2.5.1.3: thiamine phosphate synthase

This is an abbreviated version!
For detailed information about thiamine phosphate synthase, go to the full flat file.

Reaction

Synonyms

2-methyl-4-amino-5-hydroxymethylpyrimidinepyrophosphate:4-methyl-5-(2'-phosphoethyl)-thiazole 2-methyl-4-aminopyrimidine-5-methenyltransferase, hydroxyethylthiazole kinase/thiamine-phosphate pyrophosphorylase, PF0544, pyrophosphorylase, thiamin phosphate, SSO2532, TH16, THI6, thiamin monophosphate pyrophosphorylase, thiamin phosphate diphosphorylase, thiamin phosphate pyrophosphorylase, thiamin phosphate synthase, thiamin-phosphate pyrophosphorylase, thiamine monophosphate pyrophosphorylase, thiamine phosphate pyrophosphorylase, thiamine phosphate synthase, thiamine-phosphate diphosphorylase, thiamine-phosphate pyrophosphorylase, thiamine-phosphate synthase, thiaminephosphate pyrophosphorylase, ThiE, ThiL, ThiN protein, TM0723, TM1872, TMP pyrophosphorylase, TMP-PPase, TMPPase, TMPS, TPS, YjbQ

ECTree

     2 Transferases

         2.5 Transferring alkyl or aryl groups, other than methyl groups

             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

                2.5.1.3 thiamine phosphate synthase

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Results	in table
35289	AA Sequence
1	CAS Registry Number
12	Cloned(Commentary)
2	Crystallization (Commentary)
2	General Information
32	Inhibitors
2	Ki Value [mM]
10	KM Value [mM]
1	Localization
22	Metals/Ions
10	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
36	Organism
11	Pathway
52	PDB ID
5	pH Optimum
3	pH Range
1	pI Value
12	Protein Variants
8	Purification (Commentary)
4	Reaction
1	Reaction Type
32	Reference
7	Source Tissue
9	Specific Activity [micromol/min/mg]
4	Storage Stability
43	Substrates and Products (Substrate)
7	Subunits
60	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Range [°C]
5	Temperature Stability [°C]
1	Turnover Number [1/s]

KM Value

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KM Value on EC 2.5.1.3 - thiamine phosphate synthase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0004 - 0.007

2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate

2 entries

0.0007 - 0.001

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate

3 entries

0.0128

4-amino-5-hydroxymethyl-2-methylpyrimidine diphosphate

Zea mays

Q2UVH9

37°C, pH 8.0

671903

0.0085

4-methyl-5-(2-hydroxyethyl)thiazole phosphate

Zea mays

Q2UVH9

-

671903

0.0012

4-methyl-5-beta-hydroxyethylthiazole phosphate

Escherichia coli

-

wild type enzyme, in buffer containing 50 mM phosphate and 10 mM MgSO4, at pH 8.0 and 37°C

722984

0.0004

2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate

Escherichia coli

-

-

639722, 639723

0.007

2-(4-methyl-1,3-thiazol-5-yl)ethyl phosphate

Saccharomyces cerevisiae

-

pH 9.2, 37°C

639721

0.0007

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate

Escherichia coli

-

wild type enzyme, in buffer containing 50 mM phosphate and 10 mM MgSO4, at pH 8.0 and 37°C

722984

0.00085

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate

Escherichia coli

-

-

639722, 639723

0.001

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate

Saccharomyces cerevisiae

-

pH 9.2, 37°C

639721