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2.5.1.30: heptaprenyl diphosphate synthase

This is an abbreviated version!
For detailed information about heptaprenyl diphosphate synthase, go to the full flat file.

Word Map on EC 2.5.1.30

Reaction

(2E,6E)-farnesyl diphosphate
+ 4 isopentenyl diphosphate = 4 diphosphate +
all-trans-heptaprenyl diphosphate

Synonyms

HepPP synthase, HepPPS, HepPS, heptaprenyl pyrophosphate synthetase, heptaprenylpyrophosphate synthetase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.30 heptaprenyl diphosphate synthase

Purification

Purification on EC 2.5.1.30 - heptaprenyl diphosphate synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
separation of component I and component II
the two subunits (subunit I and subunit II) of the HepPP synthase are overproduced in Escherichia coli cells respectively and purified. These subunits are modified with six histidines (polyhistidine) at the N-terminal
to explore the dynamic interaction of the two dissociable components during catalysis, expression vector systems for the two structural genes, gerC1 and gerC3, are constructed separately, and the two components are overproduced in Escherichia coli cells. Each component is purified homogeneously