2.4.99.19: undecaprenyl-diphosphooligosaccharide-protein glycotransferase
This is an abbreviated version!
For detailed information about undecaprenyl-diphosphooligosaccharide-protein glycotransferase, go to the full flat file.
Word Map on EC 2.4.99.19
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2.4.99.19
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campylobacter
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n-glycosylation
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jejuni
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n-linked
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glycans
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lipid-linked
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lari
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sequons
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asparagine-linked
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meningitidis
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single-subunit
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glycoengineering
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ribophorin
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dolichols
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pilins
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medicine
- 2.4.99.19
- campylobacter
-
n-glycosylation
- jejuni
-
n-linked
- glycans
-
lipid-linked
- lari
-
sequons
-
asparagine-linked
- meningitidis
-
single-subunit
-
glycoengineering
-
ribophorin
- dolichols
- pilins
- medicine
Reaction
Synonyms
bacterial oligosaccharyltransferase, bacterial OST, N-oligosaccharyltransferase, N-OTase, oligosaccharyl transferase, oligosaccharyltransferase, oligosacharyltransferase, OST, OT, OTase, PglB, PglB oligosaccharyltransferase, PglB protein, PglB1, PglB2
ECTree
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Purification
Purification on EC 2.4.99.19 - undecaprenyl-diphosphooligosaccharide-protein glycotransferase
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recombinant His-tagged full-length PglB from Escherichia coli strain BL21(DE3) in the membrane fraction by ultracentrifugation, recombinant GST-tagged wild-type and selenomethionine-labeled C-terminal globular domain of PglB from Escherichia coli strain BL21(DE3)pLysS by glutathione affinity chromatography and cleavage of the tag by 3C protease, followed by to reductive methylation of the lysine residues, gel filtration, and anion exchange chromatography
recombinant His-tagged PglB from Escherichia coli strain C43(DE3) by nickel affinity chromatography
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recombinant His10-tagged wild-type and mutant enzymes from Escherichia coli strain SCM6