2.5.1.18: glutathione transferase
This is an abbreviated version!
For detailed information about glutathione transferase, go to the full flat file.
Word Map on EC 2.5.1.18
-
2.5.1.18
-
catalase
-
dismutase
-
sod
-
pull-down
-
malondialdehyde
-
carcinogenesis
-
detoxify
-
hepatocytes
-
smoke
-
isoenzymes
-
cyp1a1
-
smoking
-
case-control
-
electrophilic
-
epoxide
-
aquatic
-
wistar
-
genotoxic
-
insecticide
-
chemopreventive
-
gill
-
hepatocarcinogenesis
-
acetylcholinesterase
-
polycyclic
-
pesticide
-
hydroperoxide
-
thiobarbituric
-
hepatotoxicity
-
tbars
-
ache
-
albino
-
metallothioneins
-
cigarette
-
benzoapyrene
-
phenobarbital
-
drug-metabolizing
-
preneoplastic
-
alt
-
aflatoxin
-
gssg
-
hepatoprotective
-
schistosoma
-
comet
-
carboxylesterase
-
mussel
-
udp-glucuronosyltransferase
-
methylation-specific
-
gsh-px
-
organophosphate
-
drug development
-
medicine
-
cyp2d6
-
biotechnology
-
analysis
- 2.5.1.18
- catalase
- dismutase
- sod
-
pull-down
- malondialdehyde
- carcinogenesis
-
detoxify
- hepatocytes
-
smoke
- isoenzymes
- cyp1a1
-
smoking
-
case-control
-
electrophilic
- epoxide
-
aquatic
- wistar
-
genotoxic
-
insecticide
-
chemopreventive
- gill
-
hepatocarcinogenesis
- acetylcholinesterase
-
polycyclic
-
pesticide
- hydroperoxide
-
thiobarbituric
-
hepatotoxicity
-
tbars
-
ache
-
albino
- metallothioneins
-
cigarette
-
benzoapyrene
- phenobarbital
-
drug-metabolizing
-
preneoplastic
-
alt
- aflatoxin
- gssg
-
hepatoprotective
- schistosoma
- comet
- carboxylesterase
- mussel
-
udp-glucuronosyltransferase
-
methylation-specific
- gsh-px
-
organophosphate
- drug development
- medicine
- cyp2d6
- biotechnology
- analysis
Reaction
Synonyms
2-hydroxychromene-2-carboxylic acid isomerase, 26GST, adGSTD4-4, agGSTe2, allergen Der p 2, allergen Der p 8, alpha class glutathione transferase, Alpha class GST, alpha GST, alpha-class glutathione transferase, asGST5.5, AtGSTF2, AtuGSTH1-1, BaeAB, beta-etherase, bmGSTD, BphK, CgGSTM1, CgGSTM2, cGST, Chi class GST, Chi GST, class mu glutathione S-transferase, delta class glutathione transferase, delta-class glutathione S-transferase, Delta-class glutathione transferase, EC 1.8.6.1, EC 2.5.1.12, EC 2.5.1.13, EC 2.5.1.14, EC 4.4.1.7, EGST, epsilon class GST, Epsilon glutathione S-transferase, Epsilon glutathione transferase, Epsilon GST, epsilon-class glutathione S-transferase, epsilon-class glutathione transferase, GHR, glutathione S-alkyl transferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase, glutathione S-transferase 2, glutathione S-transferase A1-1, glutathione S-transferase A3-3, glutathione S-transferase AdFSTD3-3, glutathione S-transferase I, glutathione S-transferase omega 1, glutathione S-transferase omega 2, glutathione S-transferase P1-1, glutathione S-transferase pi, glutathione S-transferase Pi-1, glutathione S-transferase X, glutathione transferase, glutathione transferase A1-1, glutathione transferase A4-4, glutathione transferase M1-1, glutathione transferase M2-2, glutathione transferase Omega 3S, glutathione transferase omega-1, glutathione transferase P1-1, glutathione transferase Pi, glutathione transferase T1-1, glutathione transferase zeta, glutathione transferase zeta 1, glutathione transferase Zeta 1-1, glutathione transferase zeta1-1, glutathione transferase-like protein, glutathione-S-transferas, glutathione-S-transferase, glutathione-S-transferase pi, glutathione-transferase, GmGSTU4-4, GSH S-transferase, GSH transferase, GSH transferase homologue, GSH-S transferase rho, GSHTase-P, GST, GST A1-1, GST A2-2, GST A3-3, GST A4-4, GST adgstD4-4, GST alpha, GST Delta 2, GST I, GST II, GST III, GST IV, GST M1-1, GST M2-2, GST M4-4, GST M5-5, GST mu, GST O1-1, GST P1-1, GST pi, GST T1-1, GST Tau, GST Tau19, GST Z1-1, GST-1, GST-2, GST-26, GST-3, GST-Acr, GST-OCX-32, GST-T, GST-theta, GST1, GST1b, GST2, GST20, GST3, GST4, GST5, GST5118, GST7, GST83044, Gsta, GSTA1, GSTA1-1, GSTA2-2, GSTA3, GSTA3-3, GSTA4, GSTA4-4, GSTA4L, GSTA5, GSTA5-5, GSTalpha1, GSTD, GSTD1, GSTD10, GSTd14, GSTD2, GSTD4, GSTD4-4, GSTE1, GSTE2, GSTE3, GSTE4, GSTE5, GSTE6, GSTE7, GSTE8, GSTF12-1, GSTF12-2, GSTF3, GSTF5, GSTF9, GSTFuA1, GSTk, GSTK1, GSTL1, GSTL2, GSTL3, Gstm, GSTm09, GSTM1, GSTM1-1, GSTM2, GSTM2-2, GSTM3, GSTM3-3, GSTM4, GSTM4-4, GSTM5, GSTM5-5, GSTmu, GSTO, GSTO1, GSTO1-1, GSTO2, GSTO2-2, GstO2A, GstO2B, GSTO3, GSTO3S, GSTO4, GSTO7, GSTP, GSTP 1-1, GSTP-1, GSTP1, GSTP1-1, Gstr1, GSTrho, GSTS, GSTS1, GSTS1-1, GSTS2, GSTS3, GSTT, GSTT1, GSTT1-1, Gstt1a, GSTT2, GSTT2-2, GSTT2B-2B, GSTT4, GSTT4L, GSTU1, GSTU10, GSTU10-10, GSTU11, GSTU12, GSTU13, GSTU13-1, GSTU14, GSTU16, GSTU17, GSTU18, GSTU19, GSTU2, GSTU2-2, GSTU21, GSTU24, GSTU24-1, GSTU25, GSTU26, GSTU28, GSTU3, GSTU4, GSTU45, GSTU5, GSTU6, GSTU7, GSTU8, GSTU9, GSTZ, Gstz1, GSTZ1-1, GTT1.2, HCCA isomerase, Hematopoietic prostaglandin D synthase, hGSTA-3, hGSTA1-1, hGSTZ1-1, kappa class glutathione transferase, kappa class GSH transferase, Kappa class GST, KKSG9, lambda glutathione transferase, MAAI, MGST1, MGST2, MGST3, Mgst3a, Mgst3b, microsomal glutathione transferase 1, microsomal glutathione transferase-1, More, mtMGST1, mu class glutathione S-transferase, Mu class GST, mu glutathione transferase, Mu GST, mu-class glutathione S-transferase, mu-class glutathione S-transferase1, Mu-class GST, Mu-GST, nu-class glutathione transferase, Omega class GST, omega glutathione S-transferase, omega glutathione transferase, omega-class glutathione S-transferase, omega-class glutathione S-transferase 2, pGSTA1, phi class glutathione transferase, Pi class GST, pm-GSTR1, PmGST, PtGSTU1, PvGSTF1-1, PvGSTU2-2, PvGSTU3-3, rho class glutathione S-transferase, rho-GST, RX: glutathione R-transferase, RX:glutathione R-transferase, S-(hydroxyalkyl)glutathione lyase, Saro_2873/Saro_2872, selenium-containing glutathione transferase zeta1-1, seleno-hGSTZ1-1, SGST26.5, sigma class glutathione transferase, Sigma class GST, sigma glutathione S-transferase, sigma-class glutathione S-transferase, sigma-class GST, SIGST, Sj26GST, sjGST, sll1545, ssGST3, ssGST5, tau class glutathione S-transferase, tau class glutathione transferase, tau class GST, tau class GSTU4-4, TDR1, theta class glutathione S-transferase, theta class glutathione transferase T1-1, theta class GST, theta-class glutathione transferase, thiol-dependent reductase I, Ure2p mutant A122C, Ure2pB1, Xi class glutathione transferase, Ya-GST, YghU, zeta class glutathione S-transferase, Zeta class GST
ECTree
2.5.1.18 glutathione transferaseAdvanced search results
results (
results (KM Value
KM Value on EC 2.5.1.18 - glutathione transferase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.084
2-hydroxychromene-2-carboxylic acid
-
pH 7.0, 25°C, recombinant enzyme
4.1
2-hydroxyethyl disulfide
-
pH 6.5, 37°C, recombinant AtuGSTH1-1
0.232
3,4-dinitrobenzanilide
in phosphate buffered saline, pH 7.4, at 37°C
2.24
acetyl-gamma-Glu-Cys-Gly
mutant enzyme I104V/A113V, at pH 7.4 and 25°C
0.01461
alpha-O-(beta-methylumbelliferyl)acetovanillone
wild type enzyme, pH and temperature not specified in the publication
0.207
S-phenylacetylglutathione
isoform GSTO4, at pH 6.5 and 20°C
0.138
trans-o-hydroxybenzylidene pyruvic acid
-
pH 7.0, 25°C, recombinant enzyme
0.023
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid
-
pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c
0.027
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid
-
pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c
0.029
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid
-
pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1d-1d
0.044
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid
-
pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1c-1c
0.096
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid
-
pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1b-1b
0.128
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid
-
pH 7.4, 37°C, enzyme variant glutathione transferase Zeta 1a-1a
0.319
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid
-
pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c
0.417
(+)-2-bromo-3-(4-nitrophenyl)propanoic acid
-
pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c
4.2
(R)-styrene-7,8-oxide
-
mutant enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
4.7
(R)-styrene-7,8-oxide
-
wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
8.8
(R)-styrene-7,8-oxide
-
mutant enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
0.8
(R,R)-1-phenylpropylene oxide
-
wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
0.9
(R,R)-1-phenylpropylene oxide
-
wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
2.4
(R,R)-1-phenylpropylene oxide
-
wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
0.035
(R,R)-trans-stilbene oxide
-
wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
0.06
(R,R)-trans-stilbene oxide
-
wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
0.1
(R,R)-trans-stilbene oxide
-
wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
1.3
(S)-styrene-7,8-oxide
-
mutant enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
1.6
(S)-styrene-7,8-oxide
-
mutant enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
4.9
(S)-styrene-7,8-oxide
-
wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
2.2
(S,S)-1-phenylpropylene oxide
-
wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
2.4
(S,S)-1-phenylpropylene oxide
-
wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
3.8
(S,S)-1-phenylpropylene oxide
-
wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
0.08
(S,S)-trans-stilbene oxide
-
wild type enzyme G112C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
0.11
(S,S)-trans-stilbene oxide
-
wild type enzyme, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
0.12
(S,S)-trans-stilbene oxide
-
wild type enzyme I10C, at 30°C in 250 mM Tris-HCl buffer, pH 7.2
0.6
1,3-Bis-(2-chloroethyl)-1-nitrosourea
-
pH 7.4, 37°C, recombinant mutant F2:1215
0.6
1,3-Bis-(2-chloroethyl)-1-nitrosourea
-
pH 7.4, 37°C, recombinant mutant F2:1215
0.71
1,3-Bis-(2-chloroethyl)-1-nitrosourea
-
pH 7.5, 28°C, GSH-S transferase rho
0.8
1,3-Bis-(2-chloroethyl)-1-nitrosourea
-
pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1
1
1,3-Bis-(2-chloroethyl)-1-nitrosourea
-
pH 7.4, 37°C, recombinant wild-type isozyme GST-T1-1
0.0067
1-chloro-2,4-dinitrobenzene
recombinant His-tagged wild-type enzyme, pH and temperature not specified in the publication
0.0084
1-chloro-2,4-dinitrobenzene
recombinant His-tagged mutant R113A, pH and temperature not specified in the publication
0.0088
1-chloro-2,4-dinitrobenzene
recombinant detagged wild-type enzyme, pH and temperature not specified in the publication
0.019
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 25°C, recombinant wild-type enzyme
