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2.5.1.65: O-phosphoserine sulfhydrylase

This is an abbreviated version!
For detailed information about O-phosphoserine sulfhydrylase, go to the full flat file.

Word Map on EC 2.5.1.65

Reaction

O-phospho-L-serine
+
hydrogen sulfide
=
L-cysteine
+
phosphate

Synonyms

APE1586, ApOPSS, CysK2, CysM, O-acetyl-L-serine sulfhydrylase, O-acetylserine sulfhydrylase, O-phospho-L-serine sulfhydrylase, O-phospho-L-serine-dependent S-sulfocysteine synthase, O-phosphoserine S-sulfocysteine synthase, O-phosphoserine specific cysteine synthase, O-phosphoserine(thiol)-lyase, OASS, OPSS, Rv1336, S-sulfocysteine synthase

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.65 O-phosphoserine sulfhydrylase

Cloned

Cloned on EC 2.5.1.65 - O-phosphoserine sulfhydrylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli Rosetta (DE3)
-
expression in Escherichia coli Rosetta (DE3), sequencing
-
expression of wild-type, selenomethinonine-labeled, and mutant R297A enzymes in Escherichia coli
-
gene cysK2 or Rv0848, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
overexpression in Escherichia coli
-
overexpression in Escherichia coli Rosetta (DE3)
-
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS