+
diphosphate
=
hypoxanthine
 +
5-phospho-alpha-D-ribose 1-diphosphate

Synonyms

6-hydroxypurine phosphoribosyltransferase, 6-mercaptopurine phosphoribosyltransferase, 6-oxo PRTase, 6-oxopurine phosphoribosyltransferase, 6-oxopurine PRTase, Cthe_2254, EcHPRT, GMP pyrophosphorylase, GPRT, gpT-2, guanine phosphoribosyltransferase, guanine PRTase, guanine-hypoxanthine phosphoribosyltransferase, guanosine 5'-phosphate pyrophosphorylase, guanosine phosphoribosyltransferase, guanylate pyrophosphorylase, guanylic pyrophosphorylase, HGPRT, HGPRT-1, HGPRTase, HGXPRT, HGXPRTase, HG[X]PRT, HPRT, HPRT1, HPRTJerusalem, HPT, HXGPRT, hypoxanthine guanine phosphoribosyltransferase, hypoxanthine guanine xanthine phosphoribosyltransferase, hypoxanthine phosphoribosyl transferase, hypoxanthine phosphoribosyltransferase, hypoxanthine phosphoribosyltransferase 1, hypoxanthine PRTase, hypoxanthine-guanine phosphoribosyl transferase, hypoxanthine-guanine phosphoribosyltransferase, hypoxanthine-guanine phosphoribosyltransferase/adenylate kinase, hypoxanthine-guanine-(xanthine) phosphoribosyltransferase, hypoxanthine-guanine-xanthine phosphoribosyltransferase, hypoxanthine-guanine-[xanthine]-phosphoribosyltransferase, hypoxanthine-xanthine-guanine phosphoribosyltransferase, IMP pyrophosphorylase, IMP-GMP pyrophosphorylase, inosinate pyrophosphorylase, inosine 5'-phosphate pyrophosphorylase, inosine monophosphate:pyrophosphate phosphoribosyltransferase, inosinic acid pyrophosphorylase, inosinic pyrophosphorylase, More, MSMEG_6110, MtHGPRT, Pf HGXPRT, PF3D7_1012400, PfHGXPRT, PhHGXPRT, phosphoribosyltransferase, 6-mercaptopurine, phosphoribosyltransferase, hypoxanthine, PhPRT, plasma hypoxanthine-guanine phosphoribosyl transferase, PRT, purine-6-thiol phosphoribosyltransferase, PvHGPRT, SmHGPRT1, SSO2424, Tb927.10.1400, TcHPRT, transphosphoribosidase, TthHPRT, TT_C1766, ZgHGPRT/AMPK

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.8 hypoxanthine phosphoribosyltransferase

Storage Stability

Storage Stability on EC 2.4.2.8 - hypoxanthine phosphoribosyltransferase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, dilute aqueous purified enzyme preparation, a few weeks
-20°C, purified enzyme, 6 mM 5-phospho-alpha-D-ribose 1-diphosphate, 2 mM MgCl2, stable for at least a month
-
-20°C, several months
-
-70°C, wild-type enzyme and mutants, except mutant S95C, stable up to 6 months
-
-80°C, mutant and wild-type enzyme, stable up to 3 years
-
0°C, 10 mM phosphate, pH 7.1, 1 mM DTT, 10 mM Mg2+, 2 h, no loss of activity
-
4°C, 50 mM Tris-HCl, pH 7.8, 1 M KCl, 10 mM MgCl2, stable at least 1 month
-
4°C, 50 mM Tris-HCl, pH 7.8, complete loss of activity after 4 days
-
4°C, freshly purified enzyme, 10 mM phosphate, pH 6.8, 10 mM DTT, 1 mM 5-phosphoribosyl 1-diphosphate, rapid loss of 90% activity within 48 h
-
4°C, purified recombinant enzyme at 0.5 mg/ml, 100 mM Tris-HCl, pH 7.4, 12 mM MgCl2, 3 months, no loss of activity
5°C, purified enzyme, 10 mM phosphate, pH 6.8, 1 mM DTT, 0.2 mM 5-phosphoribosyl 1-diphosphate, 0.06 hypoxanthine, loss of 7% activity after 4-8 weeks
-
frozen, partially purified enzyme, 4 months without loss of activity
-