2.4.2.9: uracil phosphoribosyltransferase

This is an abbreviated version!
For detailed information about uracil phosphoribosyltransferase, go to the full flat file.

Reaction

Synonyms

AFR052C, AFR052Cp, AGOS_AFR052C, AgUPRT, CD-UPRT, cytosine deaminase-uracil phosphoribosyltransferase, cytosine deaminase/uracil phosphoribosyltransferase, More, MtUPRT, phosphoribosyltransferase, uracil, Rv3309c, TtUPRT, TtUPRT3, UK/UPRT1, UMP pyrophosphorylase, UMP:pyrophosphate phosphoribosyltransferase, UPP, UPRT, UPRTase, uracil phosphoribosyl transferase, uridine 5'-phosphate pyrophosphorylase, uridine kinase-like protein (uracil phosphoribosyltransferase), uridine kinase/uracil phosphoribosyltransferase 1, uridine monophosphate pyrophosphorylase, uridylate pyrophosphorylase, uridylic pyrophosphorylase

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.2 Pentosyltransferases

                2.4.2.9 uracil phosphoribosyltransferase

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Results	in table
22	Activating Compound
44618	AA Sequence
15	Application
1	CAS Registry Number
34	Cloned(Commentary)
7	Crystallization (Commentary)
312	Disease/ Diagnostics
24	General Information
10	General Stability
1	IC50 Value
49	Inhibitors
5	Ki Value [mM]
46	KM Value [mM]
4	Localization
43	Metals/Ions
33	Molecular Weight [Da]
32	Natural Substrates/ Products (Substrates)
368	Organism
5	Pathway
104	PDB ID
21	pH Optimum
7	pH Range
2	pI Value
20	Protein Variants
21	Purification (Commentary)
10	Reaction
1	Reaction Type
66	Reference
30	Source Tissue
19	Specific Activity [micromol/min/mg]
12	Storage Stability
109	Substrates and Products (Substrate)
36	Subunits
88	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
2	Temperature Range [°C]
14	Temperature Stability [°C]
8	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.4.2.9 - uracil phosphoribosyltransferase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-15°C, partially purified enzyme, loss of 10% activity within 2 weeks

Lactobacillus bifidus

-

638358

-20°C, 20 mM Hepes-KOH, pH 7.4, 1 mM DTT, stable for 3 days

Giardia intestinalis

-

638368

-70°C, 20 mM Hepes-KOH, pH 7.4, 1-2 months without loss of activity

Giardia intestinalis

-

638368

0°C, enzyme concentration 0.001 mg/ml, 50% loss of activity in 1 h

[Bacillus] caldolyticus

P70881

638370

4°C for 13 days or 1 year at -70°C, ethylene glycol, significantly increases stability with virtually no loss of activity

Escherichia coli

-

638362

4°C, 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 2 mM 2-mercaptoethanol, 44% loss of activity after 8 d

Escherichia coli

-

638362

4°C, concentrated enzyme is stable for several months, on dilution occurs rapid loss of activity

[Bacillus] caldolyticus

P70881

638370

4°C, purified detagged recombinant enzyme, in Tris-NaCl buffer, stable for at least 1 week

Eremothecium gossypii

Q754M0

759853

4°C, purified enzyme, TMD50 buffer, stable for at least 1 week

Toxoplasma gondii

-

638367

4°C, rapid loss of activity when stored in presence of Mg2+

Acholeplasma laidlawii

-

638363

4°C, Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT, 1 mM UMP, loss of 40% activity in 1 week

Candida albicans

-

638366

4°C, Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT, loss of 70% activity in 4 days

Candida albicans

-

638366