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2.5.1.60: protein geranylgeranyltransferase type II

This is an abbreviated version!
For detailed information about protein geranylgeranyltransferase type II, go to the full flat file.

Word Map on EC 2.5.1.60

Reaction

geranylgeranyl diphosphate
+
protein-cysteine
=
S-geranylgeranyl-protein
 +
diphosphate

Synonyms

AtRGTA1, AtRGTA2, AtRGTB1, Chm, geranylgeranyl transferase type I subunit alpha 1, geranylgeranyl transferase type I subunit alpha 2, geranylgeranyl transferase type II subunit beta 1, geranylgeranyl transferase type II subunit beta 2, geranylgeranyl transferase type-1 subunit alpha 1, geranylgeranyl transferase type-2 subunit alpha 2, geranylgeranyl transferase type-2 subunit beta 1, geranylgeranyl transferase type-2 subunit beta 2, geranylgeranyltransferase II, geranylgeranyltransferase type II, GGT2, GGTase-II, GGTase3, GGTaseII, PGGT-II, protein geranylgeranyltransferase type-II, PTAR1, Rab geranylgeranyl transferase, Rab geranylgeranyl transferase alpha subunit 2, Rab geranylgeranyl transferase beta subunit 1, Rab geranylgeranyl transferase beta subunit 2, Rab geranylgeranyl transferase lpha subunit 1, Rab geranylgeranyl-transferase, Rab geranylgeranyltransferase, Rab geranylgeranyltransferase type II, Rab GG transferase, Rab GGTase, Rab proteins geranylgeranyltransferase component A 1, RAB-GGT, Rab-GGT alpha 1, Rab-GGT alpha 2, Rab-GGT beta 1, Rab-GGT beta 2, Rab11a, RabGGT, RabGGTase, Rabggtb, REP/GGTase, RGGT, RGT alphabeta, RGTA1, RGTA2, RGTB1, RGTB2

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.60 protein geranylgeranyltransferase type II

Cloned

Cloned on EC 2.5.1.60 - protein geranylgeranyltransferase type II

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of the beta subunit of enzyme by means of the yeast two-hybrid system
development of a two plasmid expression system in Escherichia coli that achieves large quantities of enzyme
-
expressed in Escherichia coli
-
expressed in Sf9 cells
-
expression in Escherichia coli
-
expression in SF21 cells
-
expression in Sf9 cells with recombinant baculovirus
-
gene RGTA1, RGTA subunit sequence comparisons, recombinant expression of His-tagged and of c-Myc-tagged RGTA1 subunit in Saccharomyces cerevisiae strain YPH499
gene RGTA2, RGTA subunit sequence comparisons, recombinant expression of His-tagged and of c-Myc-tagged RGTA2 subunit in Saccharomyces cerevisiae strain YPH499. No detectable RGTA2-c-Myc is present in the pulldown eluate, indicating that RGTA2-c-Myc can only be pulled down in a complex with FLAG-tagged RGTB1/2
gene RGTB1, RGTA subunit sequence comparisons, recombinant expression of His-tagged and of FLAG-tagged RGTB1 subunit in Saccharomyces cerevisiae strain YPH499
gene rgtb1, the gene structure is very similar to that of its paralogue, AtRGTB2, except that the sequence corresponding to the first exon of AtRGTB2 is split into two shorter exons in the AtRGTB1 gene, sequnce comparison and expression analysis
gene rgtb2, located on the third chromosome, comprises nine exons, and its structure is very similar to that of its paralogue, AtRGTB1, except that the sequence corresponding to the first exon of AtRGTB2 is split into two shorter exons in the AtRGTB1 gene, sequnce comparison and expression analysis
recombinant enzyme produced in baculovirus and Escherichia coli system
-
recombinant enzyme produced in insect cells
-