2.4.99.18: dolichyl-diphosphooligosaccharide-protein glycotransferase

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For detailed information about dolichyl-diphosphooligosaccharide-protein glycotransferase, go to the full flat file.

Word Map on EC 2.4.99.18

2.4.99.18

n-glycosylation

n-linked

glycans

lipid-linked

sequons

asparagine-linked

jejuni

campylobacter

dolichol

ribophorins

otase

cotranslational

bloc

dolichol-linked

translocon

glc3man9glcnac2

heptasaccharide

s-layer

underglycosylation

preassembled

mannosyltransferases

glycoengineering

hypoglycosylation

hetero-oligomeric

single-subunit

translocon-associated

man9glcnac2

lari

drug development

biotechnology

medicine

n-glycoproteins

glycoproteomics

pilins

Reaction

a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine

Synonyms

AglB, AglB-L, AlgB, asparagine N-glycosyltransferase, DGL1, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2, dolichyl-diphosphooligosaccharide-protein glycosyltransferase, dolichyldiphosphooligosaccharide-protein oligosaccharyltransferase, EC 2.4.1.119, glycosyltransferase, dolichyldiphosphooligosaccharide-protein, glycosyltransferase, dolichylpyrophosphodiacetylchitobiose-protein, L.m.STT3, Lm STT3, oligomannosyltransferase, oligosaccharide transferase, oligosaccharyl transferase, oligosaccharyl transferase 16 kDa subunit, oligosaccharyl transferase subunit epsilon, oligosaccharyl transferase subunit OST2, oligosaccharyltransferase, oligosaccharyltransferase complex, oligosaccharyltransferase STT3, oligosaccharyltransferase, dolichyldiphosphoryloligosaccharide-protein, OST, OST-A, OST-B, OST-I, OST-II, OST-III, OST1, OST2, OST3, Ost3p, OST4, OST5, Ost6p, OSTC(I), OSTC(II), OSTC(III), OTase, PglB, PglB oligosaccharyltransferase, PglB1, PglL, PilO, STT3, STT3 protein, STT3-1, STT3-2, STT3-3, STT3-4, Swp1p, TbSTT3A, TbSTT3B, TbSTT3C, Wbp1p, yeast oligosaccharyl transferase

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.99 Transferring other glycosyl groups

2.4.99.18 dolichyl-diphosphooligosaccharide-protein glycotransferase

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Results	in table
3	Activating Compound
7433	AA Sequence
7	Application
1	CAS Registry Number
33	Cloned(Commentary)
9	Crystallization (Commentary)
57	Disease/ Diagnostics
1	Expression
34	General Information
5	General Stability
29	Inhibitors
38	KM Value [mM]
66	Localization
15	Metals/Ions
38	Molecular Weight [Da]
64	Natural Substrates/ Products (Substrates)
211	Organism
4	Pathway
31	PDB ID
8	pH Optimum
1	pH Range
4	Posttranslational Modification
90	Protein Variants
23	Purification (Commentary)
1	Reaction
1	Reaction Type
178	Reference
41	Source Tissue
4	Specific Activity [micromol/min/mg]
4	Storage Stability
224	Substrates and Products (Substrate)
48	Subunits
284	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
6	Temperature Stability [°C]

General Stability

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General Stability on EC 2.4.99.18 - dolichyl-diphosphooligosaccharide-protein glycotransferase

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DAD1 might provide structural and functional integrity for the OST complex

Saccharomyces cerevisiae

720164

glycerol, 25%, stabilizes

Saccharomyces cerevisiae

488505

phosphatidylcholine stabilizes detergent-solubilized enzyme

Canis lupus familiaris

488501

solubilization with detergents inactivates

Gallus gallus

488500

strongly stabilized by addition of phospholipids upon detergent solubilization, phosphatidylcholine is twice as effective as phosphatidylethanolamine

Saccharomyces cerevisiae

P48439

488513