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2.4.99.18: dolichyl-diphosphooligosaccharide-protein glycotransferase

This is an abbreviated version!
For detailed information about dolichyl-diphosphooligosaccharide-protein glycotransferase, go to the full flat file.

Word Map on EC 2.4.99.18

Reaction

dolichyl diphosphooligosaccharide
+
[protein]-L-asparagine
=
dolichyl diphosphate
 +
a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine

Synonyms

AglB, AglB-L, AlgB, asparagine N-glycosyltransferase, DGL1, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2, dolichyl-diphosphooligosaccharide-protein glycosyltransferase, dolichyldiphosphooligosaccharide-protein oligosaccharyltransferase, EC 2.4.1.119, glycosyltransferase, dolichyldiphosphooligosaccharide-protein, glycosyltransferase, dolichylpyrophosphodiacetylchitobiose-protein, L.m.STT3, Lm STT3, oligomannosyltransferase, oligosaccharide transferase, oligosaccharyl transferase, oligosaccharyl transferase 16 kDa subunit, oligosaccharyl transferase subunit epsilon, oligosaccharyl transferase subunit OST2, oligosaccharyltransferase, oligosaccharyltransferase complex, oligosaccharyltransferase STT3, oligosaccharyltransferase, dolichyldiphosphoryloligosaccharide-protein, OST, OST-A, OST-B, OST-I, OST-II, OST-III, OST1, OST2, OST3, Ost3p, OST4, OST5, Ost6p, OSTC(I), OSTC(II), OSTC(III), OTase, PglB, PglB oligosaccharyltransferase, PglB1, PglL, PilO, STT3, STT3 protein, STT3-1, STT3-2, STT3-3, STT3-4, Swp1p, TbSTT3A, TbSTT3B, TbSTT3C, Wbp1p, yeast oligosaccharyl transferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.99 Transferring other glycosyl groups
                2.4.99.18 dolichyl-diphosphooligosaccharide-protein glycotransferase

Crystallization

Crystallization on EC 2.4.99.18 - dolichyl-diphosphooligosaccharide-protein glycotransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
C-terminal globular domain of the of the smallest AglB, purified recombinant wild-type and selenomethionine-labeled AgBs, sitting and hanging drop vapor diffusion methods, crystals grow from a hanging drop with a 1:1 volume ratio of the protein stock solution containing 20 mg/mL protein in 20 mM Tris buffer, pH 8.0, and the reservoir solution containing 0.1 M MES buffer, pH 6.5, and 12.5% PEG 3350, at 20°C, soaking of crystals in reservoir solution with 20% ethylene glycol added for cryoprotection, X-ray diffraction structure determination and analysis at 1.75-1.94 A resolution
sitting drop vapor diffusion method, crystal structure of the C-terminal globular domain of the AglB paralog, AglB-Long, at 1.9 A resolutions
2.8 A resolution crystal structure of the C-terminal globular domain, hanging drop method
hanging drop crystallization with 10 mM Tris-HCl, pH 8.0, and reservoir solution (0.1 M sodium cacodylate, pH 6.5, 18% polyethylene glycol 8000, 0.2 M calcium acetate, 20°C, cryoprotection with 0.1 M MES, pH 6.5, polyethylene glycol 8000, and 0.2 M calcium acetate)
2.7 A resolution crystal structure of the C-terminal soluble domain of Pyrococcus STT3
-
sitting-drop vapour diffusion method
12 A resolution cryoelectron microscopy structure
-
sitting drop vapor diffusion method, dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit OST6