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0.00041 - 297.6
cob(I)alamin
0.00067 - 0.26
cob(II)alamin
0.0077
cob(II)inamide
wild type enzyme, pH and temperature not specified in the publication
additional information
2'-deoxy-ATP
0.00038
ATP
-
mutant F112H, Co+ assay
0.0005
ATP
-
mutant F112H, Co2+ assay
0.0016
ATP
-
mutant F112Y, Co+ assay
0.0018
ATP
-
mutant F112W, Co+ assay
0.0023
ATP
-
mutant F112Y, Co2+ assay
0.0028
ATP
-
mutant F112W, Co2+ assay
0.0038
ATP
-
mutant S129A
0.0056
ATP
-
mutant F112A, Co+ assay
0.0062
ATP
-
mutant R128K
0.0067
ATP
wild type enzyme, pH and temperature not specified in the publication
0.008
ATP
-
pH 8.0, 25°C, recombinant CobA mutant F91A/W93D
0.0084
ATP
-
mutant R132K
0.01
ATP
-
pH 8.0, 25°C, recombinant CobA mutant F91W/W93F
0.01
ATP
-
pH 8.0, 25°C, recombinant CobA mutant W93F
0.014
ATP
-
mutant F187A, Co2+ assay
0.015
ATP
-
mutant F163A, Co+ assay
0.015
ATP
-
mutant F163A, Co2+ assay
0.017
ATP
-
mutant DELTAS183, Co2+ assay
0.02
ATP
-
pH 8.0, 25°C, recombinant CobA mutant F91A
0.02
ATP
-
pH 8.0, 25°C, recombinant CobA mutant F91H
0.021
ATP
-
mutant F187A, Co+ assay
0.023
ATP
-
mutant DELTAS183, Co+ assay
0.026
ATP
-
wild-type, Co+ assay
0.029
ATP
-
wild-type, Co2+ assay
0.03
ATP
-
pH 7.0, 37°C, recombinant enzyme
0.04
ATP
-
pH 8.0, 25°C, recombinant CobA mutant W93A
0.05
ATP
-
pH 8.0, 25°C, recombinant CobA mutant F91Y
0.05
ATP
-
pH 8.0, 25°C, recombinant CobA mutant W93D
0.07
ATP
-
pH 8.0, 25°C, recombinant CobA mutant W93H
0.07
ATP
mutant enzyme D110N, pH and temperature not specified in the publication
0.08
ATP
-
pH 8.0, 25°C, recombinant CobA mutant F91W
0.08
ATP
-
pH 8.0, 25°C, recombinant wild-type CobA
0.096
ATP
-
mutant D35E/R128K
0.1
ATP
-
pH 8.0, 25°C, recombinant CobA mutant W93Y
0.1
ATP
mutant enzyme R200K, pH and temperature not specified in the publication
0.14
ATP
pH 8.0, 70°C, mutant R124A, in presence of Mg2+
0.17
ATP
mutant enzyme K98Q, pH and temperature not specified in the publication
0.18
ATP
mutant enzyme E207Q, pH and temperature not specified in the publication
0.23
ATP
pH 8.0, 70°C, wild-type enzyme, in presence of Mg2+
0.24
ATP
wild-type enzyme, pH and temperature not specified in the publication
0.27
ATP
mutant enzyme T88V, pH and temperature not specified in the publication
0.41
ATP
-
mutant D35N/R128K
9
ATP
-
mutant D35R/R128D, at subsaturating concentrations of 30 mM ATP and 0.02 mM cob(I)alamin
166.2
ATP
N-terminally octa-His tagged wild-type
297.6
ATP
C-terminally octa-His tagged wild-type
0.00041
cob(I)alamin
-
mutant R132K
0.00042
cob(I)alamin
-
mutant F112H, Co+ assay
0.00065
cob(I)alamin
-
mutant D35R/R128D, at subsaturating concentrations of ATP 30 mM ATP and 0.02 mM cob(I)alamin
0.0017
cob(I)alamin
-
mutant F112Y, Co+ assay
0.002
cob(I)alamin
-
mutant F112W, Co+ assay
0.0024
cob(I)alamin
-
mutant D35E/R128K
0.004
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant F91W/W93F
0.0066
cob(I)alamin
-
mutant F112A, Co+ assay
0.0068
cob(I)alamin
-
mutant D35N
0.009
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant W93F
0.009
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant W93Y
0.01
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant F91A/W93D
0.011
cob(I)alamin
-
pH 8.0, 37°C, recombinant GST-tagged mutant R191W
0.011
cob(I)alamin
-
mutant R128K
0.014
cob(I)alamin
-
mutant D35N/R128K
0.015
cob(I)alamin
-
mutant S129A
0.015
cob(I)alamin
-
mutant F163A, Co+ assay
0.018
cob(I)alamin
-
mutant F187A, Co+ assay
0.02
cob(I)alamin
-
mutant DELTAS183, Co+ assay
0.02