0.03
1-chloro-2,4-dinitrobenzene
-
recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication
0.05
1-chloro-2,4-dinitrobenzene
-
recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication
0.0728
1-chloro-2,4-dinitrobenzene
recombinant His-tagged mutant F108G, pH and temperature not specified in the publication
0.08
1-chloro-2,4-dinitrobenzene
-
recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication
0.1033
1-chloro-2,4-dinitrobenzene
-
pH and temperature not specified in the publication
0.14
1-chloro-2,4-dinitrobenzene
pH 6.5, 30°C, recombinant GSTU4-4 mutant P49A
0.156
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU10, at pH 6.5 and 25°C
0.158
1-chloro-2,4-dinitrobenzene
pH 6.5, 30°C, recombinant wild-type GSTU4-4
0.19
1-chloro-2,4-dinitrobenzene
-
recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication
0.201
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU1, at pH 6.5 and 25°C
0.26
1-chloro-2,4-dinitrobenzene
pH 6.5, 30°C, recombinant GSTU4-4 mutant D48A
0.26
1-chloro-2,4-dinitrobenzene
mutant enzyme S11A, at pH 8.0 and 30°C
0.29
1-chloro-2,4-dinitrobenzene
isoform GSTU45, pH not specified in the publication, at 30°C
0.305
1-chloro-2,4-dinitrobenzene
mutant enzyme F136A, at pH 7.5, temperature not specified in the publication
0.35
1-chloro-2,4-dinitrobenzene
mutant enzyme Y215F, in 0.1 M phosphate buffer pH 6.5
0.35
1-chloro-2,4-dinitrobenzene
-
recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication
0.4
1-chloro-2,4-dinitrobenzene
-
isoform GSTFuA1, at pH 7.5, temperature not specified in the publication
0.402
1-chloro-2,4-dinitrobenzene
wild type enzyme, at pH 7.5, temperature not specified in the publication
0.41
1-chloro-2,4-dinitrobenzene
-
recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication
0.412
1-chloro-2,4-dinitrobenzene
mutant enzyme N22A, pH and temperature not specified in the publication
0.42
1-chloro-2,4-dinitrobenzene
mutant enzyme F119A, at pH 8.0 and 30°C
0.44
1-chloro-2,4-dinitrobenzene
mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5
0.45
1-chloro-2,4-dinitrobenzene
mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5
0.46
1-chloro-2,4-dinitrobenzene
-
pH and temperature not specified in the publication
0.46
1-chloro-2,4-dinitrobenzene
-
recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication
0.46
1-chloro-2,4-dinitrobenzene
mutant enzyme V54A, at pH 8.0 and 30°C
0.47
1-chloro-2,4-dinitrobenzene
pH 6.5, 30°C, recombinant GSTU4-4 mutant S13A
0.52
1-chloro-2,4-dinitrobenzene
mutant enzyme R214A, in 0.1 M phosphate buffer pH 6.5
0.53
1-chloro-2,4-dinitrobenzene
wild type enzyme, in 0.1 M phosphate buffer pH 6.5
0.557
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 35°C, in presence of cloquintocet-mexyl
0.572
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 35°C, in presence of fluxofenim
0.59
1-chloro-2,4-dinitrobenzene
isoform GSTrho, at pH 6.5 and 25°C
0.602
1-chloro-2,4-dinitrobenzene
wild type enzyme, pH and temperature not specified in the publication
0.629
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 35°C, in presence of fenchlorazole-ethyl
0.63
1-chloro-2,4-dinitrobenzene
isoform GSTmu, at pH 6.5 and 25°C
0.64
1-chloro-2,4-dinitrobenzene
mutant enzyme D94N, in phosphate buffer (pH 6.5), at 25°C
0.64
1-chloro-2,4-dinitrobenzene
mutant enzyme Y215H, in 0.1 M phosphate buffer pH 6.5
0.654
1-chloro-2,4-dinitrobenzene
recombinant mutant S22A, pH 6.5, temperature not specified in the publication
0.66
1-chloro-2,4-dinitrobenzene
His-tagged recombinant wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
0.72
1-chloro-2,4-dinitrobenzene
-
pH 7.4, 30°C, purified recombinant isozyme P1-1
0.753
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 35°C, in presence of oxabetrinil
0.84
1-chloro-2,4-dinitrobenzene
wild type enzyme, at pH 8.0 and 30°C
0.88
1-chloro-2,4-dinitrobenzene
mutant enzyme Y215E, in 0.1 M phosphate buffer pH 6.5
0.88
1-chloro-2,4-dinitrobenzene
untagged recombinant wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
0.9
1-chloro-2,4-dinitrobenzene
mutant enzyme R74Q, in phosphate buffer (pH 6.5), at 25°C
0.93
1-chloro-2,4-dinitrobenzene
mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5
0.93
1-chloro-2,4-dinitrobenzene
mutant enzyme R68A, at pH 8.0 and 30°C
0.97
1-chloro-2,4-dinitrobenzene
mutant enzyme C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
1
1-chloro-2,4-dinitrobenzene
wild type enzyme, in phosphate buffer (pH 6.5), at 25°C
1
1-chloro-2,4-dinitrobenzene
mutant enzyme H52A, at pH 8.0 and 30°C
1
1-chloro-2,4-dinitrobenzene
mutant enzyme S67A, at pH 8.0 and 30°C
1.003
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU3, at pH 6.5 and 25°C
1.01
1-chloro-2,4-dinitrobenzene
mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5
1.11
1-chloro-2,4-dinitrobenzene
mutant enzyme R70Q, in phosphate buffer (pH 6.5) ,at 25°C
1.2
1-chloro-2,4-dinitrobenzene
wild type enzyme, in 0.1 M phosphate buffer at pH 6.5
1.222
1-chloro-2,4-dinitrobenzene
isoform GSTO7, at pH 6.5 and 20°C
1.26
1-chloro-2,4-dinitrobenzene
mutant enzyme T67A, in phosphate buffer (pH 6.5), at 25°C
1.273
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU13, at pH 6.5 and 25°C
1.3
1-chloro-2,4-dinitrobenzene
mutant enzyme H106A, at 25°C, in 100 mM phosphate buffer (pH 6.5)
1.372
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 35°C, in presence of fenchlorazole-ethyl
