2.5.1.63: adenosyl-fluoride synthase

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For detailed information about adenosyl-fluoride synthase, go to the full flat file.

Reaction

Synonyms

5'-deoxy-5'-fluoroadenosine synthase, 5'-FDA synthase, 5'-FDAS, 5'-fluoro-5'-deoxy adenosine synthetase, 5'-fluoro-5'-deoxyadenosine synthase, 5�-fluorodeoxyadenosine synthase, adenosyl-fluoride synthase, FDAS, flA, flA protein, FlA1, FlA4, fluorinase, fluorinase enzyme, fluorinase, S-adenosyl-L-methionine, fluorination enzyme, NobA, SAM fluorinase, SAMN04487820_1174

ECTree

     2 Transferases

         2.5 Transferring alkyl or aryl groups, other than methyl groups

             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

                2.5.1.63 adenosyl-fluoride synthase

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Results	in table
3	Activating Compound
33	AA Sequence
18	Application
1	CAS Registry Number
16	Cloned(Commentary)
6	Crystallization (Commentary)
1	Expression
17	General Information
1	General Stability
18	Inhibitors
12	kcat/KM [mM/s]
1	Ki Value [mM]
31	KM Value [mM]
6	Metals/Ions
6	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
54	Organism
1	Pathway
51	PDB ID
10	pH Optimum
3	pH Range
27	Protein Variants
9	Purification (Commentary)
6	Reaction
2	Reaction Type
46	Reference
1	Source Tissue
2	Specific Activity [micromol/min/mg]
3	Storage Stability
70	Substrates and Products (Substrate)
8	Subunits
54	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
29	Turnover Number [1/s]

Purification

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Purification on EC 2.5.1.63 - adenosyl-fluoride synthase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Streptomyces cattleya

-

637250, 703173

nickel-affinity column

Streptomyces cattleya

-

709552

recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration

Streptomyces cattleya

-

660001

recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) pLysS by nickel affinity chromatography and gel filtration

Streptomyces cattleya

F8JPG4

738889

recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold(DE3)pLysS by nickel affinity chromatography and gel filtration

Streptomyces cattleya

F8JPG4

738890

recombinant wild-type and mutant His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Nocardia brasiliensis

W8JNL4

738197

recombinant wild-type His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Streptomyces cattleya

Q70GK9

738197

recovery from inclusion bodies after heterologous expression in Escherichia coli

Actinopolyspora mzabensis

A0A1G9FQX8

760084