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant F91A
0.02
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant F91H
0.024
cob(I)alamin
-
wild-type, Co+ assay
0.037
cob(I)alamin
-
pH 8.0, 37°C, recombinant wild-type GST-tagged enzyme
0.05
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant F91Y
0.05
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant W93A
0.05
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant W93D
0.06
cob(I)alamin
-
pH 7.0, 37°C, recombinant enzyme
0.06
cob(I)alamin
-
at pH 7.0 and 37°C
0.08
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant F91W
0.08
cob(I)alamin
-
pH 8.0, 25°C, recombinant CobA mutant W93H
0.08
cob(I)alamin
-
pH 8.0, 25°C, recombinant wild-type CobA
19.2
cob(I)alamin
mutant K78R
29.4
cob(I)alamin
mutant G63E
31.8
cob(I)alamin
mutant D64G
34.8
cob(I)alamin
mutant S126L
43.8
cob(I)alamin
mutant D218N
46.8
cob(I)alamin
mutant S68F
48
cob(I)alamin
mutant G97R
124.2
cob(I)alamin
mutant F83S
137.4
cob(I)alamin
mutant T161I
154.8
cob(I)alamin
mutant R76G
166.2
cob(I)alamin
N-terminally octa-His tagged wild-type
169.8
cob(I)alamin
mutant G97E
169.8
cob(I)alamin
mutant H183Y
243.6
cob(I)alamin
mutant C119Y
285
cob(I)alamin
native wild-type
297.6
cob(I)alamin
C-terminally octa-His tagged wild-type
0.00067
cob(II)alamin
-
mutant F112H, Co2+ assay
0.0027
cob(II)alamin
-
mutant F112Y, Co2+ assay
0.0032
cob(II)alamin
-
mutant F112W, Co2+ assay
0.015
cob(II)alamin
-
mutant F187A, Co2+ assay
0.018
cob(II)alamin
-
mutant DELTAS183, Co2+ assay
0.027
cob(II)alamin
-
mutant F163A, Co2+ assay
0.038
cob(II)alamin
-
wild-type, Co2+ assay
0.05
cob(II)alamin
mutant enzyme D110N, pH and temperature not specified in the publication
0.06
cob(II)alamin
mutant enzyme R200K, pH and temperature not specified in the publication
0.18
cob(II)alamin
mutant enzyme K98Q, pH and temperature not specified in the publication
0.21
cob(II)alamin
wild-type enzyme, pH and temperature not specified in the publication
0.22
cob(II)alamin
mutant enzyme E207Q, pH and temperature not specified in the publication
0.26
cob(II)alamin
mutant enzyme T88V, pH and temperature not specified in the publication
0.11
Cobalamin
pH 8.0, 70°C, mutant R124A, in presence of Mg2+
0.18
Cobalamin
pH 8.0, 70°C, wild-type enzyme, in presence of Mg2+
0.11
dATP
-
0.11
dATP
pH 8.0, 70°C, wild-type enzyme, in presence of Mg2+
0.18
hydroxocobalamin
-
0.18
hydroxocobalamin
+/- 0.01
additional information
2'-deoxy-ATP
-
kcat/KM: 3300 1/M*s
additional information
ATP
C-terminally octa-His tagged wild-type, kcat/KM is 0.67 per microM and min
additional information
ATP
-
C-terminally octa-His tagged wild-type, kcat/KM is 0.67 per microM and min
additional information
ATP
-
kcat/KM: 12000 1/M*s
additional information
ATP
mutant C119Y, kcat/KM is 0.29 per microM and min
additional information
ATP
-
mutant C119Y, kcat/KM is 0.29 per microM and min
additional information
ATP
mutant D218N, kcat/KM is 0.25 per microM and min
additional information
ATP
-
mutant D218N, kcat/KM is 0.25 per microM and min
additional information
ATP
-
mutant D35E/R128K, kcat/KM: 23 1/M*s
additional information
ATP
-
mutant D35N, kcat/KM: 52 1/M*s
additional information
ATP
-
mutant D35N/R128K, kcat/KM: 32 1/M*s
additional information
ATP
-
mutant D35R/R128D, kcat/KM: 0.014 1/M*s, at subsaturating concentrations of 30 mM ATP and 0.02 mM cob(I)alamin
additional information
ATP
mutant D64G, kcat/KM is 0.026 per microM and min
additional information
ATP
-
mutant D64G, kcat/KM is 0.026 per microM and min
additional information
ATP