1.4
1-chloro-2,4-dinitrobenzene
mutant enzyme A113V, in 0.1 M phosphate buffer at pH 6.5
1.5
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 37°C, recombinant AtuGSTH1-1
1.59
1-chloro-2,4-dinitrobenzene
-
pH and temperature not specified in the publication
1.62
1-chloro-2,4-dinitrobenzene
mutant enzyme D90N, in phosphate buffer (pH 6.5), at 25°C
1.66
1-chloro-2,4-dinitrobenzene
mutant enzyme C14A/C47A/C101A/C169A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
1.69
1-chloro-2,4-dinitrobenzene
isoform GSTU16, pH not specified in the publication, at 30°C
1.7
1-chloro-2,4-dinitrobenzene
mutant enzyme E66A, at pH 8.0 and 30°C
1.77
1-chloro-2,4-dinitrobenzene
mutant enzyme F212Y, in 0.1 M phosphate buffer pH 6.5
1.78
1-chloro-2,4-dinitrobenzene
mutant enzyme F212W, in 0.1 M phosphate buffer pH 6.5
1.82
1-chloro-2,4-dinitrobenzene
-
isoform GTT1.2, at pH 7.5, temperature not specified in the publication
1.9
1-chloro-2,4-dinitrobenzene
wild type enzyme, at 25°C, in 100 mM phosphate buffer (pH 6.5)
1.9
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 37°C, recombinant AtuGSTH1-1 mutant S25A
1.92
1-chloro-2,4-dinitrobenzene
mutant enzyme F216A, in 0.1 M phosphate buffer pH 6.5
2.074
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU7, at pH 6.5 and 25°C
2.1
1-chloro-2,4-dinitrobenzene
mutant enzyme H106F, at 25°C, in 100 mM phosphate buffer (pH 6.5)
2.144
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU12, at pH 6.5 and 25°C
2.266
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU6, at pH 6.5 and 25°C
2.284
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 25°C, recombinant catalytically active monoclonal antibody fragment ScFv2F3
2.3
1-chloro-2,4-dinitrobenzene
mutant enzyme I104V/A113V, in 0.1 M phosphate buffer at pH 6.5
2.35
1-chloro-2,4-dinitrobenzene
mutant enzyme F212H, in 0.1 M phosphate buffer pH 6.5
2.364
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU5, at pH 6.5 and 25°C
2.37
1-chloro-2,4-dinitrobenzene
mutant enzyme T174A, in 0.1 M phosphate buffer pH 6.5
2.4
1-chloro-2,4-dinitrobenzene
mutant enzyme C14A/C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
2.628
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU8, at pH 6.5 and 25°C
2.785
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU9, at pH 6.5 and 25°C
2.9
1-chloro-2,4-dinitrobenzene
mutant enzyme I104V, in 0.1 M phosphate buffer at pH 6.5
3.06
1-chloro-2,4-dinitrobenzene
mutant enzyme F212A, in 0.1 M phosphate buffer pH 6.5
3.2
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 37°C, recombinant AtuGSTH1-1 mutant F22A
3.372
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU11, at pH 6.5 and 25°C
3.46
1-chloro-2,4-dinitrobenzene
-
in phosphate buffer (0.1 M, pH 6.5), at 25°C
3.69
1-chloro-2,4-dinitrobenzene
mutant enzyme F212E, in 0.1 M phosphate buffer pH 6.5
4.269
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU14, at pH 6.5 and 25°C
4.3
1-chloro-2,4-dinitrobenzene
mutant enzyme C10A, at 25°C, in 100 mM phosphate buffer (pH 6.5)
4.4
1-chloro-2,4-dinitrobenzene
mutant enzyme C10S, at 25°C, in 100 mM phosphate buffer (pH 6.5)
4.5
1-chloro-2,4-dinitrobenzene
-
at pH 7.0, temperature not specified in the publication
4.819
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU2, at pH 6.5 and 25°C
5.01
1-chloro-2,4-dinitrobenzene
-
isoform Ure2pB1, at pH 7.5, temperature not specified in the publication
5.121
1-chloro-2,4-dinitrobenzene
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU4, at pH 6.5 and 25°C
6.4
1-chloro-2,4-dinitrobenzene
-
pH 7.5, 37°C, enzyme of Nanyang population
7.1
1-chloro-2,4-dinitrobenzene
-
pH 6.5, 37°C, recombinant AtuGSTH1-1 mutant R187A
7.2
1-chloro-2,4-dinitrobenzene
-
Ure2p mutant A122C, at 25°C and pH 8.0, in the presence of 1 mM dithiothreitol
10.07
1-chloro-2,4-dinitrobenzene
-
in phosphate buffer (0.1 M, pH 6.5), at 25°C
29.2
1-chloro-2,4-dinitrobenzene
-
Ure2p mutant N124V, at 25°C and pH 8.0
113
1-chloro-2,4-dinitrobenzene
-
recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication
223.6
1-chloro-2,4-dinitrobenzene
-
pH 7.5, 37°C, enzyme of Hezhou population
0.324
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
wild type enzyme, at 25°C, pH not specified in the publication
0.47 - 1
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
mutant enzyme Y113A, at 25°C, pH not specified in the publication
0.781
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
mutant enzyme N109A, at 25°C, pH not specified in the publication
0.882
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
mutant enzyme F116A, at 25°C, pH not specified in the publication
1.117
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
mutant enzyme W161A, at 25°C, pH not specified in the publication
1.487
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
mutant enzyme L112A, at 25°C, pH not specified in the publication
3.661
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
mutant enzyme M17A, at 25°C, pH not specified in the publication
3.866
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
mutant enzyme F162A, at 25°C, pH not specified in the publication
0.00003
4-methylumbelliferyl acetate
mutant enzyme N24A, pH and temperature not specified in the publication
0.00094
4-methylumbelliferyl acetate
wild type enzyme, pH and temperature not specified in the publication
773.44
4-nitrobenzyl chloride
