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2.5.1.60: protein geranylgeranyltransferase type II

This is an abbreviated version!
For detailed information about protein geranylgeranyltransferase type II, go to the full flat file.

Word Map on EC 2.5.1.60

Reaction

geranylgeranyl diphosphate
+
protein-cysteine
=
S-geranylgeranyl-protein
+
diphosphate

Synonyms

AtRGTA1, AtRGTA2, AtRGTB1, Chm, geranylgeranyl transferase type I subunit alpha 1, geranylgeranyl transferase type I subunit alpha 2, geranylgeranyl transferase type II subunit beta 1, geranylgeranyl transferase type II subunit beta 2, geranylgeranyl transferase type-1 subunit alpha 1, geranylgeranyl transferase type-2 subunit alpha 2, geranylgeranyl transferase type-2 subunit beta 1, geranylgeranyl transferase type-2 subunit beta 2, geranylgeranyltransferase II, geranylgeranyltransferase type II, GGT2, GGTase-II, GGTase3, GGTaseII, PGGT-II, protein geranylgeranyltransferase type-II, PTAR1, Rab geranylgeranyl transferase, Rab geranylgeranyl transferase alpha subunit 2, Rab geranylgeranyl transferase beta subunit 1, Rab geranylgeranyl transferase beta subunit 2, Rab geranylgeranyl transferase lpha subunit 1, Rab geranylgeranyl-transferase, Rab geranylgeranyltransferase, Rab geranylgeranyltransferase type II, Rab GG transferase, Rab GGTase, Rab proteins geranylgeranyltransferase component A 1, RAB-GGT, Rab-GGT alpha 1, Rab-GGT alpha 2, Rab-GGT beta 1, Rab-GGT beta 2, Rab11a, RabGGT, RabGGTase, Rabggtb, REP/GGTase, RGGT, RGT alphabeta, RGTA1, RGTA2, RGTB1, RGTB2

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.60 protein geranylgeranyltransferase type II

Substrates Products

Substrates Products on EC 2.5.1.60 - protein geranylgeranyltransferase type II

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E,10Z)-3,7,11-trimethyl-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodeca-2,6,10-trien-1-yl diphosphate + Rab7 GTPase
(2E,6E,10Z)-3,7,11-trimethyl-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodeca-2,6,10-trien-1-yl-Rab7 GTPase + diphosphate
show the reaction diagram
-
in vitro prenylation assay with 4 microM Rab7 wild type or mutants, 6 mircoM Rab escort protein, 6 microM enzyme, 50 mM HEPES buffer, pH 7.2, containing 50 mM NaCl, 5 mM dithioerythritol, 2 mM MgCl2, and 10 mircoM GDP, 25°C, 50 microM of the geranylgeranyl diphosphate analogue (3,7,11-trimethyl-12-(7-nitro-benzo[1,2,5]oxadiazo-4-ylamino)-dodeca-2,6,10-triem-1) diphosphate, end-point assay with 6 microM Rab7 wild type or mutants, 10 microM Rab escort protein, 6 microM enzyme in the same buffer mixture, and 40 microM (3,7,11-trimethyl-12-(7-nitro-benzo[1,2,5]oxadiazo-4-ylamino)-dodeca-2,6,10-triem-1) diphosphate, 1.5 hours at 25°C
-
-
?
(3,7,11-trimethyl-12-(7-nitrobenzo[1,2,5]oxadiazo-4-ylamino)-dodeca-2,6,19-trien-1) diphosphate + Rab7
(3,7,11-trimethyl-12-(7-nitrobenzo[1,2,5]oxadiazo-4-ylamino)-dodeca-2,6,19-trien-1)-Rab7 + diphosphate
show the reaction diagram
-
geranylgeranyl diphosphate analogue and Rab7 as substrates in the presence of Rab escort protein, in vitro inhibition assays
-
-
?
biotinylated geranyl diphosphate + EYFP-Rab7
S-biotin-geranyl-Rab7 + diphosphate
show the reaction diagram
-
COS-7 cells are transfected with EYFP-Rab7 fusion protein and incubated with inhibitors, cells lysed after 24 h, clarified supernatant incubated in biotinylated geranyl diphosphate (geranylgeranyl diphosphate substrate analogue), recombinant RabGGTase and Rab escort protein, in vivo inhibitor studies
-
-
?
geranylgeranyl diphosphate + Arabidopsis thaliana RAB-ATa protein
S-geranylgeranyl-RAB GTPase + diphosphate
show the reaction diagram
-
homozygous insertion mutants (rgtb1-1 and rgtb1-2) of the major beta-subunit of Arabidopsis enzyme (RGTB1) lead to reduced enzyme activity (about 25%), 50 mM phosphate buffer, pH 7.6, 10 mM MgCl2, 5 mM DTT, 0.5 microM tritium-labelled all trans-geranylgeranyl pyrophosphate, 4 microg recombinant Arabidopsis thaliana RAB-A2a protein, 32°C, 40 min
-
-
?
geranylgeranyl diphosphate + FBXL2
geranylgeranyl-FBXL2 + diphosphate
show the reaction diagram
P53611; Q7Z6K3
FBXL2 is an ubiquitin-ligase
-
-
?
geranylgeranyl diphosphate + protein-cysteine
S-geranylgeranyl-protein + diphosphate
show the reaction diagram
geranylgeranyl diphosphate + Rab
S-geranylgeranyl-Rab + diphosphate
show the reaction diagram
-
His6- and gluthathione S-transferase-tagged Rab proteins such as canine Rab1a, human Rab27a, Rab5a, Rab18, Rab6a, Rab13, and mouse Rab23
-
-
?
geranylgeranyl diphosphate + Rab protein
S-geranylgeranyl-Rab protein + diphosphate
show the reaction diagram
geranylgeranyl diphosphate + Rab-protein-cysteine
S-geranylgeranyl-Rab-protein + diphosphate
show the reaction diagram
-
-
-
-
?
geranylgeranyl diphosphate + Rab1A
?
show the reaction diagram
-
substrate motif: -XXCC
-
-
?
geranylgeranyl diphosphate + Rab1B
?
show the reaction diagram
-
-
-
-
?
geranylgeranyl diphosphate + Rab3A
?
show the reaction diagram
-
substrate motif: -XCXC
-
-
?
geranylgeranyl diphosphate + Rab5A
?
show the reaction diagram
-
substrate motif: -CCXX
-
-
?
geranylgeranyl diphosphate + Rab7 protein
S-geranylgeranyl-Rab7 protein + diphosphate
show the reaction diagram
-
the N-terminal Cys207 is preferred to Cys205, activity with Rab7 mutants, overview
-
-
?
geranylgeranyl diphosphate + Rab8
?
show the reaction diagram
-
the Rab protein Rab8-GTPase, which end with a Cys-Val-Leu-Leu motif able to serve as a substrate for either geranylgeranyl transferase I and II, but modified predominantly by either geranylgeranyl transferase II in vitro, but the mutation of Y78D prevents Rab8 from serving as a substrate
-
-
?
geranylgeranyl diphosphate + Ypt1p
?
show the reaction diagram
[3,7,11-trimethyl-12-(7-nitrobenzo[1,2,5]oxadiazo-4-ylamino)dodeca-2,6,10-trien-1] diphosphate + Rab7 protein
?
show the reaction diagram
-
i.e. NBD-FPP, a fluorescent analogue of geranylgeranyl diphosphate, which is a lipid donor for RabGGTase
product is mono-NBD-farnesylated Rab7:REP-1 complex
-
?
additional information
?
-