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2.5.1.56: N-acetylneuraminate synthase

This is an abbreviated version!
For detailed information about N-acetylneuraminate synthase, go to the full flat file.

Word Map on EC 2.5.1.56

Reaction

phosphoenolpyruvate
+
N-acetyl-D-mannosamine
+
H2O
=
phosphate
+
N-acetylneuraminate

Synonyms

(NANA) condensing enzyme, EC 4.1.3.19, N-acetylneuraminate lyase synthase, N-acetylneuraminate pyruvate-lyase (pyruvate-phosphorylating), N-acetylneuraminic acid condensing enzyme, N-acetylneuraminic acid synthase, NANA condensing enzyme, NANA synthase, NANAS, NANS, NeuAc synthase, NeuB, NeuB1, NeuNAc synthase, NmeNANAS, sialic acid synthase, synthase, N-acetylneuraminate

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.56 N-acetylneuraminate synthase

Purification

Purification on EC 2.5.1.56 - N-acetylneuraminate synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
cell harvesting by centrifugation, resuspension, lysing in high-pressure homogenizer, centrifugation, chromatographic procedures including ion-exchange and gel filtration steps, concentration
expression of the native neuB gene product enzyme in E. coli results in a product that is prone to proteolysis during purification so the protein is tagged with a hexa-histidine tag at its N-terminus and rapidly purified
-
Ni2+ affinity column chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2 by ultrafiltration, nickel affinity chromatography, and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) pLysS by nickel affinity chromatography and gel filtration