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2.5.1.16: spermidine synthase

This is an abbreviated version!
For detailed information about spermidine synthase, go to the full flat file.

Word Map on EC 2.5.1.16

Reaction

S-adenosyl 3-(methylsulfanyl)propylamine
+
putrescine
=
S-methyl-5'-thioadenosine
 +
spermidine

Synonyms

aminopropyltransferase, aminopropyltransferase spermidine synthase, AtSPDS1, AtSPDS2, MdSPDS1, PAPT, PgSPD, putrescine aminopropyltransferase, Spd synthase, SPDS, SPDS2, SPDSYN, spe-sdh, spe3, SpeE, spermidine synthase, spermidine synthase 1, spermidine synthetase, Spm synthase, SpSyn, Synpcc7942_0628, synthase, spermidine

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.16 spermidine synthase

Storage Stability

Storage Stability on EC 2.5.1.16 - spermidine synthase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, disrupted protoplasts lose more than 90% of activity within 1 week
-
-20°C, about 30% loss of activity in the final enzyme preparation within 1 month
-
-20°C, partially purified enzyme, several weeks with less than 20% loss of activity
-
-20ºC, 30% loss of activity in 1 month, after final filtration through Sephadex
-
-20ºC, partially purified enzyme, 0.1 M NaCl and 1% bovine serum albumin, loss of 50% of activity after 15 days
-
-20ºC, stable after the acetone precipitation
-
-20ºC, stable for at least 3 months after acetone precipitation
-
-80ºC, 20% glycerol, rapid loss of activity in pancreas preparations
-
-80ºC, 20% glycerol, stable for spleen tissue enzyme during storage and freeze-thawings
-
0-4°C, purified enzyme, 10-20% loss of activity within 6 months
-
0-4ºC, 10% loss of activity after several months
-
0°C, in buffer containing 3 M NaCl, 1 mM DTT and 0.05-0.25 mM decarboxylated S-adenosylmethionine, several months
0ºC, 30% loss of activity after 4 months
-
0ºC, 50% loss of activity in 2 weeks
-
4ºC, crude extracts, loss of activity within 24 hours
-