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2.5.1.113: [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase

This is an abbreviated version!
For detailed information about [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase, go to the full flat file.

Word Map on EC 2.5.1.113

Reaction

O-phospho-L-serine
+
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
=
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine
+
phosphate

Synonyms

CysM, Cysteine synthase, O-phosphoserine sulfhydrylase, O-phosphoserine-specific cysteine synthase, Rv1336

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.113 [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase

Crystallization

Crystallization on EC 2.5.1.113 - [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme bound to CysO sulfur-carrier protein, hanging drop vapor diffusion method, using 7-10% PEG (w/v) 4000, 0.1 M sodium citrate, pH 5.8, and 0.2 M ammonium acetate at 22°C
-
sitting drop vapor diffusion method, using 0.1 M Tris-HCl, pH 7.25-7.5, 0.1 M K2HPO4, 4.3 M NaCl