mutant F83S, kcat/KM is 0.010 per microM and min
additional information
ATP
-
mutant F83S, kcat/KM is 0.010 per microM and min
additional information
ATP
mutant G63E, kcat/KM is 0.039 per microM and min
additional information
ATP
-
mutant G63E, kcat/KM is 0.039 per microM and min
additional information
ATP
mutant G97E, kcat/KM is 0.46 per microM and min
additional information
ATP
-
mutant G97E, kcat/KM is 0.46 per microM and min
additional information
ATP
mutant G97R, kcat/KM is 0.039 per microM and min
additional information
ATP
-
mutant G97R, kcat/KM is 0.039 per microM and min
additional information
ATP
mutant H183Y, kcat/KM is 0.28 per microM and min
additional information
ATP
-
mutant H183Y, kcat/KM is 0.28 per microM and min
additional information
ATP
mutant K78R, kcat/KM is 0.039 per microM and min
additional information
ATP
-
mutant K78R, kcat/KM is 0.039 per microM and min
additional information
ATP
-
mutant R128K, kcat/KM: 1800 1/M*s
additional information
ATP
-
mutant R132K, kcat/KM: 39 1/M*s
additional information
ATP
mutant R76G, kcat/KM is 0.056 per microM and min
additional information
ATP
-
mutant R76G, kcat/KM is 0.056 per microM and min
additional information
ATP
mutant S126L, kcat/KM is 0.007 per microM and min
additional information
ATP
-
mutant S126L, kcat/KM is 0.007 per microM and min
additional information
ATP
-
mutant S129A, kcat/KM: 4500 1/M*s
additional information
ATP
mutant S68F, kcat/KM is 0.045 per microM and min
additional information
ATP
-
mutant S68F, kcat/KM is 0.045 per microM and min
additional information
ATP
mutant T161I, kcat/KM is 0.46 per microM and min
additional information
ATP
-
mutant T161I, kcat/KM is 0.46 per microM and min
additional information
ATP
N-terminally octa-His tagged wild-type, kcat/KM is 0.39 per microM and min
additional information
ATP
-
N-terminally octa-His tagged wild-type, kcat/KM is 0.39 per microM and min
additional information
ATP
native wild-type, kcat/KM is 0.69 per microM and min
additional information
ATP
-
native wild-type, kcat/KM is 0.69 per microM and min
additional information
cob(I)alamin
C-terminally octa-His tagged wild-type, kcat/KM is 3.10 per microM and min
additional information
cob(I)alamin
-
C-terminally octa-His tagged wild-type, kcat/KM is 3.10 per microM and min
additional information
cob(I)alamin
-
kcat/KM: 180000 1/M*s
additional information
cob(I)alamin
mutant C119Y, kcat/KM is 0.79 per microM and min
additional information
cob(I)alamin
-
mutant C119Y, kcat/KM is 0.79 per microM and min
additional information
cob(I)alamin
mutant D218N, kcat/KM is 0.42 per microM and min
additional information
cob(I)alamin
-
mutant D218N, kcat/KM is 0.42 per microM and min
additional information
cob(I)alamin
-
mutant D35E/R128K, kcat/KM: 900 1/M*s
additional information
cob(I)alamin
-
mutant D35N, kcat/KM: 2800 1/M*s
additional information
cob(I)alamin
-
mutant D35N/R128K, kcat/KM: 3800 1/M*s
additional information
cob(I)alamin
-
mutant D35R/R128D, kcat/KM: 5.2 1/M*s, at subsaturating concentrations of 30 mM ATP and 0.02 mM cob(I)alamin
additional information
cob(I)alamin
mutant D64G, kcat/KM is 0.27 per microM and min
additional information
cob(I)alamin
-
mutant D64G, kcat/KM is 0.27 per microM and min
additional information
cob(I)alamin
mutant F83S, kcat/KM is 0.07 per microM and min
additional information
cob(I)alamin
-
mutant F83S, kcat/KM is 0.07 per microM and min
additional information
cob(I)alamin
mutant G63E, kcat/KM is 0.22 per microM and min
additional information
cob(I)alamin
-
