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2.4.99.19: undecaprenyl-diphosphooligosaccharide-protein glycotransferase

This is an abbreviated version!
For detailed information about undecaprenyl-diphosphooligosaccharide-protein glycotransferase, go to the full flat file.

Word Map on EC 2.4.99.19

Reaction

tritrans,heptacis-undecaprenyl diphosphooligosaccharide
+
[protein]-L-asparagine
=
tritrans,heptacis-undecaprenyl diphosphate
+
a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine

Synonyms

bacterial oligosaccharyltransferase, bacterial OST, N-oligosaccharyltransferase, N-OTase, oligosaccharyl transferase, oligosaccharyltransferase, oligosacharyltransferase, OST, OT, OTase, PglB, PglB oligosaccharyltransferase, PglB protein, PglB1, PglB2

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.99 Transferring other glycosyl groups
                2.4.99.19 undecaprenyl-diphosphooligosaccharide-protein glycotransferase

Purification

Purification on EC 2.4.99.19 - undecaprenyl-diphosphooligosaccharide-protein glycotransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged full-length PglB from Escherichia coli strain BL21(DE3) in the membrane fraction by ultracentrifugation, recombinant GST-tagged wild-type and selenomethionine-labeled C-terminal globular domain of PglB from Escherichia coli strain BL21(DE3)pLysS by glutathione affinity chromatography and cleavage of the tag by 3C protease, followed by to reductive methylation of the lysine residues, gel filtration, and anion exchange chromatography
recombinant His-tagged PglB from Escherichia coli strain C43(DE3) by nickel affinity chromatography
-
recombinant His10-tagged wild-type and mutant enzymes from Escherichia coli strain SCM6