2 enzymes encoded by genes eutT and cobA, DNA sequence determination, analysis of several geno- and phenotypic strains, enzyme is expressed e.g. as fusion protein or to complement a deficient strain, overview
expression of the enzyme in C57/Bl6 mice using an adeno-associated virus vector, rAAV 2 and 8 vectors, with primer pairs specific to the cytomegalovirus enhancer/chicken beta-actin, CBAT, promoter, quantitative and semiquantitative expression analysis in murine liver, overview
from genomic DNA in pET20b for expression with N-terminal hexa-His tag in Escherichia coli strain BL21 (DE3) or B834 (DE3) for the selenomethionine derivative
gene cobA, recombinant overexpression of tagless, wild-type CobA and expression of His7-tagged mutant CobAs in Escherichia coli strain BL21(lambdaDE3) carrying a null allele of btuR, the cobA homologue in this bacterium
overexpression of wild-type and mutant enzymes in Escherichia coli using strain BL21(DE3) for the His-tagged variant, and several derivatives of strain DH5alpha, overview
QuickChangeXL site-directed mutagenesis performed on wild-type LrpduO in pTEV3 before expression of wild-type and variants in Escherichia coli BL21(DE3) with a rTEV protease-cleavable N-terminal hexa-His tag
without mitochondrial targeting sequence from plasmid pNL166 harbouring the coding sequence, native or with C-terminal octa-His tag into pTA925 to introduce a linker region necessary for expression in Escherichia coli BL21DE3 RIL and ATR-deficient Salmonella strain BE620, hATR in pCF13 (N-terminal octa-His tag) and pCF15 (C-terminal octa-His tag) for transformation and random mutagenesis in Escherichia coli mutator strain XL1-Red followed by expression in BE620
2 enzymes encoded by genes eutT and cobA, DNA sequence determination, analysis of several geno- and phenotypic strains, enzyme is expressed e.g. as fusion protein or to complement a deficient strain, overview
2 enzymes encoded by genes eutT and cobA, DNA sequence determination, analysis of several geno- and phenotypic strains, enzyme is expressed e.g. as fusion protein or to complement a deficient strain, overview