mutant enzyme Y12C, pH and temperature not specified in the publication
1727
4-nitrobenzyl chloride
wild type enzyme, pH and temperature not specified in the publication
0.191
4-nitrophenyl butyrate
recombinant mutant S22A, pH 6.5, temperature not specified in the publication
0.774
4-nitrophenyl butyrate
recombinant wild-type, pH 6.5, temperature not specified in the publication
0.00022
5-chloromethylfluorescein diacetate
mutant enzyme N24A, pH and temperature not specified in the publication
0.00033
5-chloromethylfluorescein diacetate
mutant enzyme N22A, pH and temperature not specified in the publication
0.00049
5-chloromethylfluorescein diacetate
mutant enzyme Y46A, pH and temperature not specified in the publication
0.01162
5-chloromethylfluorescein diacetate
wild type enzyme, pH and temperature not specified in the publication
0.002
7-chloro-4-nitrobenz-2-oxa-1,3-diazole
mutant enzyme I104V/A113V, in 0.1 M phosphate buffer at pH 6.5
0.004
7-chloro-4-nitrobenz-2-oxa-1,3-diazole
wild type enzyme, in 0.1 M phosphate buffer at pH 6.5
0.007
7-chloro-4-nitrobenz-2-oxa-1,3-diazole
mutant enzyme I104V, in 0.1 M phosphate buffer at pH 6.5
0.011
7-chloro-4-nitrobenz-2-oxa-1,3-diazole
mutant enzyme A113V, in 0.1 M phosphate buffer at pH 6.5
0.324
allyl-isothiocyanate
isoform GSTU45, pH not specified in the publication, at 30°C
0.759
allyl-isothiocyanate
isoform GSTU16, pH not specified in the publication, at 30°C
0.02
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone
Q2G4B4; Q2G4B5
wild type enzyme, at pH 8.0 and 30°C
0.022
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone
Q2G4B4; Q2G4B5
mutant enzyme S14A, at pH 8.0 and 30°C
0.1
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone
Q2G4B4; Q2G4B5
mutant enzyme S15A, at pH 8.0 and 30°C
0.12
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone
Q2G4B4; Q2G4B5
mutant enzyme S14A/S15A, at pH 8.0 and 30°C
0.25
beta-(2-methoxyphenoxy)-gamma-hydroxypropiovanillone
Q2G4B4; Q2G4B5
mutant enzyme N14A, at pH 8.0 and 30°C
0.038
cumene hydroperoxide
isoform GSTU16, pH not specified in the publication, at 30°C
0.11
cumene hydroperoxide
wild type enzyme, pH and temperature not specified in the publication
4.853
cumene hydroperoxide
isoform GSTU45, pH not specified in the publication, at 30°C
5.3 - 6
cumene hydroperoxide
mutant enzyme N24A, pH and temperature not specified in the publication
14.3
cumene hydroperoxide
-
at pH 7.0, temperature not specified in the publication
0.39
dehydroascorbate
isoform GstO2B, at pH 7.6 and 30°C
1.03
dehydroascorbate
isoform GstO2A, at pH 7.6 and 30°C
1.66
dehydroascorbate
isoform GSTO4, at pH 6.5 and 20°C
0.01
Ethacrynic acid
mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5
0.032
Ethacrynic acid
mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5
0.037
Ethacrynic acid
mutant enzyme Q112A, in 0.1 M phosphate buffer pH 6.5
0.06
Ethacrynic acid
mutant enzyme104V, in 0.1 M phosphate buffer at pH 6.5
0.065
Ethacrynic acid
wild type enzyme, in 0.1 M phosphate buffer pH 6.5
0.08
Ethacrynic acid
mutant enzyme104V, in 0.1 M phosphate buffer at pH 6.5
0.09
Ethacrynic acid
mutant enzyme104V, in 0.1 M phosphate buffer at pH 6.5
0.1
Ethacrynic acid
mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5
0.21
Ethacrynic acid
wild type enzyme, in 0.1 M phosphate buffer at pH 6.5
0.003
glutathione
mutant enzyme I104V/A113V, in the presence of cosubstrate 7-chloro-4-nitrobenz-2-oxa-1,3-diazole, in 0.1 M phosphate buffer at pH 6.5
0.007
glutathione
mutant enzyme I104V, in the presence of cosubstrate 7-chloro-4-nitrobenz-2-oxa-1,3-diazole, in 0.1 M phosphate buffer at pH 6.5
0.008
glutathione
wild type enzyme, in the presence of cosubstrate 7-chloro-4-nitrobenz-2-oxa-1,3-diazole, in 0.1 M phosphate buffer at pH 6.5
0.014
glutathione
mutant enzyme A113V, in the presence of cosubstrate 7-chloro-4-nitrobenz-2-oxa-1,3-diazole, in 0.1 M phosphate buffer at pH 6.5
0.0214
glutathione
-
with cumene hydroperoxide as cosubstrate, pH and temperature not specified in the publication
0.0286
glutathione
with cumene hydroperoxide as cosubstrate, at pH 6.5 and 25°C
0.047
glutathione
Thermosynechococcus vestitus
at 30°C in 0.1 M potassium phosphate buffer
0.05
glutathione
mutant enzyme C10S, at 25°C, in 100 mM phosphate buffer (pH 6.5)
0.0524
glutathione
-
with fluorodifen as cosubstrate, pH and temperature not specified in the publication
0.058
glutathione
wild type enzyme, at 25°C, pH not specified in the publication
0.0615
glutathione
-
with tert-butyl peroxide as cosubstrate, pH and temperature not specified in the publication
0.0679
glutathione
with 1-chloro-2,4-dinitrobenzene as cosubstrate, at pH 6.5 and 25°C
0.0771
glutathione
-
with 1-chloro-2,4-dinitrobenzene as cosubstrate, pH and temperature not specified in the publication
0.08
glutathione
mutant enzyme I104V/A113V, in the presence of cosubstrate 1-chloro-2,4-dinitrobenzene, in 0.1 M phosphate buffer at pH 6.5
0.085
glutathione
-
pH 6.5, 37°C, recombinant AtuGSTH1-1 mutant F22A, with 1-chloro-2,4-dinitrobenzene
0.087
glutathione
with tert-butyl peroxide as cosubstrate, at pH 6.5 and 25°C
0.11
glutathione
-
pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
0.11
glutathione
mutant enzyme I104V, in the presence of cosubstrate 1-chloro-2,4-dinitrobenzene, in 0.1 M phosphate buffer at pH 6.5
0.12
glutathione
mutant enzyme Y113A, at 25°C, pH not specified in the publication
