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2.5.1.16: spermidine synthase

This is an abbreviated version!
For detailed information about spermidine synthase, go to the full flat file.

Word Map on EC 2.5.1.16

Reaction

S-adenosyl 3-(methylsulfanyl)propylamine
+
putrescine
=
S-methyl-5'-thioadenosine
 +
spermidine

Synonyms

aminopropyltransferase, aminopropyltransferase spermidine synthase, AtSPDS1, AtSPDS2, MdSPDS1, PAPT, PgSPD, putrescine aminopropyltransferase, Spd synthase, SPDS, SPDS2, SPDSYN, spe-sdh, spe3, SpeE, spermidine synthase, spermidine synthase 1, spermidine synthetase, Spm synthase, SpSyn, Synpcc7942_0628, synthase, spermidine

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.16 spermidine synthase

Cloned

Cloned on EC 2.5.1.16 - spermidine synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as a His-tagged fusion protein
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 (lambdaDE3) Rosetta Oxford cells
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli, without the first 29 amino acids of the N-terminal extension
expression in Escherichiaa coli
expression in Pyrus communis
-
expression in transgenic Arabidopsis thaliana plants under control of the cauliflower mosaic virus 35S promoter
-
expression in transgenic Nicotiana tabacum
-
expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)
expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ACL5-1, DNA and amino acid sequence determination and analysis, ACL5-1 complements Spm biosynthesis in an enzyme-deficient yeast mutant, and ectopic expression of ACL5-1 in the Arabidopsis thaliana dwarf mutant acl5 allows recovery of the normal phenotype
gene ACL5-2, DNA and amino acid sequence determination and analysis
gene FSPD1, expression in Ipomoea batatas cv. Kokei 14 transgenic plants using the Agrobacterium tumefaciens strain EHA101 transfection system
-
gene MdSPDS1, expression in transgenic plants of Solanum lycopersicum cv. Microtom, that show significantly increased ascorbate peroxidase activity in MdSPDS1 transgenic plants compared to wild-type under saline conditions, phenotypes, overview
gene OeSPDS, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene PgSPD, DNA and amino acid sequence determination and analysis, expression of PgSPD under various environmental stresses at different time points using real time-PCR, overview
gene spdS, expression of the His-tagged and of GST-tagged enzyme in Escherichia coli strain M15, expression in CHO cells
-
gene spdS, overexpression in transgenic C57BL/6J mice
-
gene SPDS1, functional overexpression in transgenic Solanum lycopersicum cv. Micro-Tom plants via the Rhizobium radiobactor, strain GV2260, or Agrobacterium tumefaciens-mediated transformation method, quantitative real-time PCR enzyme expression analysis
gene SpdSyn, overexpression in transgenic Solanum lycopersicum plants, in leaves and fruits, under constitutive CaMV35S and fruit-ripening specific E8 promoters, phenotypes, overview
-
gene speE, sequence comparison, overexpression of the N-terminally His6-tagged enzyme in Escherichia coli strain SG13009 and in strain B834 for the selenomethionine-labeled enzyme
-
gene SPMS, DNA and amino acid sequence determination and analysis, SPMS complements Spm biosynthesis in an enzyme-deficient yeast mutant
overexpression in Pyrus communis
-
overexpression of soluble FLAG-tagged enzyme in HEK-293T cell cytoplasm
-
overexpression of the enzyme in strain clone DI700 episomes
-
recombinant overexpression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain SG13009