Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.5.1.106: tryprostatin B synthase

This is an abbreviated version!
For detailed information about tryprostatin B synthase, go to the full flat file.

Word Map on EC 2.5.1.106

Reaction

dimethylallyl diphosphate
+
brevianamide F
=
diphosphate
+
tryprostatin B

Synonyms

brevianamide F prenyltransferase, cyclic dipeptide C2-prenyltransferase FtmPT1, FtmPT1, indole prenyltransferase, tryprostatin B synthase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.106 tryprostatin B synthase

Engineering

Engineering on EC 2.5.1.106 - tryprostatin B synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E102Q
mutation reduces the production of tryprostatin B to undetectable amounts
G115A
G115A/Y205C
-
significantly increased acceptance of 1-naphthol and other hydroxynaphthalenes by the mutant enzyme
G115I
complete loss of activity
G115L
formation of tryprostatin B is abolished, formation of a new product occurs
G115T
H279F
70% of wild-type activity
Y205A
mutant produces 70.3% C3-regularly prenylated product
Y205C
Y205D
mutant produces 55.8% C3-regularly prenylated product and 3.6% C3-reversely prenylated product
Y205E
mutant produces 57.6% C3-regularly prenylated product and 3% C3-reversely prenylated product
Y205F
Y205G
mutant produces 92% C3-regularly prenylated product and 12.2% C3-reversely prenylated product
Y205H
mutant produces 60.7% C3-regularly prenylated product
Y205I
Y205K
mutant produces 110% C3-regularly prenylated product and 1.6% C3-reversely prenylated product
Y205L
Y205M
Y205N
mutant produces 70% C3-regularly prenylated product and 2.8% C3-reversely prenylated product
Y205P
mutant produces 38.7% C3-regularly prenylated product
Y205Q
mutant produces 33.7% C3-regularly prenylated product
Y205R
mutant produces 77% C3-regularly prenylated product and 13.2% C3-reversely prenylated product
Y205S
Y205T
mutant does not produce C3-regularly prenylated product
Y205V
mutant produces 33.0% C3-regularly prenylated product
Y205W
mutant does not produce C3-regularly prenylated product
E102Q
-
mutation reduces the production of tryprostatin B to undetectable amounts
-
G115A
-
no change in prenylation position of product
-
G115L
-
formation of tryprostatin B is abolished, formation of a new product occurs
-
G115T
-
0.4% of wild-type activity in formation of trytrostatin B. Mutant catalyzes the formation of a different product that carries a reverse prenyl moiety at the C-3 of the indole nucleus
-
H279F
-
70% of wild-type activity
-
Y205A
-
mutant produces 70.3% C3-regularly prenylated product
-
Y205C
-
mutant produces 39.5% C3-regularly prenylated product
-
Y205F
-
mutant produces 0.04% C3-regularly prenylated product
-
Y205L
-
mutant produces 56.4% C3-regularly prenylated product
-
Y205M
-
mutant produces 9.8% C3-regularly prenylated product
-