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2.5.1.61: hydroxymethylbilane synthase

This is an abbreviated version!
For detailed information about hydroxymethylbilane synthase, go to the full flat file.

Word Map on EC 2.5.1.61

Reaction

4 porphobilinogen +

H2O
=
Hydroxymethylbilane
 + 4 NH3

Synonyms

(HMB)-synthase, AN0121.3, EC 4.3.1.8, HemC, hepatic porphobilinogen deaminase, HMB synthase, HMB-S, HMBS, HMBS1a-synthase, HMBS1b-synthase, HMBS2a-synthase, HMBS2b-synthase, human non-erythropoietic PBGD isoform, human PBGD, human porphobilinogen deaminase, hydroxymethylbilane synthase, More, PBG deaminase, PBG-D, PBG-deaminase, PBGD, PGB-D, porphobilinogen ammonia-lyase (polymerizing), porphobilinogen deaminase, pre-uroporphyrinogen synthase, preuroporphyrinogen synthase, preuroporphyrinogen synthetase, synthase, uroporphyrinogen I, uPBGD, UPGI-S, URO-S, urogenI synthase, uroporphyrinogen I synthase, uroporphyrinogen I synthetase, uroporphyrinogen synthase, uroporphyrinogen synthetase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.61 hydroxymethylbilane synthase

Cloned

Cloned on EC 2.5.1.61 - hydroxymethylbilane synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and sequencing of naturally occurring mutants G11R and R173Q
-
Escherichia coli BL21(DE3) pLysS with pUHD2 plasmid
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli strain BL21(DE3)
expression in Escherichia coli BL21(DE3) with pGEX-4T-1 expression vector
expression in Escherichia coli BL21+
expression of wild-type and mutant enzymes in SH-SY5Y neuroblastoma cells, expression analysis
-
expression of wild-type and mutants in Escherichia coli strain BL21
-
gene hemC, a single gene for PBGD in chromosome 5, recombinant expression of a codon-optimized PBGD gene in Escherichia coli
gene hemC, recombinant expression of His-tagged enzyme in Escherichia coli
gene hemC, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3)
Lambda ZAP Express system
-
localization of the porphobilinogen deaminase gene on chromosome 11q23.3
PBGD gene, DNA and amino acid sequence determination and analysis, expression of GST-tagged wild-type and mutant enzymes in Escherichia coli BL21 (DE3)
-
PCR-amplification, expression in Escherichia coli BL21Gold(DE3)(pLysS) with plasmid pET-14b(+)hemC
-
pTRE-EalAAT-hPBGD-WPRE plasmid from pTRE2 vector expressed in mice hepatocytes
recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) pLysS
SeMet-labelled enzyme
-
the enzyme is expressed into a housekeeping or an erythroid form as a result of differential promoter usage and splicing, three pairs of isoallelic forms, expression of two of the isoallelic forms, K210 and E210, with mutations involved in acute intermittent porphyria, AIP, in Escherichia coli as GST fusion proteins
-