2.5.1.22: spermine synthase
This is an abbreviated version!
For detailed information about spermine synthase, go to the full flat file.
Word Map on EC 2.5.1.22
-
2.5.1.22
-
polyamine
-
putrescine
-
s-adenosylmethionine
-
ornithine
-
aminopropyltransferases
-
snyder-robinson
-
alpha-difluoromethylornithine
-
acaulis5
-
thermospermine
-
trans-4-methylcyclohexylamine
-
gyro
-
kyphoscoliosis
-
agriculture
-
adometdc
-
2.5.1.16
-
medicine
- 2.5.1.22
- polyamine
- putrescine
- s-adenosylmethionine
- ornithine
- aminopropyltransferases
-
snyder-robinson
- alpha-difluoromethylornithine
- acaulis5
- thermospermine
- trans-4-methylcyclohexylamine
-
gyro
-
kyphoscoliosis
- agriculture
- adometdc
-
2.5.1.16
- medicine
Reaction
Synonyms
ACL5, aminopropyltransferase, spermidine, OsSPMS1, SMS, spermidine aminopropyltransferase, spermine synthase, spermine synthase 1, spermine synthetase, Spm synthase, SpmS, SPMS1, SpmSyn, synthase, spermine
ECTree
Advanced search results
Cloned
Cloned on EC 2.5.1.22 - spermine synthase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
DNA fragment encoding human enzyme is amplified by PCR and subcloned into the pET28a-LIC vector downstream of the polyhistidine coding region. Enzyme is expressed in the Escherichia coli BL21-Codon Plus(DE3)-RIL strain by the addition of 1 mM isopropyl1-thio-beta-D-galactopyranoside. The N-terminally truncated SpmSyn mutants are generated by PCR and subcloned into the pET28a-LIC vector.
DNA sequence determination and analysis of gene SPMS, construction of expression vectors for recombinant expression in transgenic plants/mutants of genes ACAULIS5, i.e. ACL5, and SPMS
-
enzyme overexpression in transgenic mice under control of a composite CMV-IE enhancer-chicken beta-actin promotor, 4 separate founder CAG/SpmS mice are analysed: enzyme expression in all tissues, mostly highly increased compared to the wild-type mice, overview
-
expressed in a yeast mutant deficient in Spm synthase, and in the Arabidopsis dwarf mutant acl5
-
expression of human spermine synthase in CAGSMS line 8 mice from a composite CMV-IE enhancer/chicken beta-actin promoter. Transgenic expression of spermine synthase in the Gy mice reverse all of the increases in dcAdoMet content and AdoMetDC
-