2.5.1.48: cystathionine gamma-synthase
This is an abbreviated version!
For detailed information about cystathionine gamma-synthase, go to the full flat file.
Word Map on EC 2.5.1.48
-
2.5.1.48
-
homocystinuria
-
transsulfuration
-
beta-lyase
-
gamma-lyase
-
sulfhydrylase
-
o-acetylhomoserine
-
gamma-replacement
-
l-cystathionine
-
cystathionase
-
o-phosphohomoserine
-
paucicostata
-
gamma-elimination
-
homocystine
-
s-methylmethionine
-
remethylation
-
synthesis
- 2.5.1.48
- homocystinuria
-
transsulfuration
-
beta-lyase
-
gamma-lyase
-
sulfhydrylase
- o-acetylhomoserine
-
gamma-replacement
- l-cystathionine
- cystathionase
- o-phosphohomoserine
- paucicostata
-
gamma-elimination
- homocystine
- s-methylmethionine
-
remethylation
- synthesis
Reaction
Synonyms
AtCGS, CGS, CGS1, CS,26, cystathionine gamma-synthase, cystathionine synthase, cystathionine synthetase, cystathionine-gamma-synthase, EC 4.2.99.9, homoserine O-transsuccinylase, homoserine transsuccinylase, HTS, MetB, O-succinyl-L-homoserine succinate-lyase (adding cysteine), O-succinylhomoserine (Thiol)-lyase, O-succinylhomoserine synthase, O-succinylhomoserine synthetase, synthase, cystathionine gamma-
ECTree
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Reference
Reference on EC 2.5.1.48 - cystathionine gamma-synthase
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Yamagata, S.; D'Andrea, R.J.; Fujisaki, S.; Isaji, M.; Nakamura, K.
Cloning and bacterial expression of the CYS3 gene encoding cystathionine gamma-lyase of Saccharomyces cerevisiae and the physicochemical and enzymatic properties of the protein
J. Bacteriol.
175
4800-4808
1993
Saccharomyces cerevisiae
Martel, A.; Bouthier de la Tour, C.; le Goffic, F.
Pyridoxal 5 phosphate binding site of Escherichia coli beta cystathionase and cystathionine gamma synthase comparison of their sequences
Biochem. Biophys. Res. Commun.
147
565-571
1987
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium
Giovanelli, J.; Mudd, S.H.; Datko, A.H.; Thompson, G.A.
Effects of orthophosphate and adenosine 5'-phosphate on threonine synthase and cystathionine gamma-synthase of Lemna paucicostata Hegelm. 6746
Plant Physiol.
81
577-583
1986
Lemna aequinoctialis
Nagasawa, T.; Kanzaki, H.; Yamada, H.
Cystathionine gamma-lyase of Streptomyces phaeochromogenes. The occurrence of cystathionine gamma-lyase in filamentous bacteria and its purification and characterization
J. Biol. Chem.
259
10393-10403
1984
Streptomyces phaeochromogenes
Wiebers, J.L.; Garner, H.R.
Acyl derivatives of homoserine as substrates for homocysteine synthesis in Neurospora crassa, yeast and Escherichia coli
J. Biol. Chem.
242
5644-5649
1967
Escherichia coli
Flavin, M.; Slaughter, C.
Enzymatic synthesis of homocysteine or methionine directly from O-succinyl-homoserine
Biochim. Biophys. Acta
132
400-405
1967
Salmonella enterica subsp. enterica serovar Typhimurium
Guggenheim, S.; Flavin, M.
Proton retention in the gamma-elimination reaction catalysed by cystathionine gamma-synthase
Biochim. Biophys. Acta
151
664-669
1968
Salmonella enterica subsp. enterica serovar Typhimurium
Kerr, D.; Flavin, M.
Inhibition of cystathionine gamma-synthase by S-adenosylmethionine: A control mechanism for methionine synthesis in Neurospora
Biochim. Biophys. Acta
177
177-179
1969
Neurospora crassa
Guggenheim, S.; Flavin, M.
