2.5.1.30: heptaprenyl diphosphate synthase
This is an abbreviated version!
For detailed information about heptaprenyl diphosphate synthase, go to the full flat file.
Word Map on EC 2.5.1.30
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2.5.1.30
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prenyltransferase
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allylic
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synthases
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menaquinone-7
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stearothermophilus
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geranylgeranyl
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hexaprenyl
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medium-chain
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micrococcus
-
luteus
-
heteromeric
-
hepes
-
mops
-
zwitterionic
-
undecaprenyl
-
pharmacology
- 2.5.1.30
- prenyltransferase
-
allylic
- synthases
- menaquinone-7
- stearothermophilus
-
geranylgeranyl
-
hexaprenyl
-
medium-chain
-
micrococcus
- luteus
-
heteromeric
- hepes
- mops
-
zwitterionic
- undecaprenyl
- pharmacology
Reaction
+ 4 isopentenyl diphosphate = 4 diphosphate +
Synonyms
HepPP synthase, HepPPS, HepPS, heptaprenyl pyrophosphate synthetase, heptaprenylpyrophosphate synthetase
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Purification
Purification on EC 2.5.1.30 - heptaprenyl diphosphate synthase
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the two subunits (subunit I and subunit II) of the HepPP synthase are overproduced in Escherichia coli cells respectively and purified. These subunits are modified with six histidines (polyhistidine) at the N-terminal
to explore the dynamic interaction of the two dissociable components during catalysis, expression vector systems for the two structural genes, gerC1 and gerC3, are constructed separately, and the two components are overproduced in Escherichia coli cells. Each component is purified homogeneously