0.12
glutathione
-
with cumene hydroperoxide as cosubstrate, at pH 7.0, temperature not specified in the publication
0.13
glutathione
mutant enzyme A113V, in the presence of cosubstrate 1-chloro-2,4-dinitrobenzene, in 0.1 M phosphate buffer at pH 6.5
0.14
glutathione
-
pH 6.5, 37°C, recombinant AtuGSTH1-1 mutant S25A, with 1-chloro-2,4-dinitrobenzene
0.14
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU3, at pH 6.5 and 25°C
0.141
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU6, at pH 6.5 and 25°C
0.146
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU4, at pH 6.5 and 25°C
0.15
glutathione
wild type enzyme, in the presence of cosubstrate 1-chloro-2,4-dinitrobenzene, in 0.1 M phosphate buffer at pH 6.5
0.166
glutathione
mutant enzyme F116A, at 25°C, pH not specified in the publication
0.17
glutathione
mutant enzyme I104V, in the presence of cosubstrate ethacrynic acid, in 0.1 M phosphate buffer at pH 6.5
0.17
glutathione
wild type enzyme, in the presence of cosubstrate ethacrynic acid, in 0.1 M phosphate buffer at pH 6.5
0.192
glutathione
mutant enzyme N109A, at 25°C, pH not specified in the publication
0.2
glutathione
mutant enzyme I104V/A113V, in the presence of cosubstrate ethacrynic acid, in 0.1 M phosphate buffer at pH 6.5
0.208
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU9, at pH 6.5 and 25°C
0.21
glutathione
mutant enzyme A113V, in the presence of cosubstrate ethacrynic acid, in 0.1 M phosphate buffer at pH 6.5
0.216
glutathione
recombinant wild-type, pH 6.5, temperature not specified in the publication
0.221
glutathione
mutant enzyme W161A, at 25°C, pH not specified in the publication
0.23
glutathione
-
GSTD1, pH and temperature not specified in the publication
0.24
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU5, at pH 6.5 and 25°C
0.25
glutathione
mutant enzyme T67A, in phosphate buffer (pH 6.5), at 25°C
0.25
glutathione
-
with phenethyl isothiocyanate as cosubstrate, at pH 7.0, temperature not specified in the publication
0.262
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU13, at pH 6.5 and 25°C
0.284
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU10, at pH 6.5 and 25°C
0.3
glutathione
wild type enzyme, at 25°C, in 100 mM phosphate buffer (pH 6.5)
0.3
glutathione
-
pH 6.5, 37°C, recombinant AtuGSTH1-1, with 1-chloro-2,4-dinitrobenzene
0.307
glutathione
mutant enzyme L112A, at 25°C, pH not specified in the publication
0.32
glutathione
untagged recombinant wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
0.33
glutathione
mutant enzyme T174A, in 0.1 M phosphate buffer pH 6.5
0.333
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU12, at pH 6.5 and 25°C
0.34
glutathione
-
pH 6.5, 37°C, recombinant AtuGSTH1-1 mutant R187A, with 1-chloro-2,4-dinitrobenzene
0.36
glutathione
mutant enzyme F162A, at 25°C, pH not specified in the publication
0.37
glutathione
-
pH 6.5, 25°C, recombinant enzyme, with (2E,4Z)-5-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid
0.37
glutathione
His-tagged recombinant wild type enzyme, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
0.38
glutathione
mutant enzyme F212E, in 0.1 M phosphate buffer pH 6.5
0.39
glutathione
-
pH 6.5, 25°C, recombinant enzyme, with (2Z,4E)-3-chloro-6-(3,5-dichlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid
0.45
glutathione
-
isoform GSTO1, at pH 7.5, temperature not specified in the publication
0.45
glutathione
-
with 1-chloro-2,4-dinitrobenzene as cosubstrate, at pH 7.0, temperature not specified in the publication
0.455
glutathione
-
pH 6.5, 25°C, recombinant catalytically active monoclonal antibody fragment ScFv2F3
0.473
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU1, at pH 6.5 and 25°C
0.48
glutathione
mutant enzyme F212Y, in 0.1 M phosphate buffer pH 6.5
0.491
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU14, at pH 6.5 and 25°C
0.5
glutathione
-
isoform GSTFuA1, at pH 7.5, temperature not specified in the publication
0.53
glutathione
mutant enzyme C14A/C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
0.53
glutathione
mutant enzyme F212H, in 0.1 M phosphate buffer pH 6.5
0.615
glutathione
recombinant mutant S22A, pH 6.5, temperature not specified in the publication
0.62
glutathione
mutant enzyme R214A, in 0.1 M phosphate buffer pH 6.5
0.65
glutathione
mutant enzyme F212A, in 0.1 M phosphate buffer pH 6.5
0.67
glutathione
mutant enzyme F212W, in 0.1 M phosphate buffer pH 6.5
0.7
glutathione
-
Ure2p mutant A122C, at 25°C and pH 8.0, in the presence of 1 mM dithiothreitol
0.73
glutathione
mutant enzyme D90N, in phosphate buffer (pH 6.5), at 25°C
0.73
glutathione
mutant enzyme Y215A, in 0.1 M phosphate buffer pH 6.5
0.732
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU11, at pH 6.5 and 25°C
0.743
glutathione
mutant enzyme M17A, at 25°C, pH not specified in the publication
0.76
glutathione
mutant enzyme Y215E, in 0.1 M phosphate buffer pH 6.5
0.78
glutathione
mutant enzyme Y215S, in 0.1 M phosphate buffer pH 6.5
0.78
glutathione
isoform GSTrho, at pH 6.5 and 25°C
0.85
glutathione
mutant enzyme F216A, in 0.1 M phosphate buffer pH 6.5
0.85
glutathione
mutant enzyme Y215F, in 0.1 M phosphate buffer pH 6.5
0.87
glutathione
-
isoform Ure2pB1, at pH 7.5, temperature not specified in the publication
0.88
glutathione
mutant enzyme R74Q, in phosphate buffer (pH 6.5), at 25°C
0.893
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU8, at pH 6.5 and 25°C