Cystathionine gamma-synthase from Salmonella. Beta elimination and replacement reactions and inhibition by O-succinylserine
J. Biol. Chem.
244
3722-3727
1969
Salmonella enterica subsp. enterica serovar Typhimurium
Guggenheim, S.; Flavin, M.
Cystathionine gamma-synthase. A pyridoxal phosphate enzyme catalyzing rapid exchanges of beta and alpha hydrogen atoms in amino acids
J. Biol. Chem.
244
6217-6227
1969
Salmonella enterica subsp. enterica serovar Typhimurium
Kaplan, M.; Guggenheim, S.
Cystathionine gamma-synthase (Salmonella)
Methods Enzymol.
17B
425-433
1971
Salmonella enterica subsp. enterica serovar Typhimurium
-
Posner B.I.
Cystathionine-synthase: studies on the replacement reaction
Biochim. Biophys. Acta
276
277-283
1972
Salmonella enterica subsp. enterica serovar Typhimurium
Posner, B.I.; Flavin, M.
Cystathionine -synthase. Studies of hydrogen exchange reactions
J. Biol. Chem.
247
6402-6411
1972
Salmonella enterica subsp. enterica serovar Typhimurium
Posner, B.I.; Flavin, M.
Cystathionine -synthase. The nature of intramolecular proton shifts in the elimination reaction
J. Biol. Chem.
247
6412-6419
1972
Salmonella enterica subsp. enterica serovar Typhimurium
Marcotte,P.; Walsh, C.
Active site-directed inactivation of cystathionine gamma-synthetase and glutamic pyruvic transaminase by propargylglycine
Biochem. Biophys. Res. Commun.
62
677-682
1975
Salmonella enterica subsp. enterica serovar Typhimurium
Thompson, G.A.; Datko, A.H.; Mudd, S.H.
Methionine synthesis in Lemna. Inhibition of cystathionine gamma-synthase by propargylglycine
Plant Physiol.
70
1347-1352
1982
Lemna aequinoctialis
Tran, S.V.; Schaeffer, E.; Bertrand, O.; Mariuzza, R.; Ferrara, P.
Appendix. Purification, molecular weight, and NH2-terminal sequence of cystathionine gamma-synthase of Escherichia coli
J. Biol. Chem.
258
14872-14873
1983
Escherichia coli
Kanzaki, H.; Kobayashi, M.; Nagasawa, T.; Yamada, H.
Distribution to two kinds of cystathionine gamma-synthase in various bacteria
FEMS Microbiol. Lett.
33
65-68
1987
Acinetobacter calcoaceticus, Agrobacterium tumefaciens, Alcaligenes faecalis, Aneurinibacillus aneurinilyticus, Bacillus pumilus, Bacillus subtilis, Escherichia coli, Lysinibacillus sphaericus, Pectobacterium carotovorum, Pseudomonas dacunhae, Pseudomonas putida, Salmonella enterica subsp. enterica serovar Typhimurium
-
Kanzaki, H.; Kobayashi, M.; Nagasawa, T.; Yamada, H.
Purification and characterization of cystathionine gamma-synthase type II from Bacillus sphaericus
Eur. J. Biochem.
163
105-112
1987
Lysinibacillus sphaericus
Kanzaki, H.; Kobayashi, M.; Nagasawa, T.; Yamada, H.
Production of L-cystathionine using bacterial cystathionine gamma-synthase
Appl. Microbiol. Biotechnol.
25
322-326
1987
Lysinibacillus sphaericus, Pectobacterium carotovorum
-
Dawson, J.C.; Anderson, J.W.
Comparative enzymology of cystathionine and selenocystathionine synthesis of selenium-accumulator and non-accumulator plants
Phytochemistry
28
51-55
1989
Astragalus bisulcatus, Astragalus hamosus, Astragalus racemosus, Astragalus sinicus, Neptunia amplexicaulis
-
Brzovic, P.; Litzenberger Holbrook, E; Greene, R.C.; Dunn, M.F.
