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0.27
Co2+
pH 8.0, 70°C, wild-type enzyme
0.00007 - 0.06
cob(I)alamin
0.000096
cob(I)inamide
-
-
0.0078 - 0.134
cob(II)alamin
0.0163
cob(II)inamide
wild type enzyme, pH and temperature not specified in the publication
0.11
Mg2+
pH 8.0, 70°C, wild-type enzyme
0.11
Mn2+
pH 8.0, 70°C, wild-type enzyme
additional information
additional information
-
0.0003
ATP
-
pH 8.0, 25°C, recombinant CobA mutant F91Y
0.0012
ATP
-
mutant F112Y, Co+ assay
0.0012
ATP
-
mutant F187A, Co+ assay
0.002
ATP
-
mutant F112W, Co+ assay
0.002
ATP
-
pH 8.0, 25°C, recombinant CobA mutant F91A/W93D
0.002
ATP
-
pH 8.0, 25°C, recombinant CobA mutant W93Y
0.0022
ATP
-
wild-type, Co+ assay
0.0026
ATP
-
mutant F112H, Co+ assay
0.0028
ATP
-
pH 8.0, 37°C
0.00287
ATP
mutant D218N, in presence of 0.05 mM cob(I)alamin
0.003
ATP
-
pH 8.0, 25°C, recombinant CobA mutant F91H
0.003
ATP
-
pH 8.0, 25°C, recombinant CobA mutant W93A
0.003
ATP
-
pH 8.0, 25°C, recombinant CobA mutant W93D
0.0031
ATP
-
mutant DELTAS183, Co+ assay
0.005
ATP
mutant T161I, in presence of 0.05 mM cob(I)alamin
0.005
ATP
-
wild-type, Co2+ assay
0.00523
ATP
mutant G97E, in presence of 0.05 mM cob(I)alamin
0.006
ATP
-
pH 8.0, 25°C, recombinant CobA mutant F91W/W93F
0.0063
ATP
-
recombinant enzyme variant 239K
0.0068
ATP
-
pH 8.0, 37°C, recombinant wild-type GST-tagged enzyme
0.0069
ATP
-
recombinant enzyme variant 239M
0.0069
ATP
native wild-type, in presence of 0.05 mM cob(I)alamin
0.007
ATP
-
pH 8.0, 25°C, recombinant wild-type CobA
0.00719
ATP
N-terminally octa-His tagged wild-type, in presence of 0.05 mM cob(I)alamin
0.00735
ATP
C-terminally octa-His tagged wild-type, in presence of 0.05 mM cob(I)alamin
0.0079
ATP
-
mutant F187A, Co2+ assay
0.00826
ATP
mutant K78R, in presence of 0.05 mM cob(I)alamin
0.009
ATP
-
pH 8.0, 25°C, recombinant CobA mutant F91A
0.009
ATP
-
pH 8.0, 25°C, recombinant CobA mutant F91W
0.0099
ATP
-
mutant DELTAS183, Co2+ assay
0.00997
ATP
mutant H183Y, in presence of 0.05 mM cob(I)alamin
0.01
ATP
-
pH 7.0, 37°C, recombinant enzyme
0.0104
ATP
-
mutant F112H, Co2+ assay
0.0124
ATP
mutant G63E, in presence of 0.05 mM cob(I)alamin
0.0141
ATP
mutant C119Y, in presence of 0.05 mM cob(I)alamin
0.0159
ATP
-
mutant F163A, Co+ assay
0.0172
ATP
mutant S68F, in presence of 0.05 mM cob(I)alamin
0.0198
ATP
-
pH 8.0, 37°C, recombinant full length wild-type enzyme
0.0203
ATP
mutant G97R, in presence of 0.05 mM cob(I)alamin
0.0205
ATP
mutant D64G, in presence of 0.05 mM cob(I)alamin
0.0254
ATP
wild type enzyme, pH and temperature not specified in the publication
0.026
ATP
-
pH 8.0, 25°C, recombinant CobA mutant W93H
0.03
ATP
-
pH 8.0, 25°C, recombinant CobA mutant W93F
0.0346
ATP
-
mutant F112A, Co+ assay
0.0459
ATP
mutant R76G, in presence of 0.05 mM cob(I)alamin
0.073
ATP
-
mutant F112W, Co2+ assay
0.0877
ATP
mutant S126L, in presence of 0.05 mM cob(I)alamin
0.094
ATP
-
mutant F112Y, Co2+ assay
0.0961
ATP
-
mutant F163A, Co2+ assay
0.11
ATP
pH 8.0, 70°C, wild-type enzyme, in presence of Mg2+
0.215
ATP
mutant F83S, in presence of 0.05 mM cob(I)alamin
0.32
ATP
-
pH 8.0, 37°C, recombinant GST-tagged mutant R191W
0.33
ATP
pH 8.0, 70°C, mutant R124A, in presence of Mg2+
0.00007
cob(I)alamin
-
mutant DELTAS183, Co+ assay
0.00011
cob(I)alamin
-
mutant F187A, Co+ assay
0.00013
cob(I)alamin
-
wild-type, Co+ assay
0.00019
cob(I)alamin
-
mutant F163A, Co+ assay
0.00037
cob(I)alamin
-
pH 8.0, 37°C, recombinant wild-type GST-tagged enzyme
0.00055
cob(I)alamin
-
mutant F112Y, Co+ assay
0.00077
cob(I)alamin
-
mutant F112W, Co+ assay
0.001
cob(I)alamin
-
pH 8.0, 37°C
0.001
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant F91Y
0.0012
cob(I)alamin
-
recombinant enzyme variant 239K
0.00158
cob(I)alamin
N-terminally octa-His tagged wild-type, in presence of 0.5 mM ATP
0.0016
cob(I)alamin
-
recombinant enzyme variant 239M
0.0016
cob(I)alamin
C-terminally octa-His tagged wild-type, in presence of 0.5 mM ATP
0.0016
cob(I)alamin
native wild-type, in presence of 0.5 mM ATP