0.99
glutathione
mutant enzyme Y215H, in 0.1 M phosphate buffer pH 6.5
0.996
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU2, at pH 6.5 and 25°C
1.03
glutathione
mutant enzyme C14A/C47A/C101A/C169A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
1.183
glutathione
A0A4Y5QZW9, A0A4Y5QZX0, A0A4Y5QZX2, A0A4Y5QZX3, A0A4Y5QZX9, A0A4Y5QZZ8, A0A4Y5R032, A0A4Y5R087, A0A4Y5R0B2, A0A4Y5R0C1, A0A4Y5R0K8, A0A4Y5R0L5, A0A4Y5R2M7, A0A4Y5R2N2
isoform GSTU7, at pH 6.5 and 25°C
1.33
glutathione
-
isoform GTT1.2, at pH 7.5, temperature not specified in the publication
1.4
glutathione
mutant enzyme H106A, at 25°C, in 100 mM phosphate buffer (pH 6.5)
1.56
glutathione
mutant enzyme C47A/C101A, at 30°C, in 0.1 M potassium phosphate buffer, pH 6.5
1.7
glutathione
-
pH 6.5, 37°C, recombinant AtuGSTH1-1, with 2-hydroxyethyl disulfide
1.9
glutathione
mutant enzyme H106F, at 25°C, in 100 mM phosphate buffer (pH 6.5)
2.03
glutathione
mutant enzyme F212L, in 0.1 M phosphate buffer pH 6.5
2.2
glutathione
-
recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication
2.5
glutathione
mutant enzyme C10A, at 25°C, in 100 mM phosphate buffer (pH 6.5)
3
glutathione
-
recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication
3.8
glutathione
-
recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication
4.1
glutathione
mutant enzyme R70Q, in phosphate buffer (pH 6.5), at 25°C
4.1
glutathione
-
recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication
4.21
glutathione
isoform GSTmu, at pH 6.5 and 25°C
7
glutathione
-
recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication
13.2
glutathione
-
recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication
13.6
glutathione
-
recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication
26.1
glutathione
-
recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication
466.1
glutathione
mutant enzyme Y12C, pH and temperature not specified in the publication
532.43
glutathione
wild type enzyme, pH and temperature not specified in the publication
0.0139
GSH
-
pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid
0.0146
GSH
-
pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid
0.0178
GSH
-
pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid
0.025
GSH
-
pH 7.4, 37°C, glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid
0.047
GSH
-
pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c, reaction with maleylacetone
0.064
GSH
-
pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone
0.117
GSH
-
pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone
0.134
GSH
-
pH 7.4, 37°C, glutathione transferase Zeta 1c-1c, reaction with maleylacetone
0.287
GSH
-
pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c, reaction with maleylacetone
0.298
GSH
-
pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c, reaction with (+)-2-bromo-3-(4-nitrophenyl)propanoic acid
0.4
GSH
-
30°C, mutant enzyme Y108A, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane
0.4
GSH
-
30°C, wild-type enzyme, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane
0.46
GSH
-
30°C, mutant enzyme Y108F, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane
0.48
GSH
-
30°C, mutant enzyme Y108W, reaction with 1,2-epoxy-3-(p-nitrophenoxy)propane
0.88
GSH
-
30°C, pH 6.5, wild-type enzyme, reaction with DELTA5-androstene-3,17-dione
0.064
maleylacetone
-
pH 7.4, 37°C, mutant enzyme S15A of glutathione transferase Zeta 1c-1c
0.068
maleylacetone
-
pH 7.4, 37°C, mutant enzyme C16A of glutathione transferase Zeta 1c-1c
0.223
maleylacetone
-
pH 7.4, 37°C, mutant enzyme R175K of glutathione transferase Zeta 1c-1c
0.768
maleylacetone
-
pH 7.4, 37°C, mutant enzyme R175A of glutathione transferase Zeta 1c-1c
0.0065
phenethyl isothiocyanate
isoform GSTU16, pH not specified in the publication, at 30°C
0.036
phenethyl isothiocyanate
isoform GSTU45, pH not specified in the publication, at 30°C
0.047
phenethyl isothiocyanate
-
at pH 7.0, temperature not specified in the publication
0.119
phenethyl isothiocyanate
recombinant wild-type, pH 6.5, temperature not specified in the publication
0.14
phenethyl isothiocyanate
isoform GSTO7, at pH 6.5 and 20°C
0.16
phenethyl-isothiocyanate
Thermosynechococcus vestitus
at 30°C in 0.1 M potassium phosphate buffer
0.75
phenethyl-isothiocyanate
-
at 30°C in 0.1 M potassium phosphate buffer
0.0256
sulforaphane
isoform GSTU45, pH not specified in the publication, at 30°C
6.178
sulforaphane
isoform GSTU16, pH not specified in the publication, at 30°C
additional information
additional information
-
a sequential steady-state kinetic mechanism
-
additional information
additional information
-
biphasic kinetics of enzyme from healthy and muscle with experimental trichinellosis in presence or absence of albendazole, overview
-
additional information
additional information
-
Cu2+ effects on Km, overview
-
additional information
additional information
-
kinetics of formation of specific mono(glutathionyl) or bis(glutathionyl) conjugates
-
additional information
additional information
kinetics of glutathione peroxidase activity, EC 1.11.1.9, overview
-
additional information
additional information
-
kinetics of glutathione peroxidase activity, EC 1.11.1.9, overview
-
additional information
additional information
-
kinetics, single-enzyme Michaelis-Menten model
-
additional information
additional information
-