Reaction mechanism of Escherichia coli cystathionine gamma-synthase: direct evidence for a pyridoxamine derivative of vinylglyoxylate as a key intermediate in pyridoxal phosphate dependent gamma-elimination and gamma-replacement reactions
Biochemistry
29
442-451
1990
Salmonella enterica subsp. enterica serovar Typhimurium
Litzenberger Holbrook, E.; Greene, R.C.; Heilig Krueger, J.
Purification and properties of cystathionine gamma-synthase from overproducing strains of Escherichia coli
Biochemistry
29
435-442
1990
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium
Homer, R.J.; Kim, M.S.; LeMaster, D.M.
The use of cystathionine gamma-synthase in the production of alpha and chiral beta deuterated amino acids
Anal. Biochem.
215
211-215
1993
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium
Ono, B.I.; Ishi, N.; Naito, K.; Miyoshi, S.I.; Shinoda, S.; Yamamoto, S.; Ohmori, S.
Cystathionine gamma-lyase of Saccharomyces cerevisiae: structural gene and cystathionine gamma-synthase activity
Yeast
9
389-397
1993
Saccharomyces cerevisiae
Kreft, B.D.; Townsend, A.; Pohlenz, H.D.; Laber, B.
Purification and properties of cystathionine gamma-synthase from wheat (Triticum aestivum L.)
Plant Physiol.
104
1215-1220
1994
Triticum aestivum
Ravanel, S.; Droux, M.; Douce, R.
Methionine biosynthesis in higher plants. I. Purification and characterization of cystathionine gamma-synthase from spinach chloroplasts
Arch. Biochem. Biophys.
316
572-584
1995
Spinacia oleracea
Clausen, T.; Huber, R.; Prade, L.; Wahl, M.C.; Messerschmidt, A.
Crystal structure of Escherichia coli cystathionine gamma-synthase at 1.5 A resolution
EMBO J.
17
6827-6838
1998
Escherichia coli (P00935), Escherichia coli
Ravanel, S.; Gakiere, B.; Job, D.; Douce, R.
Cystathionine gamma-synthase from Arabidopsis thaliana: purification and biochemical characterization of the recombinant enzyme overexpressed in Escherichia coli
Biochem. J.
331
639-648
1998
Arabidopsis thaliana
-
Ominato, K.; Akita, H.; Suzuki, A.; Kijima, F.; Yoshino, T.; Yoshino, M.; Chiba, Y.; Onouchi, H.; Naito, S.
Identification of a short highly conserved amino acid sequence as the functional region required for posttranscriptional autoregulation of the cystathionine gamma-synthase gene in Arabidopsis
J. Biol. Chem.
277
36380-36386
2002
Solanum tuberosum
Hwang, B.J.; Kim, Y.; Kim, H.B.; Hwang, H.J.; Kim, J.H.; Lee, H.S.
Analysis of Corynebacterium glutamicum methionine biosynthetic pathway: isolation and analysis of metB encoding cystathionine gamma-synthase: Isolation and analysis of metB encoding cystathionine gamma synthase
Mol. Cells
9
300-308
1999
Corynebacterium glutamicum
Clausen, T.; Wahl, M.C.; Messerschmidt, A.; Huber, R.; Fuhrmann, J.C.; Laber, B.; Streber, W.; Steegborn, C.
Cloning, purification, and characterization of cystathionine g-synthase from Nicotiana tabacum
Biol. Chem.
380
1237-1242
1999
Nicotiana tabacum
Kim, J.; Lee, M.; Chalam, R.; Martin, M.N.; Leustek, T.; Boerjan, W.
Constitutive overexpression of cystathionine gamma-synthase in Arabidopsis leads to accumulation of soluble methionine and S-methylmethionine
Plant Physiol.