mutant G63E, kcat/KM is 0.22 per microM and min
additional information
cob(I)alamin
mutant G97E, kcat/KM is 0.94 per microM and min
additional information
cob(I)alamin
-
mutant G97E, kcat/KM is 0.94 per microM and min
additional information
cob(I)alamin
mutant G97R, kcat/KM is 0.26 per microM and min
additional information
cob(I)alamin
-
mutant G97R, kcat/KM is 0.26 per microM and min
additional information
cob(I)alamin
mutant H183Y, kcat/KM is 0.40 per microM and min
additional information
cob(I)alamin
-
mutant H183Y, kcat/KM is 0.40 per microM and min
additional information
cob(I)alamin
mutant K78R, kcat/KM is 0.14 per microM and min
additional information
cob(I)alamin
-
mutant K78R, kcat/KM is 0.14 per microM and min
additional information
cob(I)alamin
-
mutant R128K, kcat/KM: 12000 1/M*s
additional information
cob(I)alamin
-
mutant R132K, kcat/KM: 55 1/M*s
additional information
cob(I)alamin
mutant R76G, kcat/KM is 0.28 per microM and min
additional information
cob(I)alamin
-
mutant R76G, kcat/KM is 0.28 per microM and min
additional information
cob(I)alamin
mutant S126L, kcat/KM is 0.04 per microM and min
additional information
cob(I)alamin
-
mutant S126L, kcat/KM is 0.04 per microM and min
additional information
cob(I)alamin
-
mutant S129A, kcat/KM: 65000 1/M*s
additional information
cob(I)alamin
mutant S68F, kcat/KM is 0.42 per microM and min
additional information
cob(I)alamin
-
mutant S68F, kcat/KM is 0.42 per microM and min
additional information
cob(I)alamin
mutant T161I, kcat/KM is 0.85 per microM and min
additional information
cob(I)alamin
-
mutant T161I, kcat/KM is 0.85 per microM and min
additional information
cob(I)alamin
N-terminally octa-His tagged wild-type, kcat/KM is 1.75 per microM and min
additional information
cob(I)alamin
-
N-terminally octa-His tagged wild-type, kcat/KM is 1.75 per microM and min
additional information
cob(I)alamin
native wild-type, kcat/KM is 2.97 per microM and min
additional information
cob(I)alamin
-
native wild-type, kcat/KM is 2.97 per microM and min
additional information
CTP
-
kcat/KM: 0.38 1/M*s
additional information
GTP
-
kcat/KM: 18 1/M*s
additional information
ITP
-
kcat/KM: 0.65 1/M*s
additional information
additional information
-
recombinant truncated enzyme versions
-
additional information
additional information
-
adenosylation of cobalamin and cobinamide (lacking dimethylbenzimidazole moiety) at similar rates
-
additional information
additional information
kcat of N-terminal octa-His tagged enzyme is 58% of native or C-terminal tagged enzyme possibly due to interference of N-terminal tag with base-off transition of adenosylcobalamin by the enzyme
-
additional information
additional information
-
kcat of N-terminal octa-His tagged enzyme is 58% of native or C-terminal tagged enzyme possibly due to interference of N-terminal tag with base-off transition of adenosylcobalamin by the enzyme
-
additional information
additional information
mutations in conserved regions E84-S94 and R186-R194 lead to abolished enzymatic activity and support their implication in the enzymes active site
-
additional information
additional information
-
mutations in conserved regions E84-S94 and R186-R194 lead to abolished enzymatic activity and support their implication in the enzymes active site
-
additional information
additional information
-
wild-type, decreased kcat for CTP and UTP relative to ATP
-
additional information
additional information
-
wild-type, kcat for GTP and ITP is not decreased relative to ATP
-
additional information
UTP
-
kcat/KM: 0.048 1/M*s