2.4.99.18: dolichyl-diphosphooligosaccharide-protein glycotransferase

This is an abbreviated version!
For detailed information about dolichyl-diphosphooligosaccharide-protein glycotransferase, go to the full flat file.

Word Map on EC 2.4.99.18

2.4.99.18

n-glycosylation

n-linked

glycans

lipid-linked

sequons

asparagine-linked

jejuni

campylobacter

dolichol

ribophorins

otase

cotranslational

bloc

dolichol-linked

translocon

glc3man9glcnac2

heptasaccharide

s-layer

underglycosylation

preassembled

mannosyltransferases

glycoengineering

hypoglycosylation

hetero-oligomeric

single-subunit

translocon-associated

man9glcnac2

lari

drug development

biotechnology

medicine

n-glycoproteins

glycoproteomics

pilins

Reaction

dolichyl diphosphooligosaccharide

+

[protein]-L-asparagine

=

dolichyl diphosphate

+

a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine

Synonyms

AglB, AglB-L, AlgB, asparagine N-glycosyltransferase, DGL1, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2, dolichyl-diphosphooligosaccharide-protein glycosyltransferase, dolichyldiphosphooligosaccharide-protein oligosaccharyltransferase, EC 2.4.1.119, glycosyltransferase, dolichyldiphosphooligosaccharide-protein, glycosyltransferase, dolichylpyrophosphodiacetylchitobiose-protein, L.m.STT3, Lm STT3, oligomannosyltransferase, oligosaccharide transferase, oligosaccharyl transferase, oligosaccharyl transferase 16 kDa subunit, oligosaccharyl transferase subunit epsilon, oligosaccharyl transferase subunit OST2, oligosaccharyltransferase, oligosaccharyltransferase complex, oligosaccharyltransferase STT3, oligosaccharyltransferase, dolichyldiphosphoryloligosaccharide-protein, OST, OST-A, OST-B, OST-I, OST-II, OST-III, OST1, OST2, OST3, Ost3p, OST4, OST5, Ost6p, OSTC(I), OSTC(II), OSTC(III), OTase, PglB, PglB oligosaccharyltransferase, PglB1, PglL, PilO, STT3, STT3 protein, STT3-1, STT3-2, STT3-3, STT3-4, Swp1p, TbSTT3A, TbSTT3B, TbSTT3C, Wbp1p, yeast oligosaccharyl transferase

ECTree

2.4 Glycosyltransferases

2.4.99 Transferring other glycosyl groups

2.4.99.18 dolichyl-diphosphooligosaccharide-protein glycotransferase

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Results	in table
3	Activating Compound
7433	AA Sequence
7	Application
1	CAS Registry Number
33	Cloned(Commentary)
9	Crystallization (Commentary)
57	Disease/ Diagnostics
1	Expression
34	General Information
5	General Stability
29	Inhibitors
38	KM Value [mM]
66	Localization
15	Metals/Ions
38	Molecular Weight [Da]
64	Natural Substrates/ Products (Substrates)
211	Organism
4	Pathway
31	PDB ID
8	pH Optimum
1	pH Range
4	Posttranslational Modification
90	Protein Variants
23	Purification (Commentary)
1	Reaction
1	Reaction Type
178	Reference
41	Source Tissue
4	Specific Activity [micromol/min/mg]
4	Storage Stability
224	Substrates and Products (Substrate)
48	Subunits
284	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
6	Temperature Stability [°C]

KM Value

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KM Value on EC 2.4.99.18 - dolichyl-diphosphooligosaccharide-protein glycotransferase

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2

Ac-Asn-Ala-Thr-NH2

-

-

1

Ac-Asn-Leu-Thr-NH2

-

-

0.08 - 0.143

acetyl-Asn-Ala-Thr

show

0.278

acetyl-Asn-Lys-Thr

-

-

0.00087

acetyl-DFNAT-(4-nitrophenylalanine)-NH2

Campylobacter jejuni

-

-

0.00122

acetyl-DFNVT-(4-nitrophenylalanine)-NH2

Campylobacter jejuni

-

-

0.0008

acetyl-DQNAT-(4-nitrophenylalanine)-NH2

Campylobacter jejuni

-

-

0.003

acetyl-DVNAS-(4-nitrophenylalanine)-NH2

Campylobacter jejuni

-

-

0.00106

acetyl-DVNAT-(4-nitrophenylalanine)-NH2

Campylobacter jejuni

-

-

0.00144

acetyl-DVNVT-(4-nitrophenylalanine)-NH2

Campylobacter jejuni

-

-

0.0225

acetyl-EVNAT-(4-nitrophenylalanine)-NH2

Campylobacter jejuni

-

-

0.3 - 0.358

Ala-Leu-Gln-Asn-Ala-Thr-Arg

show

0.3

Ala-Leu-Glu-Asn-Ala-Thr-Arg-NH2

-

-

0.56 - 2.09

Asn-Ala-Thr

show

3.3

Asn-Asp-Thr

-

-

0.25

benzoyl-Asn-Leu-Thr-N-methyl-threonine

-

-

0.072 - 0.23

diphenyl-Ala-Leu-Glu-Asn-Ala-Thr-Arg-NH2

show

0.23

diphenyl-AlaLeu-Glu-Asn-Ala-Thr-Arg-NH2

-

-

0.0012

dolichyl diphosphochitobiose

Saccharomyces cerevisiae

-

-

0.0005

dolichyl diphosphochitobiose-(Man)9-(Glc)3

Saccharomyces cerevisiae

-

-

0.0315

dolichyl-diphosphochitobiose-(Man)9

Saccharomyces cerevisiae

-

-

0.021

dolichyl-diphosphochitobiose-(Man)9-(Glc)3

Saccharomyces cerevisiae

-

-

12.5

N-benzoyl-Asn-Gly-D-allo-Thr-NHCH3

-

-

4.3

N-benzoyl-Asn-Gly-L-threo-beta-hydroxynorvaline-NHCH3

-

-

0.6

N-benzoyl-Asn-Gly-Ser-NHCH3

-

-

0.16

N-benzoyl-Asn-Gly-Thr-NHCH3

-

-

0.00015

Nalpha-Ac-Asn-Tyr-Thr-NH2

Saccharomyces cerevisiae

-

-

0.05 - 0.6

Tyr-Asn-Leu-Thr-Ser-Val

show

0.08 - 0.127

Tyr-Gln-Ser-Asn-Ser-Thr-Met

show

additional information

additional information

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-

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Release 2023.1 (January 2023)