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2.5.1.63: adenosyl-fluoride synthase

This is an abbreviated version!
For detailed information about adenosyl-fluoride synthase, go to the full flat file.

Word Map on EC 2.5.1.63

Reaction

S-adenosyl-L-methionine
+
fluoride
=
5'-deoxy-5'-fluoroadenosine
 +
L-methionine

Synonyms

5'-deoxy-5'-fluoroadenosine synthase, 5'-FDA synthase, 5'-FDAS, 5'-fluoro-5'-deoxy adenosine synthetase, 5'-fluoro-5'-deoxyadenosine synthase, 5’-fluorodeoxyadenosine synthase, adenosyl-fluoride synthase, FDAS, flA, flA protein, FlA1, FlA4, fluorinase, fluorinase enzyme, fluorinase, S-adenosyl-L-methionine, fluorination enzyme, NobA, SAM fluorinase, SAMN04487820_1174

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.63 adenosyl-fluoride synthase

Cloned

Cloned on EC 2.5.1.63 - adenosyl-fluoride synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli BL21
-
expression in Escherichia coli BL21(DE3)
-
expression in Escherichichia coli
expression of His-tagged enzyme in Escherichia coli BL21(DE3) pLysS with plasmid pET28b+
-
gene flA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type His6-tagged enzymes in Escherichia coli strain BL21(DE3)
gene flA, DNA sequence determination and analysis, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) and in strain B834(DE3), the latter results in the selenomethionine labeled enzyme
-
gene flA, genetic structure and organization
-
gene flA, genetic structure and organization, the flA gene is located in close proximity to the corresponding flB gene
gene flA, located at the center of the 10 kb gene Spencer cluster, genetic structure and organization, the f lA gene is located in close proximity to the corresponding f lB gene. Funtional recombinant expression in Salinispora tropica using gene replacement and replacing the gene salL that encodes chlorinase, EC 2.5.1.94, by gene flA from Streptomaces cattleya. The mutated Salinispora tropica strain produces fluorosalinosporamide
gene flA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-Gold (DE3) pLysS
gene nobA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant His6-tagged enzymes in Escherichia coli strain BL21(DE3)
mutant enzymes expressed in Escherichia coli C43 (DE3) cells
-