128
95-107
2002
Arabidopsis thaliana (P55217)
Hacham, Y.; Avraham, T.; Amir, R.
The N-terminal region of Arabidopsis cystathionine gamma-synthase plays an important regulatory role in methionine metabolism
Plant Physiol.
128
454-462
2002
Arabidopsis thaliana
Steegborn, C.; Messerschmidt, A.; Laber, B.; Streber, W.; Huber, R.; Clausen, T.
The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity
J. Mol. Biol.
290
983-996
1999
Nicotiana tabacum
Kreft, O.; Hoefgen, R.; Hesse, H.
Functional analysis of cystathionine gamma-synthase in genetically engineered potato plants
Plant Physiol.
131
1843-1854
2003
Solanum tuberosum (Q9MT29), Solanum tuberosum
Aitken, S.M.; Kim, D.H.; Kirsch, J.F.
Escherichia coli cystathionine gamma-synthase does not obey ping-pong kinetics. Novel continuous assays for the elimination and substitution reactions
Biochemistry
42
11297-11306
2003
Escherichia coli
Van Huysen, T.; Abdel-Ghany, S.; Hale, K.L.; LeDuc, D.; Terry, N.; Pilon-Smits, E.A.
Overexpression of cystathionine-gamma-synthase enhances selenium volatilization in Brassica juncea
Planta
218
71-78
2003
Arabidopsis thaliana
Chiba, Y.; Sakurai, R.; Yoshino, M.; Ominato, K.; Ishikawa, M.; Onouchi, H.; Naito, S.
S-adenosyl-L-methionine is an effector in the posttranscriptional autoregulation of the cystathionine gamma-synthase gene in Arabidopsis
Proc. Natl. Acad. Sci. USA
100
10225-10230
2003
Arabidopsis thaliana (P55217), Arabidopsis thaliana
Avraham, T.; Amir, R.
The expression level of threonine synthase and cystathionine-gamma-synthase is influenced by the level of both threonine and methionine in Arabidopsis plants
Transgenic Res.
14
299-311
2005
Arabidopsis thaliana
Ziegler, K.; Noble, S.M.; Mutumanje, E.; Bishop, B.; Huddler, D.P.; Born, T.L.
Identification of catalytic cysteine, histidine, and lysine residues in Escherichia coli homoserine transsuccinylase
Biochemistry
46
2674-2683
2007
Escherichia coli
Goudarzi, M.; Born, T.L.
Purification and characterization of Thermotoga maritima homoserine transsuccinylase indicates it is a transacetylase
Extremophiles
10
469-478
2006
Escherichia coli
Avraham, T.; Badani, H.; Galili, S.; Amir, R.
Enhanced levels of methionine and cysteine in transgenic alfalfa (Medicago sativa L.) plants over-expressing the Arabidopsis cystathionine gamma-synthase gene
Plant Biotechnol. J.
3
71-79
2005
Arabidopsis thaliana
Hacham, Y.; Schuster, G.; Amir, R.
An in vivo internal deletion in the N-terminus region of Arabidopsis cystathionine gamma-synthase results in CGS expression that is insensitive to methionine
Plant J.
45
955-967
2006
Arabidopsis thaliana
Katz, Y.S.; Galili, G.; Amir, R.
Regulatory role of cystathionine-gamma-synthase and de novo synthesis of methionine in ethylene production during tomato fruit ripening
Plant Mol. Biol.
61
255-268
2006
Solanum lycopersicum
Kong, Y.; Wu, D.; Bai, H.; Han, C.; Chen, J.; Chen, L.; Hu, L.; Jiang, H.; Shen, X.
Enzymatic characterization and inhibitor discovery of a new cystathionine gamma-synthase from Helicobacter pylori
J. Biochem.
143
59-68
2008
Helicobacter pylori (Q1M0P5), Helicobacter pylori, Helicobacter pylori SS (Q1M0P5)
Hacham, Y.; Matityahu, I.; Schuster, G.; Amir, R.
