2.5.1.59: protein geranylgeranyltransferase type I

This is an abbreviated version!
For detailed information about protein geranylgeranyltransferase type I, go to the full flat file.

Reaction

Synonyms

CAAX geranylgeranyltransferase, CaaX prenyltransferase, CaGGTase-I, CDC43, geranylgeranyl protein transferase type I, geranylgeranyltransferase I, geranylgeranyltransferase type I, geranylgeranyltransferase type-I, geranylgeranyltransferase-1, geranylgeranyltransferase-I, geranylgeranyltransferaseI, GGT, GGT I, GGTalpha, GGTase, GGTase I, Ggtase-1, GGTase-I, GGTaseI, GGTbeta, GGTI, mammalian GGTase-I, PGGT, PGGT beta, PGGT I, PGGT-I, PGGTase-I, PGGTaseI, Ppggb, protein geranylgeranyltransferase, protein geranylgeranyltransferase I, protein geranylgeranyltransferase type I, protein geranylgeranyltransferase type-I, protein geranylgeranyltransferase-I, type I protein geranylgeranyltransferase

ECTree

     2 Transferases

         2.5 Transferring alkyl or aryl groups, other than methyl groups

             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

                2.5.1.59 protein geranylgeranyltransferase type I

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Results	in table
2	Activating Compound
1401	AA Sequence
7	Application
1	CAS Registry Number
19	Cloned(Commentary)
5	Crystallization (Commentary)
346	Disease/ Diagnostics
32	General Information
41	IC50 Value
117	Inhibitors
4	kcat/KM [mM/s]
14	Ki Value [mM]
28	KM Value [mM]
12	Localization
30	Metals/Ions
14	Molecular Weight [Da]
57	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
73	Organism
150	PDB ID
3	pH Optimum
1	pH Range
1	pH Stability
1	Posttranslational Modification
15	Protein Variants
15	Purification (Commentary)
17	Reaction
73	Reference
58	Source Tissue
3	Specific Activity [micromol/min/mg]
162	Substrates and Products (Substrate)
18	Subunits
105	Synonyms
1	Systematic Name
32	Turnover Number [1/s]

Metals Ions

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Metals Ions on EC 2.5.1.59 - protein geranylgeranyltransferase type I

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Cd2+

5 entries

Mg2+

7 entries

Mn2+

Bos taurus

-

60% of maximal activity in the presence of 0.5 mM alone

636539

Zinc

Rattus norvegicus

-

all of the GGTase-I complexes contain a zinc ion bound at full occupancy with a B-factor comparable with the surrounding protein residues. The zinc ion is coordinated by three residues, Asp269b, Cys271b and His321b

658547

Zn2+

11 entries

additional information

6 entries

Cd2+

Bos taurus

-

the zinc in enzyme can be replaced by Cd2+

636539

Cd2+

Bos taurus

-

80% of maximal activity in the presence of 0.5 mM alone

636539

Cd2+

Rattus norvegicus

-

the zinc in enzyme can be replaced by Cd2+

636541

Cd2+

Rattus norvegicus

-

Cd-substituted enzyme has altered specificities with regard to utilization of both peptide and isoprenoid substrates

636541

Cd2+

Saccharomyces cerevisiae

-

the zinc in enzyme can be replaced by Cd2+

636537

Mg2+

Bos taurus

-

20% of maximal activity in the presence of 0.5 mM alone

636539

Mg2+

Candida albicans

Q9Y765

for maximum reaction rate

687774

Mg2+

Rattus norvegicus

-

no requirement

636541

Mg2+

Rattus norvegicus

-

prenylation rate constant of wild type enzyme is not dependent on Mg2+ and is about 20fold slower than the maximal rate constant catalyzed by protein farnesyltransferase. Prenylation rate constant in mutant Kbeta311A or Kbeta311D decreases in absence of magnesium 9–41fold without significantly affecting the binding affinityof either substrate. Furthermore, the geranylgeranylation rate constant is enhanced by the addition of Mg2+ for Kbeta311A and Kbeta311D GGTase I 2–5-fold compared with wild type GGTase I. These results demonstrate that lysine beta311 of GGTase I partially replaces the catalytic function of Mg2+ observed in protein farnesyltransferase

659363

Mg2+

Saccharomyces cerevisiae

-

no requirement

636537

Mg2+

Saccharomyces cerevisiae

-

requirement, optimal activity at 0.5 mM in presence of 0.1 mM Zn2+

636543

Mg2+

Saccharomyces cerevisiae

-

required, optimal activity of EDTA-treated enzyme at 0.5 mM, Mg2+ probably activates the diphosphate leaving group of enzyme, and is essential for transferring the bound peptide substrate

636545

Zn2+

Bos taurus

-

enzyme contains tightly bound Zn2+ which is required for its catalytic activity

636545

Zn2+

Bos taurus

-

required, but no activity in presence of Zn2+ alone, only when either 0.1 mM Mg2+, Mn2+ or Cd2+ is additionally present

636539

Zn2+

Candida albicans

Q9Y765

activating the cysteine thiolate of the substrate

687774

Zn2+

Escherichia coli

-

catalytic zinc ion coordinates the thiol of the peptide substrate

671997

Zn2+

Homo sapiens

P49354, P53609

required for the enzymatic activities of GGTase-I, bound at the beta-subunit

738097

Zn2+

Rattus norvegicus

-

A zinc metalloenzyme. The Zn2+ is required for peptide, but not for isoprenoid, substrate binding

636541

Zn2+

Rattus norvegicus

-

enzyme contains a single zinc atom

636540

Zn2+

Rattus norvegicus

-

required for catalysis and peptide binding, one zinc ion per protein dimer

675272

Zn2+

Rattus norvegicus

P53610, Q04631

required for the enzymatic activities of GGTase-I, bound at the beta-subunit, coordinated by residues Aspbeta269, Cysbeta271 and Hisbeta321 in GGTase-I

738097

Zn2+

Saccharomyces cerevisiae

-

A zinc metalloenzyme. The Zn2+ is required for peptide, but not for isoprenoid, substrate binding

636537

Zn2+

Saccharomyces cerevisiae

-

requirement, optimal activity of EDTA-treated enzyme at 0.01 mM in presence of 1 mM Mg2+

636543, 636545

additional information

Bos taurus

-

not: Co2+

636539

additional information

Bos taurus

-

metals not needed for complex formation between enzyme and geranylgeranyl diphosphate, but addition of prenyl acceptor only results in prenyl transfer in presence of Mg2+ and Zn2+

636539

additional information

Homo sapiens

P49354

Mg2+ is not required

738097

additional information

Homo sapiens

P53609

Mg2+ is not required

738097

additional information

Rattus norvegicus

P53610

Mg2+ is not required

738097

additional information

Rattus norvegicus

Q04631

Mg2+ is not required

738097