kinetics, single-enzyme Michaelis-Menten model
-
additional information
additional information
-
kinetics, single-enzyme Michaelis-Menten model
-
additional information
additional information
-
kinetics, single-enzyme Michaelis-Menten model
-
additional information
additional information
-
kinetics, single-enzyme MichaelisMenten model
-
additional information
additional information
quantitative screening for improved catalytic activity of mutant isozymes GST T1-1 towards CMAC using flow cytometry, up to a 20000fold increase in kcat/KM compared to wild-type GSTT1-1 is achieved, overview
-
additional information
additional information
-
quantitative screening for improved catalytic activity of mutant isozymes GST T1-1 towards CMAC using flow cytometry, up to a 20000fold increase in kcat/KM compared to wild-type GSTT1-1 is achieved, overview
-
additional information
additional information
-
steady-state kinetics of the recombinant catalytically active monoclonal antibody fragment ScFv2F3, dependence on substrate concentrations, overview
-
additional information
additional information
-
steady-state kinetics using 1-chloro-2,4-dinitrobenzene as substrate
-
additional information
additional information
-
stopped flow rapid kinetics, overview
-
additional information
additional information
-
the enzyme does not show Michaelis-Menten kinetics
-
additional information
additional information
-
thermodynamics and kinetics of recombinant wild-type and mutant enzymes, the rate-limiting step of the reaction is viscosity-dependent product release
-
additional information
additional information
-
steady-state and ligand binding kinetics, recombinant enzyme
-
additional information
additional information
-
kinetic analysis, overview
-
additional information
additional information
-
kinetic analysis of wild-type and mutant enzymes, overview
-
additional information
additional information
kinetic analysis of wild-type and mutant enzymes, overview
-
additional information
additional information
-
kinetic analysis of wild-type and mutant enzymes, overview
-
additional information
additional information
-
kinetic analysis, overview. GSTM2-2 does not follow a rapid equilibrium Michaelis-Menten mechanism. However, the low catalytic activity of the GSTM2-2 isoenzyme and its high affinity for brostallicin prevent an accurate kinetic study
-
additional information
additional information
-
Michaelis-Menten steady-state kinetic studies, recombinant cytosolic isozyme, overview
-
additional information
additional information
positive cooperativity for GSH and 1-chloro-2,4-dinitrobenzene, respectively, steady-state kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
purified enzyme, Michaelis-Menten kinetic analysis, overview
-
additional information
additional information
-
single turnover stopped flow kinetics and equilibrium dialysis analysis of GSH biniding affinity and stoichiometry, dissociation constants, overview
-
additional information
additional information
-
steady-state kinetics of recombinant His6-tagged isozyme GST T1-1, substrate 1,3-bis-(2-chloroethyl)-1-nitrosourea
-
additional information
additional information
-
steady-state kinetics of recombinant His6-tagged wild-type and mutant isozymes GST T1-1, substrate 1,3-bis-(2-chloroethyl)-1-nitrosourea
-
additional information
additional information
thermodynamic and molecular docking of wild-type and R15L mutant enzymes, overview
-
additional information
additional information
-
thermodynamic and molecular docking of wild-type and R15L mutant enzymes, overview
-
additional information
additional information
-
Michaelis-Menten steady-state kinetics
-
additional information
additional information
GST5118 kinetic mechanism, overview
-
additional information
additional information
-
GST5118 kinetic mechanism, overview
-
additional information
additional information
-
isothermal titration calorimetry reveals high-affinity binding for GSTF2 and GSTF3, which is enhanced in the presence of glutathione and by the other heterocyclic ligands, and allosteric enhancement in glutathione-conjugating activity
-
additional information
additional information
-
pseudo-first order kinetic constants, simplified Michaelis-Menten steady-state model for simulation of the overall dependence of the rate of product formationon either substrate or enzyme concentrations, with substrate 1-chloro-2,4-dinitrobenzene
-
additional information
additional information
-
recombinant hGSTk, steady-state kinetics, overview
-
additional information
additional information
steady-state kinetic analysis of wild-type and mutannt isozymes GSTU4-4, overview
-
additional information
additional information
-
steady-state kinetic analysis of wild-type and mutannt isozymes GSTU4-4, overview
-
additional information
additional information
-
steady-state Michaelis-Menten kinetic analysis of AtuGSTH1-1
-
additional information
additional information
the native enzyme and the C169A mutant exhibit MichaelisMenten kinetics, but all other thiol to alanine mutants exhibit sigmoidal kinetics to varying degrees, ping-pong kinetics of mutant C169A, overview
-
additional information
additional information
-
the native enzyme and the C169A mutant exhibit MichaelisMenten kinetics, but all other thiol to alanine mutants exhibit sigmoidal kinetics to varying degrees, ping-pong kinetics of mutant C169A, overview
-