Overexpression of mutated forms of aspartate kinase and cystathionine gamma-synthase in tobacco leaves resulted in the high accumulation of methionine and threonine
Plant J.
54
260-271
2008
Arabidopsis thaliana
Loizeau, K.; Gambonnet, B.; Zhang, G.F.; Curien, G.; Jabrin, S.; Van Der Straeten, D.; Lambert, W.E.; Rebeille, F.; Ravanel, S.
Regulation of one-carbon metabolism in Arabidopsis: the N-terminal regulatory domain of cystathionine gamma-synthase is cleaved in response to folate starvation
Plant Physiol.
145
491-503
2007
Arabidopsis thaliana
Farsi, A.; Lodha, P.H.; Skanes, J.E.; Los, H.; Kalidindi, N.; Aitken, S.M.
Interconversion of a pair of active-site residues in Escherichia coli cystathionine gamma-synthase, E. coli cystathionine beta-lyase, and Saccharomyces cerevisiae cystathionine gamma-lyase and development of tools for the investigation of their mechanisms
Biochem. Cell Biol.
87
445-457
2009
Escherichia coli
Saha, B.; Mukherjee, S.; Das, A.K.
Molecular characterization of Mycobacterium tuberculosis cystathionine gamma synthase--apo- and holoforms
Int. J. Biol. Macromol.
44
385-392
2009
Mycobacterium tuberculosis
Clifton, M.C.; Abendroth, J.; Edwards, T.E.; Leibly, D.J.; Gillespie, A.K.; Ferrell, M.; Dieterich, S.H.; Exley, I.; Staker, B.L.; Myler, P.J.; Van Voorhis, W.C.; Stewart, L.J.
Structure of the cystathionine gamma-synthase MetB from Mycobacterium ulcerans
Acta Crystallogr. Sect. F
67
1154-1158
2011
Mycobacterium ulcerans (A0PKT3), Mycobacterium ulcerans Agy99 (A0PKT3)
Ngo, H.P.; Kim, J.K.; Kim, S.H.; Pham, T.V.; Tran, T.H.; Nguyen, D.D.; Kim, J.G.; Chung, S.; Ahn, Y.J.; Kang, L.W.
Expression, crystallization and preliminary X-ray crystallographic analysis of cystathionine gamma-synthase (XometB) from Xanthomonas oryzae pv. oryzae
Acta Crystallogr. Sect. F
68
1515-1517
2012
Xanthomonas oryzae
Jaworski, A.F.; Lodha, P.H.; Manders, A.L.; Aitken, S.M.
Exploration of the active site of Escherichia coli cystathionine gamma-synthase
Protein Sci.
21
1662-1671
2012
Escherichia coli
Morneau, D.J.; Jaworski, A.F.; Aitken, S.M.
Identification of cystathionine gamma-synthase and threonine synthase from Cicer arietinum and Lens culinaris
Biochem. Cell Biol.
91
95-101
2013
Cicer arietinum (L0EK12), Lens culinaris (L0EKR2)
Huang, J.F.; Zhang, B.; Shen, Z.Y.; Liu, Z.Q.; Zheng, Y.G.
Metabolic engineering of E. coli for the production of O-succinyl-L-homoserine with high yield
3 Biotech
8
310
2018
Escherichia coli (P00935), Escherichia coli K12 (P00935)
Sato, D.; Shiba, T.; Mizuno, S.; Kawamura, A.; Hanada, S.; Yamada, T.; Shinozaki, M.; Yanagitani, M.; Tamura, T.; Inagaki, K.; Harada, S.
The hyperthermophilic cystathionine gamma-synthase from the aerobic crenarchaeon Sulfolobus tokodaii expression, purification, crystallization and structural insights
Acta Crystallogr. Sect. F
73
152-158
2017
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
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