Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.5.1.31: ditrans,polycis-undecaprenyl-diphosphate synthase [(2E,6E)-farnesyl-diphosphate specific]

This is an abbreviated version!
For detailed information about ditrans,polycis-undecaprenyl-diphosphate synthase [(2E,6E)-farnesyl-diphosphate specific], go to the full flat file.

Word Map on EC 2.5.1.31

Reaction

(2E,6E)-farnesyl diphosphate
+ 8 isopentenyl diphosphate = 8 diphosphate +
ditrans,octacis-undecaprenyl diphosphate

Synonyms

bactoprenyl-diphosphate synthase, C55-OO synthetase, C55PP synthetase, cis,polyprenyl diphosphate synthase, cis-prenyl chain elongating enzyme, cis-type undecaprenyl pyrophosphate synthase, CPDS, DDPPs, dehydrodolichyl diphosphate synthase, di-trans,poly-cis-decaprenylcistransferase, di-trans,poly-cis-undecaprenyl-diphosphate synthase, di-trans-poly-cis-decaprenyl cistransferase, isosesquilavandulyl diphosphate synthase, Mcl22, More, synthetase, undecaprenyl pyrophosphate, undecaprenyl diphosphate phosphatase, undecaprenyl diphosphate synthase, undecaprenyl diphosphate synthetase, undecaprenyl pyrophosphate synthase, undecaprenyl pyrophosphate synthetase, undecaprenyl-diphosphate synthase, undecaprenyl-pyrophosphate synthase, UPP synthase, UPP synthetase, UPP, C55 synthase, UPPs, UPS, Z-prenyl diphosphate synthase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.31 ditrans,polycis-undecaprenyl-diphosphate synthase [(2E,6E)-farnesyl-diphosphate specific]

Cloned

Cloned on EC 2.5.1.31 - ditrans,polycis-undecaprenyl-diphosphate synthase [(2E,6E)-farnesyl-diphosphate specific]

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli, no sequence similarity between E-prenyl diphosphate and Z-prenyl diphosphate synthases
expression in Escherichia coli. Thioredoxin and His tag are utilized to increase the solubility of the recombinant protein and facilitate its purification using Ni-nitrilotriacetic acid column
-
expression of mutant enzmyes W31F, W75F, W91F, W149F, W207F, and W221F as His-tag fusion proteins using Escherichia coli BL21 as host cells
-
expression of mutant enzymes R197S, R203S and E216Q in Escherichia coli
gene uppS, expression of GFP-tagged UPPS in CHO cell endoplasmic reticulum, expression in Saccharomyces cerevisiae microsomes, ability of the Escherichia coli undecaprenyl pyrophosphate synthase to complement the loss of the yeast cis isoprenyltransferase in the rer2DELTA mutant
-
gene upps, recombinant expression of His-tagged enzyme in Escherichia coli strain C41
gene uppS, recombinant His-tagged enzyme expression in Escherichia coli strain BL21
gene uppS, recombinant N-terminally His-tagged enzyme expression in Escherichia coli strain BL21(DE3)
gene uppS1 containing a single-point mutation in the ribosome-binding site of the uppS gene, resulting in mutant strain HB13564, genotyping, DNA and amino acid sequence determination and analysis
-
mutated enzymes are overproduced in Escherichia coli
overexpression of His-tagged wild-type and mutant enzymes
-
overexpression of His-tagged wild-type and mutant enzymes in strain BL21(DE3)
-
overexpression of wild-type and mutant enzymes in Escherichia coli strain JM109