0.00172
cob(I)alamin
mutant D218N, in presence of 0.5 mM ATP
0.00186
cob(I)alamin
mutant S68F, in presence of 0.5 mM ATP
0.0019
cob(I)alamin
-
mutant F112A, Co+ assay
0.00194
cob(I)alamin
mutant D64G, in presence of 0.5 mM ATP
0.002
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant W93D
0.002
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant W93H
0.00224
cob(I)alamin
mutant G63E, in presence of 0.5 mM ATP
0.00225
cob(I)alamin
mutant K78R, in presence of 0.5 mM ATP
0.00252
cob(I)alamin
mutant G97E, in presence of 0.5 mM ATP
0.00271
cob(I)alamin
mutant T161I, in presence of 0.5 mM ATP
0.0028
cob(I)alamin
-
mutant F112H, Co+ assay
0.003
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant F91A/W93D
0.003
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant F91H
0.00303
cob(I)alamin
mutant G97R, in presence of 0.5 mM ATP
0.004
cob(I)alamin
-
at pH 7.0 and 37°C
0.004
cob(I)alamin
-
pH 8.0, 25°C, recombinant wild-type CobA
0.0041
cob(I)alamin
-
pH 7.0, 37°C, recombinant enzyme
0.0045
cob(I)alamin
-
pH 8.0, 37°C, recombinant full length wild-type enzyme
0.005
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant F91A
0.005
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant F91W
0.00513
cob(I)alamin
mutant C119Y, in presence of 0.5 mM ATP
0.0052
cob(I)alamin
-
pH 8.0, 37°C
0.00713
cob(I)alamin
mutant H183Y, in presence of 0.5 mM ATP
0.009
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant F91W/W93F
0.00909
cob(I)alamin
mutant R76G, in presence of 0.5 mM ATP
0.011
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant W93A
0.012
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant W93F
0.0143
cob(I)alamin
mutant S126L, in presence of 0.5 mM ATP
0.016
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant W93Y
0.0302
cob(I)alamin
mutant F83S, in presence of 0.5 mM ATP
0.06
cob(I)alamin
-
pH 8.0, 37°C, recombinant GST-tagged mutant R191W
0.0078
cob(II)alamin
-
wild-type, Co2+ assay
0.015
cob(II)alamin
-
mutant F187A, Co2+ assay
0.016
cob(II)alamin
-
mutant DELTAS183, Co2+ assay
0.028
cob(II)alamin
-
mutant F112W, Co2+ assay
0.034
cob(II)alamin
-
mutant F112Y, Co2+ assay
0.053
cob(II)alamin
-
mutant F112H, Co2+ assay
0.134
cob(II)alamin
-
mutant F163A, Co2+ assay
0.003
Cobalamin
pH 8.0, 70°C, wild-type enzyme, in presence of Mg2+
0.004
Cobalamin
pH 8.0, 70°C, mutant R124A, in presence of Mg2+
0.01
cyanocob(I)alamin
-
-
0.01
cyanocob(I)alamin
-
pH 8.0, 37°C, i.e. vitamin B12, reduced form
0.14
dATP
-
0.14
dATP
pH 8.0, 70°C, wild-type enzyme, in presence of Mg2+
0.003
hydroxocobalamin
-
0.003
hydroxocobalamin
+/- 0.0004
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
steady-state kinetics
-
additional information
additional information
elevated for either ATP or cobalamin or both substrates in case of mutants which show decreased adenosylcobalamin synthesis activity that can be partly corrected by increased hydroxycobalamin concentration
-
additional information
additional information
-
elevated for either ATP or cobalamin or both substrates in case of mutants which show decreased adenosylcobalamin synthesis activity that can be partly corrected by increased hydroxycobalamin concentration
-
additional information
additional information
large changes in KM for both substrates for mutants R76G, F83S and S126L
-
additional information
additional information
-
large changes in KM for both substrates for mutants R76G, F83S and S126L
-
additional information
additional information
wild-type kinetics by mutants G97E, C119Y, T161I, and H183Y
-
additional information
additional information
-
wild-type kinetics by mutants G97E, C119Y, T161I, and H183Y
-
additional information
additional information
-
wild-type: KM for CTP and UTP increased relative to ATP
-
additional information
additional information
-
wild-type: KM for GTP and ITP 530-13000fold increased relative to ATP
-
additional information
additional information
-
kinetics for wild-type and mutants enzymes from two different assay methods, overview
-