Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.5.1.20: rubber cis-polyprenylcistransferase

This is an abbreviated version!
For detailed information about rubber cis-polyprenylcistransferase, go to the full flat file.

Word Map on EC 2.5.1.20

Reaction

poly-cis-polyprenyl diphosphate
+
isopentenyl diphosphate
=
diphosphate
 +
a poly-cis-polyprenyl diphosphate longer by one C5 unit

Synonyms

allyltransferase, rubber, chain-elongating enzyme cis-prenyltransferase, cis prenyl transferase, cis-1,4-polyprenylcistransferase, cis-prenyl transferase, cis-prenyltransferase, cisprenyltransferase, CPT, CPT3, CPT6, HRT1, HRT2, isopentenyl pyrophosphate cis-1,4-polyisoprenyl transferase, laticifer-specific rubber-forming enzyme, More, RT-ase complex, rTase, rubber allyltransferase, rubber polymerase, rubber prenyltransferase, rubber transferase, rubber transferase 1, rubber transferase 2, rubber transferase complex, RubCPT1, TkCPT1, TkCPT2, TkCPT3, TkCPTL1

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.20 rubber cis-polyprenylcistransferase

Purification

Purification on EC 2.5.1.20 - rubber cis-polyprenylcistransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DE52, Sephacryl S-200, hexyl-Sepharose, Mono Q
-
ammonium sulfate, Sephadex G-25, DEAE-cellulose, partial purification
-
centrifugation to separate small rubber particles from the lower layer, dilution with 0.01 M phosphate buffer, pH 7.0 to 30% dry rubber content, washed particles are produced by washing with 0.25 M Tris-HCl, pH 8.0, with 1% Tween-20 and 5 mM 2-mercaptoethanol, applied to a Ultrogel AcA-44 column, centrifuged and diluted to 15% dry rubber content, highly purified small particles are produced by further centrifugation with 0.5% Triton X-100, exchange by buffer and centrifugation, cream phase diluted to 15% dry rubber content
-
Ni-NTA resin column chromatography
Parthenium argentatum makes rubber in parenchyma cells, which must be homogenized to release the rubber particles, the method for purification of enzymatically active rubber particles from the plant requires homogenization that also releases copious amounts of proteases and other degradative enzymes, with a resultant decrease in enzyme stability and half-life, overview
-
pH 5.4, ammonium sulfate, Sephadex G-100, DEAE-Sephadex, partial purification
-
purification of rubber particles containing the enzyme by harvesting latex from petioles of roots, separating latex fractions by centrifugation with extraction buffer (100 mM Tris-HCl, pH 7.8, 350 mM sorbitol, 10 mM NaCl, 5 mM MgCl2, 5 mM DTT), rubber phase is further centrifuged, washed with buffer and used for enzyme assays
-